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Abstract:

Ribosomes from stationary phase Bacillus stearothermophilus have a diminished activity in protein synthesis due to the presence of a bound protein (association factor I, AFI). This factor inhibits the binding of fmet-tRNA to ribosomes. The inhibition of protein synthesis can be reversed in systems directed by viral mRNA by the addition of an excess of high salt ribosomal wash obtained from mid-log cells. Under this condition AFI is released from ribosomes. © 1982.

Registro:

Documento: Artículo
Título:The reactivation of stationary phase ribosomes from Bacillus stearothermophilus
Autor:García-Patrone, M.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, Obligado 2490, 1428 Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:animal experiment; bacillus stearothermophilus; in vitro study; ribosome; Bacillus stearothermophilus; Bacteriophages; Cell Cycle; Hypertonic Solutions; Peptide Initiation Factors; Poly U; Proteins; Ribosomal Proteins; Ribosomes; RNA, Messenger; RNA, Viral; Support, Non-U.S. Gov't
Año:1982
Volumen:104
Número:4
Página de inicio:1413
Página de fin:1420
DOI: http://dx.doi.org/10.1016/0006-291X(82)91407-3
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:Hypertonic Solutions; Peptide Initiation Factors; Poly U, 27416-86-0; Proteins; Ribosomal Proteins; RNA, Messenger; RNA, Viral
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v104_n4_p1413_GarciaPatrone

Referencias:

  • Li, Umbreit, (1966) Biochim. Biophys. Acta, 119, pp. 392-399
  • Scheps, Wax, Revel, (1971) Biochim. Biophys. Acta, 232, pp. 140-150
  • González, Goldemberg, Algranati, (1968) Biochim. Biophys. Acta, 166, pp. 760-762
  • García-Patrone, Polypeptide synthesis inhibition by a factor inducing stabilization of 30 S-50 S ribosomal couples (1978) FEBS Letters, 92, pp. 263-268
  • García-Patrone, Mechanism of protein synthesis inhibition during stationary phase in Bacillus stearothermophil US (1980) Molecular and Cellular Biochemistry, 31, pp. 97-103
  • Kay, Graffe, Grunberg-Manago, (1976) Biochimie, 58, pp. 183-199
  • Minks, Suryanarayana, Subramanian, (1978) Eur. J. Biochem, 82, pp. 271-277
  • Studier, (1973) J. Mol. Biol, 79, pp. 237-248

Citas:

---------- APA ----------
(1982) . The reactivation of stationary phase ribosomes from Bacillus stearothermophilus. Biochemical and Biophysical Research Communications, 104(4), 1413-1420.
http://dx.doi.org/10.1016/0006-291X(82)91407-3
---------- CHICAGO ----------
García-Patrone, M. "The reactivation of stationary phase ribosomes from Bacillus stearothermophilus" . Biochemical and Biophysical Research Communications 104, no. 4 (1982) : 1413-1420.
http://dx.doi.org/10.1016/0006-291X(82)91407-3
---------- MLA ----------
García-Patrone, M. "The reactivation of stationary phase ribosomes from Bacillus stearothermophilus" . Biochemical and Biophysical Research Communications, vol. 104, no. 4, 1982, pp. 1413-1420.
http://dx.doi.org/10.1016/0006-291X(82)91407-3
---------- VANCOUVER ----------
García-Patrone, M. The reactivation of stationary phase ribosomes from Bacillus stearothermophilus. Biochem. Biophys. Res. Commun. 1982;104(4):1413-1420.
http://dx.doi.org/10.1016/0006-291X(82)91407-3