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Abstract:

Cuticle proteins of an insect pest, the Medfly Ceratitis capitata, were resolved in polyacrylamide gels and partially characterized. The pupal cuticle was found to be different from cuticles of other insects since more than 80% w w of the protein is a single mannose-containing polypeptide (PCG-100). The temporally-regulated in vivo biosynthesis and deposition of cuticle proteins was studied by microinjection of [35S]methionine followed by hand dissection of pupal cuticles. The major pupal glycoprotein, PCG-100, is cuticle- and stage-specific and was the earliest to be labeled and deposited. Its synthesis was maximal at around 46 hours after pupariation and then it decreased. The deposited PCG-100 and other minor pupal cuticle proteins become non-extractable at the end of the instar (7 days after pupariation) probably by sclerotization phenomena. These results provide insight into the temporal control of gene expression programs involved in cuticle deposition during medfly metamorphosis. © 1989.

Registro:

Documento: Artículo
Título:In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata
Autor:Boccaccio, G.L.; Quesada-Allué, L.A.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Campomar Universidad de Buenos Aires, Antonio Machado 151, 1405 Buenos Aires, Argentina
Facultad de Ciencias Exactas, Naturales-Universidad de Buenos Aires, Antonio Machado 151, 1405 Buenos Aires, Argentina
Palabras clave:animal cell; arthropod; ceratitis capitata; cuticle protein; glycoprotein synthesis; insect cuticle; metamorphosis; nonhuman; priority journal; Animal; Electrophoresis, Polyacrylamide Gel; Gene Expression Regulation; Glycoproteins; Insects; Metamorphosis, Biological; Proteins; Support, Non-U.S. Gov't
Año:1989
Volumen:164
Número:1
Página de inicio:251
Página de fin:258
DOI: http://dx.doi.org/10.1016/0006-291X(89)91710-5
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:cuticle proteins, insects; Glycoproteins; Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v164_n1_p251_Boccaccio

Referencias:

  • Quesada-Allué, (1987) Chagas' Disease Vectors, 2, pp. 77-94. , R.R. Brenner, A. Stoka, Second Edition, CRC Press, Boca Ratón
  • Andersen, Biochemistry of Insect Cuticle (1979) Annual Review of Entomology, 24, pp. 29-61
  • Doctor, Fristrom, Fristrom, (1985) J. Cell. Biol, 101, pp. 189-200
  • Yund, (1979) Mol. Cell. Endocrinol, 14, pp. 19-24
  • Fristrom, Doctor, Fristrom, Logan, Silvert, (1982) Devel. Biol, 91, pp. 337-350
  • Chihara, Silvert, Fristrom, (1982) Devel. Biol, 89, pp. 379-388
  • Kimbrell, Berger, King, Wolfgang, Fristrom, (1988) Insect. Biochem, 18, pp. 229-235
  • Souliotis, Patrinou-Georgoula, Zongza, Dimitriadis, (1988) Insect. Biochem, 18, pp. 485-492
  • Teran, (1977) Rev. Agronom. N.O. Argent, 14, pp. 1-4
  • Boccaccio, Quesada-Allué, (1989) Anales Asoc. Quím. Arg, 77, pp. 79-88
  • Ball, (1986) Anal. Biochem, 155, pp. 23-27
  • Laemmli, (1970) Nature, 227, pp. 680-683
  • Fairbanks, Steck, Wallach, (1971) Biochem, 10 (13), pp. 2606-2609
  • Faye, Chrispeels, (1985) Anal. Biochem, 149, pp. 218-224
  • Hackman, (1976) The Insect Integument, pp. 107-122. , H.R. Hepburn, Elsevier, Amsterdam
  • Silvert, Doctor, Quesada, Fristrom, (1984) Biochem, 23, pp. 5767-5774
  • Skelly, Howells, (1987) Insect Biochem, 17, pp. 625-633
  • Wright, (1987) Molecular Genetics of development, pp. 127-222. , J.R. Scandalios, Acad. Press, NY
  • Gnatzy, Romer, (1984) Biology of the Integument, 1, pp. 638-664. , Bereiter-Hahn et al., Second Edition, Springer-Verlag, Berlin, Invertebrates
  • Lifschitz, (1985) Annex, 2. , Second Edition, Report on Research Coordination Meeting, IAEA, Vienna
  • Roseland, Schneiderman, (1979) Wilhem Roux's Arch. Devel. Biol, 186, pp. 235-265
  • Neville, (1975) Biology of the Arthropod Cuticle, , Second Edition, Springer-Verlag. New York Inc
  • Roter, Spoffor, Swift, Synthesis of the major adult cuticle proteins of Drosophila melanogaster during hypoderm differentiation (1985) Developmental Biology, 107, pp. 615-619
  • Willis, Cuticular proteins: The neglected component (1987) Archives of Insect Biochemistry and Physiology, 6, pp. 203-215

Citas:

---------- APA ----------
Boccaccio, G.L. & Quesada-Allué, L.A. (1989) . In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata. Biochemical and Biophysical Research Communications, 164(1), 251-258.
http://dx.doi.org/10.1016/0006-291X(89)91710-5
---------- CHICAGO ----------
Boccaccio, G.L., Quesada-Allué, L.A. "In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata" . Biochemical and Biophysical Research Communications 164, no. 1 (1989) : 251-258.
http://dx.doi.org/10.1016/0006-291X(89)91710-5
---------- MLA ----------
Boccaccio, G.L., Quesada-Allué, L.A. "In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata" . Biochemical and Biophysical Research Communications, vol. 164, no. 1, 1989, pp. 251-258.
http://dx.doi.org/10.1016/0006-291X(89)91710-5
---------- VANCOUVER ----------
Boccaccio, G.L., Quesada-Allué, L.A. In vivo biosynthesis of a stage-specific cuticle glycoprotein during early metamorphosis of the medfly Ceratitis capitata. Biochem. Biophys. Res. Commun. 1989;164(1):251-258.
http://dx.doi.org/10.1016/0006-291X(89)91710-5