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Abstract:

Protein synthesis in a polyamine-auxotrophic mutant of E. coli cultivated in the absence and presence of putrescine has been studied. The ratio of in vivo polypeptide synthesis under both conditions was the same as that obtained with cell-free extracts derived from polyamine-depleted and supplemented bacteria. The markedly reduced activity in systems from polyamine starved cells is most probably due to a poorly efficient initiation step. The estimation of nascent peptide chains and their release from tRNA support the idea that polypeptide elongation and termination processes do not have decreased rates in systems from polyamine-depleted bacteria. © 1981.

Registro:

Documento: Artículo
Título:Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria
Autor:Algranati, I.D.; Goldemberg, S.H.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, Obligado 2490, 1428 Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:putrescine; spermidine; spermine; animal experiment; auxotrophic mutant; escherichia coli; heredity; in vitro study; protein synthesis; Cell-Free System; Escherichia coli; Kinetics; Mutation; Peptide Chain Elongation; Peptide Chain Initiation; Peptide Chain Termination; Putrescine; Ribosomes; RNA, Transfer, Amino Acyl; Support, Non-U.S. Gov't
Año:1981
Volumen:103
Número:1
Página de inicio:8
Página de fin:15
DOI: http://dx.doi.org/10.1016/0006-291X(81)91653-3
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9; spermine, 306-67-2, 71-44-3; Putrescine, 110-60-1; RNA, Transfer, Amino Acyl; tRNA, formylmethionine-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v103_n1_p8_Algranati

Referencias:

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Citas:

---------- APA ----------
Algranati, I.D. & Goldemberg, S.H. (1981) . Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria. Biochemical and Biophysical Research Communications, 103(1), 8-15.
http://dx.doi.org/10.1016/0006-291X(81)91653-3
---------- CHICAGO ----------
Algranati, I.D., Goldemberg, S.H. "Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria" . Biochemical and Biophysical Research Communications 103, no. 1 (1981) : 8-15.
http://dx.doi.org/10.1016/0006-291X(81)91653-3
---------- MLA ----------
Algranati, I.D., Goldemberg, S.H. "Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria" . Biochemical and Biophysical Research Communications, vol. 103, no. 1, 1981, pp. 8-15.
http://dx.doi.org/10.1016/0006-291X(81)91653-3
---------- VANCOUVER ----------
Algranati, I.D., Goldemberg, S.H. Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria. Biochem. Biophys. Res. Commun. 1981;103(1):8-15.
http://dx.doi.org/10.1016/0006-291X(81)91653-3