Artículo
Abstract:
Xanthan gum is a complex polysaccharide produced by Xanthomonas campestris, composed of pentasaccharide repeating units containing mannose, glucose and glucuronic acid (in the ratio 2:2:1) and pyruvic acid acetal and 0-acetyl residues in differing proportions Jansson et al. (1975) Carbohyd.Res. 45, 275-282). Previous work (Ielpi et al. FEBS Lett., in press) has shown that the pentasaccharide is first assembled on a pyrophosphate lipid and then polymerized to xanthan gum. It is now shown, using EDTA-treated cells and different combinations of the adequate [14C]labeled donors, that phosphoenol pyruvate provides the acetal residues and that the transfer occurs on the terminal mannose of the pentasaccharide-P-P-lipid, as judged by the solubility properties and DEAE-cellulose column chromatography of the compounds formed as well as by the behaviour of the substances liberated by mild acid and alkaline treatments and Smith degradation. The subsequent polymerization process leads to pyruvylated xanthan gum. © 1981 Academic Press, Inc.
Registro:
| Documento: | Artículo |
| Título: | Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid |
| Autor: | Ielpi, L.; Couso, R.O.; Dankert, M.A. |
| Filiación: | Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | bacterial polysaccharide; glycolipid; phosphoenolpyruvate; pyruvic acid; pyruvic acid derivative; xanthan; article; biosynthesis; ion exchange chromatography; metabolism; paper chromatography; Xanthomonas; Chromatography, DEAE-Cellulose; Chromatography, Paper; Glycolipids; Phosphoenolpyruvate; Polysaccharides, Bacterial; Pyruvates; Pyruvic Acid; Xanthomonas |
| Año: | 1981 |
| Volumen: | 102 |
| Número: | 4 |
| Página de inicio: | 1400 |
| Página de fin: | 1408 |
| DOI: | http://dx.doi.org/10.1016/S0006-291X(81)80167-2 |
| Título revista: | Biochemical and Biophysical Research Communications |
| Título revista abreviado: | Biochem. Biophys. Res. Commun. |
| ISSN: | 0006291X |
| CODEN: | BBRCA |
| CAS: | phosphoenolpyruvate, 33016-40-9, 5541-93-5, 73-89-2; pyruvic acid, 127-17-3, 19071-34-2, 57-60-3; xanthan, 11138-66-2; Glycolipids; Phosphoenolpyruvate, 73-89-2; Polysaccharides, Bacterial; Pyruvates; Pyruvic Acid, 127-17-3; xanthan gum, 11138-66-2 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v102_n4_p1400_Ielpi |
Referencias:
- Jansson, Kenne, Lindberg, (1975) Carbohyd. Res., 45, pp. 275-282
- Melton, Mindt, Rees, Sanderson, (1976) Carbohyd. Res., 46, pp. 245-257
- Ielpi, Couso, Dankert, Lipid-linked intermediates in the biosynthesis of xanthan gum. (1981) FEBS Lett, , (in press)
- Sutherland, (1979) Microbial Polysaccharides and Polysaccharides, pp. 1-34. , Academic Press, Heidelberg
- Garcia, Recondo, Dankert, (1974) Eur. J. Biochem., 43, pp. 93-105
- Couso, Ielpi, Garcia, Dankert, (1980) Arch. Biochem. Biophys., 204, pp. 434-443
- Cadmus, Rogovin, Burton, Pittsley, Knutson, Jeanes, (1976) Can. J. Microbiol., 22, pp. 942-948
- Lowry, Rosebrough, Farr, Randall, (1951) J. Biol. Chem., 193, pp. 265-275
- Goldstein, Hay, Lewis, Smith, Methods Carbohydr. Chem. (1975) Methods Carbohydr. Chem., 5, pp. 361-370
- Trevelyan, Procter, Harrison, (1950) Nature (London), 166, pp. 444-445
- Dankert, Wright, Kelley, Robbins, (1966) Arch. Biochem. Biophys., 116, pp. 425-435
Citas:
---------- APA ----------
Ielpi, L., Couso, R.O. & Dankert, M.A. (1981) . Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid. Biochemical and Biophysical Research Communications, 102(4), 1400-1408.http://dx.doi.org/10.1016/S0006-291X(81)80167-2
---------- CHICAGO ----------
Ielpi, L., Couso, R.O., Dankert, M.A. "Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid" . Biochemical and Biophysical Research Communications 102, no. 4 (1981) : 1400-1408.http://dx.doi.org/10.1016/S0006-291X(81)80167-2
---------- MLA ----------
Ielpi, L., Couso, R.O., Dankert, M.A. "Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid" . Biochemical and Biophysical Research Communications, vol. 102, no. 4, 1981, pp. 1400-1408.http://dx.doi.org/10.1016/S0006-291X(81)80167-2
---------- VANCOUVER ----------
Ielpi, L., Couso, R.O., Dankert, M.A. Xanthan cum biosynthesis pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid. Biochem. Biophys. Res. Commun. 1981;102(4):1400-1408.http://dx.doi.org/10.1016/S0006-291X(81)80167-2
