The effect of streptomycin and other aminoglycosides on protein synthesis has been studied using various streptomycin-sensitive strains unable to synthesize polyamines. We have confirmed and extended our previous results showing that the strong inhibition of translation caused by the antibiotic in polyamine-supplemented bacteria was markedly reduced in polyamine-starved cells. The analysis of polypeptides synthesized in the absence and presence of streptomycin in bacteria grown with and without putrescine has shown that the antibiotic provoked the accumulation of low molecular weight peptides partially bound to ribosomes in polyamine-unstarved cells. On the contrary, the drug did not induce major alterations in the patterns of proteins obtained from polyamine-depleted bacteria. The addition of the antibiotic did not evoke any change of proteolytic activity. © 1988.
Documento: | Artículo |
Título: | Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria |
Autor: | Nastri, H.G.; Algranati, I.D. |
Filiación: | Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, A. Machado 151, 1405 Buenos Aires, Argentina CONICET, A. Machado 151, 1405 Buenos Aires, Argentina |
Palabras clave: | kanamycin; neomycin; putrescine; streptomycin; escherichia coli; protein synthesis; Antibiotics, Aminoglycoside; Bacterial Proteins; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Kinetics; Molecular Weight; Polyamines; Putrescine; Streptomycin; Succinates; Succinic Acid; Support, Non-U.S. Gov't; Translation, Genetic |
Año: | 1988 |
Volumen: | 150 |
Número: | 3 |
Página de inicio: | 947 |
Página de fin: | 954 |
DOI: | http://dx.doi.org/10.1016/0006-291X(88)90721-8 |
Título revista: | Biochemical and Biophysical Research Communications |
Título revista abreviado: | Biochem. Biophys. Res. Commun. |
ISSN: | 0006291X |
CODEN: | BBRCA |
CAS: | kanamycin, 11025-66-4, 61230-38-4, 8063-07-8; neomycin, 11004-65-2, 1404-04-2, 1405-10-3, 8026-22-0; putrescine, 110-60-1, 333-93-7; streptomycin, 57-92-1; Antibiotics, Aminoglycoside; Bacterial Proteins; Polyamines; Putrescine, 110-60-1; Streptomycin, 57-92-1; Succinates; Succinic Acid, 110-15-6 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v150_n3_p947_Nastri |