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Abstract:

EDTA-treated Rhizobium trifolii cells (strain NA30) incorporate radioactivity from (14C) labeled UDP-Clc, UDP-ClcA, Acetyl-Coa and/or phosphoenol pyruvate into chloroform:methanol:water (1:2:0.3) extracts. The incorporation products have properties of prenyl-phospho-sugars; mild alkaline hydrolysis of these extracts produce cyclic phosphate esters suggesting the presence of a diphosphate bridge, and mild acid or catalytic reduction-alkaline phosphatase treatments release four main componenets a, b, c and d, as judged by paper electrophoresis and chromatography and gel filtration studies. The four components can be obtained (14C)acetyl-labeled, but only compound c and to a lesser degree compound b can be (14C)pyruvate-labeled. For the exopolysaccharide produced by this strain the following repeating unit has been proposed (Robertsen et al. (1981), Plant Physiol. 67, 389-400): {A figure is presented}. The results obtained suggest that the octasaccharide repeating unit (compound a) with one (compound b) or two (compound c) ketal pyruvate residues are assembled on a lipid acceptor. All these compounds are assumed to be intermediates in the biosynthesis of R. trifolii exopolysaccharide. © 1986.

Registro:

Documento: Artículo
Título:In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
Autor:Bossio, J.C.; Inon de Iannino, N.; Dankert, M.A.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales (UBA), 1405 Buenos Aires, Argentina
Consejo Nacional de Investigaciones Cientificas y Técnicas Patricias Argentinas 435, 1405 Buenos Aires, Argentina
Palabras clave:acetic acid; lipid; oligosaccharide; pyruvic acid; nonhuman; priority journal; rhizobium trifolii; Acetylation; Carbohydrate Sequence; Chromatography, Gel; Electrophoresis, Paper; Lipids; Oligosaccharides; Pyruvates; Pyruvic Acid; Rhizobium; Support, Non-U.S. Gov't
Año:1986
Volumen:134
Número:1
Página de inicio:205
Página de fin:211
DOI: http://dx.doi.org/10.1016/0006-291X(86)90548-6
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; lipid, 66455-18-3; pyruvic acid, 127-17-3, 19071-34-2, 57-60-3; Lipids; Oligosaccharides; Pyruvates; Pyruvic Acid, 127-17-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v134_n1_p205_Bossio

Referencias:

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  • Albersheim, Darvill, Davis, Lau, McNeil, Sharp, York, (1984) Structure, Function and Biosynthesis of Plant Cell Walls, pp. 19-51. , W.M. Dugger, S. Bartnicki-García
  • Tolmasky, Staneloni, Ugalde, Leloir, (1980) Arch. Biochem. Biophys, 203, pp. 358-364
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  • Staneloni, Tolmasky, Leloir, (1984) J. Gen. Microbiol, 130, pp. 869-879
  • Robertsen, Aman, Darvill, McNeil, Albersheim, Host-Symbiont Interactions : V. THE STRUCTURE OF ACIDIC EXTRACELLULAR POLYSACCHARIDES SECRETED BY RHIZOBIUM LEGUMINOSARUM AND RHIZOBIUM TRIFOLII (1981) PLANT PHYSIOLOGY, 67, pp. 389-400
  • Couso, Ielpi, Dankert, (1980) Arch. Biochem. Biophys, 204, pp. 434-443
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  • Pont Lezica, R., Brett, C.T., Romero, P. and Dankert, M.A. Biochem. Biophys. Res. Commun. 66, 980-987; Trevelyan, Procter, Harrison, (1950) Nature (London), 166, pp. 444-445
  • Amemura, Harada, (1983) Carbohydr. Res, 112, pp. 85-93
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Citas:

---------- APA ----------
Bossio, J.C., Inon de Iannino, N. & Dankert, M.A. (1986) . In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii. Biochemical and Biophysical Research Communications, 134(1), 205-211.
http://dx.doi.org/10.1016/0006-291X(86)90548-6
---------- CHICAGO ----------
Bossio, J.C., Inon de Iannino, N., Dankert, M.A. "In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii" . Biochemical and Biophysical Research Communications 134, no. 1 (1986) : 205-211.
http://dx.doi.org/10.1016/0006-291X(86)90548-6
---------- MLA ----------
Bossio, J.C., Inon de Iannino, N., Dankert, M.A. "In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii" . Biochemical and Biophysical Research Communications, vol. 134, no. 1, 1986, pp. 205-211.
http://dx.doi.org/10.1016/0006-291X(86)90548-6
---------- VANCOUVER ----------
Bossio, J.C., Inon de Iannino, N., Dankert, M.A. In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii. Biochem. Biophys. Res. Commun. 1986;134(1):205-211.
http://dx.doi.org/10.1016/0006-291X(86)90548-6