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Abstract:

Gel electrophoresis and sucrose density gradient centrifugation techniques permitted the visualization for the first time of the ternary complex formed by the binding of cAMP to Mucor rouxii cAMP-dependent protein kinase holoenzyme. The addition of 0.5 M NaCl or histone plus ATP-Mg++, together with cAMP, dissociates the holoenzyme into free regulatory (R) and catalytic (C) subunits. At 4°C, cAMP bound to the holoenzyme is readily exchangeable with unlabeled cAMP (half life 2.5 min), while the nucleotide bound to the R subunit has a very slow exchange rate (half life 210 min). The amount of cAMP bound to R subunit is approximately twice the amount bound to holoenzyme at saturation. © 1981.

Registro:

Documento: Artículo
Título:cAMP-dependent protein kinase from Mucor rouxii: Physical evidence of a ternary complex holoenzyme-cAMP
Autor:Pastori, R.L.; Kerner, N.; Moreno, S.; Passeron, S.
Filiación:Programa de Regulación Hormonal y Metabólica, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:cyclic AMP; protein kinase; article; density gradient centrifugation; enzymology; isolation and purification; macromolecule; metabolism; Mucor; protein binding; Centrifugation, Density Gradient; Cyclic AMP; Macromolecular Systems; Mucor; Protein Binding; Protein Kinases; Support, Non-U.S. Gov't
Año:1981
Volumen:101
Número:2
Página de inicio:663
Página de fin:671
DOI: http://dx.doi.org/10.1016/0006-291X(81)91310-3
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:cyclic AMP, 60-92-4; protein kinase, 9026-43-1; Cyclic AMP, 60-92-4; Macromolecular Systems; Protein Kinases, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v101_n2_p663_Pastori

Referencias:

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Citas:

---------- APA ----------
Pastori, R.L., Kerner, N., Moreno, S. & Passeron, S. (1981) . cAMP-dependent protein kinase from Mucor rouxii: Physical evidence of a ternary complex holoenzyme-cAMP. Biochemical and Biophysical Research Communications, 101(2), 663-671.
http://dx.doi.org/10.1016/0006-291X(81)91310-3
---------- CHICAGO ----------
Pastori, R.L., Kerner, N., Moreno, S., Passeron, S. "cAMP-dependent protein kinase from Mucor rouxii: Physical evidence of a ternary complex holoenzyme-cAMP" . Biochemical and Biophysical Research Communications 101, no. 2 (1981) : 663-671.
http://dx.doi.org/10.1016/0006-291X(81)91310-3
---------- MLA ----------
Pastori, R.L., Kerner, N., Moreno, S., Passeron, S. "cAMP-dependent protein kinase from Mucor rouxii: Physical evidence of a ternary complex holoenzyme-cAMP" . Biochemical and Biophysical Research Communications, vol. 101, no. 2, 1981, pp. 663-671.
http://dx.doi.org/10.1016/0006-291X(81)91310-3
---------- VANCOUVER ----------
Pastori, R.L., Kerner, N., Moreno, S., Passeron, S. cAMP-dependent protein kinase from Mucor rouxii: Physical evidence of a ternary complex holoenzyme-cAMP. Biochem. Biophys. Res. Commun. 1981;101(2):663-671.
http://dx.doi.org/10.1016/0006-291X(81)91310-3