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Fernandez de Recondo, M.E.; Legorburu, C.; Monge, J.C.; Recondo, E.F. "Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity" (1988) Biochemical and Biophysical Research Communications. 155(1):216-223
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Abstract:

Heparin and Concanavalin A complexes were studied under different conditions. Interaction was measured by reading the turbidity at 420 nm. The influence of the pH, heparin, and salt concentration was measured. In the presence of salts pH was very critical, and above pH 5.4 the interaction was practically negligible. At pH 4.6 or 5.2 and the lowest salt concentration compatible with buffering, heparin fractions with different anticoagulant specific activities were detected in the precipitate and in the supernatant, after the interaction. In all cases a significative difference was observed in favor of the heparin isolated from the precipitate. Possibility of an artifact was eliminated by using adequates blanks and running the coagulation tests in the presence of an excess of Concanavalin A. © 1988 Academic Press, Inc.

Registro:

Documento: Artículo
Título:Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity
Autor:Fernandez de Recondo, M.E.; Legorburu, C.; Monge, J.C.; Recondo, E.F.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Instituto de Química Biológica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Argentina
Palabras clave:concanavalin a; heparin; anticoagulation; chemical interaction; priority journal; Blood Coagulation Tests; Calibration; Concanavalin A; Drug Interactions; Fractional Precipitation; Heparin; Hydrogen-Ion Concentration; Salts; Support, Non-U.S. Gov't
Año:1988
Volumen:155
Número:1
Página de inicio:216
Página de fin:223
DOI: http://dx.doi.org/10.1016/S0006-291X(88)81071-4
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:concanavalin A, 11028-71-0; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; Concanavalin A, 11028-71-0; Heparin, 9005-49-6; Salts
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v155_n1_p216_FernandezdeRecondo

Referencias:

  • Summer, Howell, (1936) J. Bacteriol., 32, p. 227237
  • Summer, Howell, (1936) J. Biol. Chem., 115, pp. 583-588
  • Cifonelli, Montgomery, Smith, (1956) J. Amer. Chem. Soc., 78, pp. 2485-2488
  • Cifonelli, Montgomery, Smith, (1956) J. Amer. Chem. Soc., 78, pp. 2488-2489
  • Goldstein, Hollerman, Merrick, (1965) Biochim. Biophys. Acta., 97, pp. 68-76
  • Goldstein, Hollerman, Smith, (1965) Biochemistry, 4, pp. 876-883. , Nro 5
  • Smith, Goldstein, (1967) Arch. Biochem. Biophys., 121, pp. 88-95
  • So, Goldstein, (1968) Biochim. Biophys. Acta, 165, pp. 398-404
  • So, Goldstein, (1967) J. Biol. Chem., 242, pp. 1617-1622
  • Doyle, Kan, (1972) FEBS LETT., 20, pp. 22-24. , Nro 1
  • Rodriguez Montelongo, Fernández de Recondo, Recondo, (1986) Acta Bioquim. Clin. Lat. Amer., 20, pp. 433-447. , Nro 3
  • Freeman, Engelberg, Dudley, (1954) Proc. Natl. Acad. Sci., 24, pp. 79-86
  • Yin, Wessler, Butler, (1973) J. Lab. Clin. Med., 81, pp. 298-300
  • Lam, Silbert, Rosenberg, (1976) Biochem. Biophys. Res. Commun., 69, pp. 570-577
  • Thunberg, Bäckström, Grundberg, Riesenfeld, Lindahl, (1980) FEBS Lett., 117, pp. 203-206
  • Riesenfeld, Thunberg, Hook, Lindahl, (1981) J. Biol. Chem., 256, pp. 2389-2394
  • Radoff, Danishefsky, (1984) J. Biol. Chem., 259 (1), pp. 166-172

Citas:

---------- APA ----------
Fernandez de Recondo, M.E., Legorburu, C., Monge, J.C. & Recondo, E.F. (1988) . Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity. Biochemical and Biophysical Research Communications, 155(1), 216-223.
http://dx.doi.org/10.1016/S0006-291X(88)81071-4
---------- CHICAGO ----------
Fernandez de Recondo, M.E., Legorburu, C., Monge, J.C., Recondo, E.F. "Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity" . Biochemical and Biophysical Research Communications 155, no. 1 (1988) : 216-223.
http://dx.doi.org/10.1016/S0006-291X(88)81071-4
---------- MLA ----------
Fernandez de Recondo, M.E., Legorburu, C., Monge, J.C., Recondo, E.F. "Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity" . Biochemical and Biophysical Research Communications, vol. 155, no. 1, 1988, pp. 216-223.
http://dx.doi.org/10.1016/S0006-291X(88)81071-4
---------- VANCOUVER ----------
Fernandez de Recondo, M.E., Legorburu, C., Monge, J.C., Recondo, E.F. Heparin and concanavalin A interaction: Isolation of fraction with higher anticoagulant activity. Biochem. Biophys. Res. Commun. 1988;155(1):216-223.
http://dx.doi.org/10.1016/S0006-291X(88)81071-4