Abstract:
Repeated treatments of Leishmania mexicana promastigote cultures with α-difluoromethylornithine could not block proliferation when the parasite was grown in a rich medium. Although the irreversible inhibitor of ornithine decarboxylase was able to abolish the enzymatic activity under these conditions, polyamine depletion was only partial probably due to the uptake of these substances from the external medium. Conversely, when Leishmania was cultivated in a defined medium essentially free of polyamines, α-difluoromethylornithine was able to decrease the growth rate and proliferation was arrested after several passages in the presence of the drug. Parasite multiplication could be resumed by addition of exogenous polyamines, and a strict correlation between Leishmania promastigote growth and intracellular levels of spermidine was observed. © 1991 Academic Press, Inc.
Registro:
Documento: |
Artículo
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Título: | Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels |
Autor: | Gonzalez, N.S.; Sanchez, C.P.; Sferco, L.; Algranati, I.D. |
Filiación: | Instituto de Investigaciones Bioquimicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, A. Machado 151, 1405 Buenos Aires, Argentina
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Palabras clave: | polyamine; article; cell level; growth regulation; leishmania mexicana; nonhuman; priority journal; Animal; Eflornithine; Kinetics; Leishmania mexicana; Ornithine Decarboxylase; Polyamines; Reproduction; Support, Non-U.S. Gov't; Leishmania mexicana |
Año: | 1991
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Volumen: | 180
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Número: | 2
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Página de inicio: | 797
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Página de fin: | 804
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DOI: |
http://dx.doi.org/10.1016/S0006-291X(05)81135-0 |
Título revista: | Biochemical and Biophysical Research Communications
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Título revista abreviado: | Biochem. Biophys. Res. Commun.
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ISSN: | 0006291X
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CODEN: | BBRCA
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CAS: | Eflornithine, 70052-12-9; Ornithine Decarboxylase, EC 4.1.1.17; Polyamines
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v180_n2_p797_Gonzalez |
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Citas:
---------- APA ----------
Gonzalez, N.S., Sanchez, C.P., Sferco, L. & Algranati, I.D.
(1991)
. Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels. Biochemical and Biophysical Research Communications, 180(2), 797-804.
http://dx.doi.org/10.1016/S0006-291X(05)81135-0---------- CHICAGO ----------
Gonzalez, N.S., Sanchez, C.P., Sferco, L., Algranati, I.D.
"Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels"
. Biochemical and Biophysical Research Communications 180, no. 2
(1991) : 797-804.
http://dx.doi.org/10.1016/S0006-291X(05)81135-0---------- MLA ----------
Gonzalez, N.S., Sanchez, C.P., Sferco, L., Algranati, I.D.
"Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels"
. Biochemical and Biophysical Research Communications, vol. 180, no. 2, 1991, pp. 797-804.
http://dx.doi.org/10.1016/S0006-291X(05)81135-0---------- VANCOUVER ----------
Gonzalez, N.S., Sanchez, C.P., Sferco, L., Algranati, I.D. Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels. Biochem. Biophys. Res. Commun. 1991;180(2):797-804.
http://dx.doi.org/10.1016/S0006-291X(05)81135-0