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Sanchez, C.P.; Gonzalez, N.S.; Algranati, I.D. "Stable ornithine decarboxylase in promastigotes of Leishmania mexicana" (1989) Biochemical and Biophysical Research Communications. 161(2):754-761
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Abstract:

Studies on the decarboxylation of ornithine in Leishmania mexicana have shown that this activity corresponds to a true ornithine decarboxylase rather than to an oxidative decarboxylation or aminotransferase reaction, both of which also give rise to the release of CO2. The stoichiometric relationship between substrate and products has indicated that extracts of L. mexicana were able to catalyse the formation of an unknown compound besides putrescine and CO2. The addition of cycloheximide to cultures of L. mexicana allowed us to demonstrate that ornithine decarboxylase degradation in vivo was extremely slow in this parasite. This remarkable stability of the enzyme is only comparable to that found in Trypanosoma brucei and contrasts with the high turnover rate of ornithine decarboxylases of different mammalian cells. © 1989.

Registro:

Documento: Artículo
Título:Stable ornithine decarboxylase in promastigotes of Leishmania mexicana
Autor:Sanchez, C.P.; Gonzalez, N.S.; Algranati, I.D.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires A. Machado 151, 1405 Buenos Aires, Argentina
CONICET, A. Machado 151, 1405 Buenos Aires, Argentina
Palabras clave:ornithine decarboxylase; leishmania mexicana; nonhuman; priority journal; protein stability; protozoon; Animal; Carboxy-Lyases; Cell-Free System; Kinetics; Leishmania mexicana; Ornithine Decarboxylase; Protein Denaturation; Pyridoxal Phosphate; Substrate Specificity; Support, Non-U.S. Gov't
Año:1989
Volumen:161
Número:2
Página de inicio:754
Página de fin:761
DOI: http://dx.doi.org/10.1016/0006-291X(89)92664-8
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:ornithine decarboxylase, 9024-60-6; arginine decarboxylase, EC 4.1.1.19; Carboxy-Lyases, EC 4.1.1.; Ornithine Decarboxylase, EC 4.1.1.17; Pyridoxal Phosphate, 54-47-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v161_n2_p754_Sanchez

Referencias:

  • Jänne, Pössö, Raina, (1978) Biochim. Biophys. Acta, 473, pp. 241-293
  • Pegg, (1988) Cancer Res, 48, pp. 759-774
  • Heby, (1981) Differentiation, 19, pp. 1-20
  • Sunkara, Baylin, Luck, (1987) Inhibition of Polyamine Metabolism. Biological Significance and Basis for New Therapies, pp. 121-140. , P.P. McCann, A.E. Pegg, A. Sjoerdsma, Academic Press, Orlando, FL
  • Talpaz, Plager, Quesda, Benjamin, Kantarjian, Gutterman, (1986) Eur. J. Cancer Clin. Oncol, 22, pp. 685-689
  • Bacchi, Nathan, Hutner, McCann, (1980) Science, 210, pp. 332-334
  • Hanson, Bradford, Chapman, Waits, McCann, Sjoerdsma, (1982) Am. J. Vet. Res, 43, pp. 1651-1653
  • Assaraf, Golenser, Spira, Bachrach, (1984) Biochem. J, 222, pp. 815-819
  • Bacchi, McCann, (1987) Inhibition of Polyamine Metabolism. Biological Significance and Basis for New Therapies, pp. 317-344. , P.P. McCann, A.E. Pegg, A. Sjoerdsma, Academic Press, Orlando, FL
  • Phillips, Coffino, Wang, (1987) J. Biol. Chem, 262, pp. 8721-8727
  • Warren, (1960) J. Parasitol, 46, pp. 529-538
  • Cataldi, Algranati, Polyamines and regulation of ornithine biosynthesis in Escherichia coli. (1989) J Bacteriol, , in press
  • Camargo, Coelho, Moraes, Figuereido, Trypanosoma spp., Leishmania spp. and Leptomonas spp.: Enzymes of ornithine-arginine metabolism (1978) Experimental Parasitology, 46, pp. 141-144
  • Smith, Marshall, (1988) Phytochemistry, 27, pp. 703-710
  • Murphy, Brosnan, (1976) Biochem. J, 157, pp. 33-39
  • Kim, Sobota, Bitonti, McCann, Byers, (1987) J. Protozool, 34, pp. 278-284
  • Metcalf, Bey, Danzing, Jung, Casara, Vevert, (1978) J. Am. Chem. Soc, 100, pp. 2551-2553
  • Kitz, Wilson, (1962) J. Biol. Chem, 237, pp. 3245-3249
  • Kanamoto, Utsonomiya, Kameji, Hayashi, (1986) Eur. J. Biochem, 154, pp. 539-544
  • Pegg, (1986) Biochem. J, 234, pp. 249-262
  • Rogers, Wells, Rechsteiner, (1986) Science, 234, pp. 364-368

Citas:

---------- APA ----------
Sanchez, C.P., Gonzalez, N.S. & Algranati, I.D. (1989) . Stable ornithine decarboxylase in promastigotes of Leishmania mexicana. Biochemical and Biophysical Research Communications, 161(2), 754-761.
http://dx.doi.org/10.1016/0006-291X(89)92664-8
---------- CHICAGO ----------
Sanchez, C.P., Gonzalez, N.S., Algranati, I.D. "Stable ornithine decarboxylase in promastigotes of Leishmania mexicana" . Biochemical and Biophysical Research Communications 161, no. 2 (1989) : 754-761.
http://dx.doi.org/10.1016/0006-291X(89)92664-8
---------- MLA ----------
Sanchez, C.P., Gonzalez, N.S., Algranati, I.D. "Stable ornithine decarboxylase in promastigotes of Leishmania mexicana" . Biochemical and Biophysical Research Communications, vol. 161, no. 2, 1989, pp. 754-761.
http://dx.doi.org/10.1016/0006-291X(89)92664-8
---------- VANCOUVER ----------
Sanchez, C.P., Gonzalez, N.S., Algranati, I.D. Stable ornithine decarboxylase in promastigotes of Leishmania mexicana. Biochem. Biophys. Res. Commun. 1989;161(2):754-761.
http://dx.doi.org/10.1016/0006-291X(89)92664-8