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Abstract:

The effect of streptomycin and other aminoglycosides on protein synthesis has been studied using various streptomycin-sensitive strains unable to synthesize polyamines. We have confirmed and extended our previous results showing that the strong inhibition of translation caused by the antibiotic in polyamine-supplemented bacteria was markedly reduced in polyamine-starved cells. The analysis of polypeptides synthesized in the absence and presence of streptomycin in bacteria grown with and without putrescine has shown that the antibiotic provoked the accumulation of low molecular weight peptides partially bound to ribosomes in polyamine-unstarved cells. On the contrary, the drug did not induce major alterations in the patterns of proteins obtained from polyamine-depleted bacteria. The addition of the antibiotic did not evoke any change of proteolytic activity. © 1988.

Registro:

Documento: Artículo
Título:Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria
Autor:Nastri, H.G.; Algranati, I.D.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, A. Machado 151, 1405 Buenos Aires, Argentina
CONICET, A. Machado 151, 1405 Buenos Aires, Argentina
Palabras clave:kanamycin; neomycin; putrescine; streptomycin; escherichia coli; protein synthesis; Antibiotics, Aminoglycoside; Bacterial Proteins; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Kinetics; Molecular Weight; Polyamines; Putrescine; Streptomycin; Succinates; Succinic Acid; Support, Non-U.S. Gov't; Translation, Genetic
Año:1988
Volumen:150
Número:3
Página de inicio:947
Página de fin:954
DOI: http://dx.doi.org/10.1016/0006-291X(88)90721-8
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:kanamycin, 11025-66-4, 61230-38-4, 8063-07-8; neomycin, 11004-65-2, 1404-04-2, 1405-10-3, 8026-22-0; putrescine, 110-60-1, 333-93-7; streptomycin, 57-92-1; Antibiotics, Aminoglycoside; Bacterial Proteins; Polyamines; Putrescine, 110-60-1; Streptomycin, 57-92-1; Succinates; Succinic Acid, 110-15-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v150_n3_p947_Nastri

Referencias:

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Citas:

---------- APA ----------
Nastri, H.G. & Algranati, I.D. (1988) . Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria. Biochemical and Biophysical Research Communications, 150(3), 947-954.
http://dx.doi.org/10.1016/0006-291X(88)90721-8
---------- CHICAGO ----------
Nastri, H.G., Algranati, I.D. "Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria" . Biochemical and Biophysical Research Communications 150, no. 3 (1988) : 947-954.
http://dx.doi.org/10.1016/0006-291X(88)90721-8
---------- MLA ----------
Nastri, H.G., Algranati, I.D. "Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria" . Biochemical and Biophysical Research Communications, vol. 150, no. 3, 1988, pp. 947-954.
http://dx.doi.org/10.1016/0006-291X(88)90721-8
---------- VANCOUVER ----------
Nastri, H.G., Algranati, I.D. Inhibition of protein synthesis by aminoglycoside antibiotics in polyamine-requiring bacteria. Biochem. Biophys. Res. Commun. 1988;150(3):947-954.
http://dx.doi.org/10.1016/0006-291X(88)90721-8