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Speziale, N.; Speziale, E.H.; Pasquini, J.M. "Bradykinin stimulates phospholipase C in rat renal medullary slices" (1985) Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 836(1):14-18
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Abstract:

Rat renal medullary slices prelabeled with [14C]arachidonic acid generate [14C]diacylglycerol within 1 min of exposure to bradykinin action. Production of [14C]diacylglycerol is transient. 2 min after the addition of bradykinin, the levels of metabolite reach the maximun, but decrease thereafter. Simultaneously, bradykinin induces a parallel decrease of the radioactivity in phosphatidylinositol. No degradation of other phospholipids is observed, and triacylglycerol is not affected. The degradation of [14C]phosphatidylinositol to (14C]diacylglycerol indicated the presence of phospholipase C acivity. Preincubation of prelabeled slices with 2 mM dibutyryl cyclic AMP prevents both the generation of diacylglycerol and the degradation of phosphatidylinositol. Neither mepacrine nor indomethacin block diacylglycerol production and phosphatidylinositol breakdown. We conclude that, when rat renal medullary slices are stimulated with bradykinin, phosphatidylinositol-specific phospholipase C is activated. © 1985.

Registro:

Documento: Artículo
Título:Bradykinin stimulates phospholipase C in rat renal medullary slices
Autor:Speziale, N.; Speziale, E.H.; Pasquini, J.M.
Filiación:Centra de Estudios Farmacológicos y de Principles Nalurales (CEFAPRIN), Consejo National de Investigaciones Cientificasy Técnicas de la República Argentina (CONJCET), Serrano 665 / 669, 1414 Buenos AiresArgentina
Departamento de Química Biológica, Facultad de Farmaciay Bioqúimica, UBA y PRINEUQUI (CONICET), Junín 956, 1113 Buenos AiresArgentina
Palabras clave:(Rat kidney medulla); Bradykinin; Diacylglycerol; Phosphatidylinositol; Phospholipase C; bradykinin; bucladesine; enzyme; indometacin; mepacrine; phosphatidylinositol; phospholipase c; radioisotope; animal cell; arachidonic acid c 14; diacylglycerol c 14; dose response; drug comparison; drug elimination; drug inhibition; drug mechanism; drug metabolism; drug monitoring; drug response; kidney; kidney medulla; nonhuman; phosphatidylinositol c 14; priority journal; rat; thin layer chromatography; Animal; Bradykinin; Bucladesine; Diglycerides; Enzyme Activation; Indomethacin; Kidney Medulla; Male; Phosphatidylinositols; Phospholipase C; Phospholipases; Phospholipids; Quinacrine; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't
Año:1985
Volumen:836
Número:1
Página de inicio:14
Página de fin:18
DOI: http://dx.doi.org/10.1016/0005-2760(85)90214-0
Título revista:Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Título revista abreviado:Biochim. Biophys. Acta Lipids Lipid Metab.
ISSN:00052760
CODEN:BBLLA
CAS:bradykinin, 58-82-2, 5979-11-3; bucladesine, 16980-89-5, 362-74-3; indometacin, 53-86-1, 74252-25-8, 7681-54-1; mepacrine, 69-05-6, 83-89-6; phospholipase C, 9001-86-9; Bradykinin, 58-82-2; Bucladesine, 362-74-3; Diglycerides; Indomethacin, 53-86-1; Phosphatidylinositols; Phospholipase C, EC 3.1.4.3; Phospholipases, EC 3.1.-; Phospholipids; Quinacrine, 83-89-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v836_n1_p14_Speziale

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Citas:

---------- APA ----------
Speziale, N., Speziale, E.H. & Pasquini, J.M. (1985) . Bradykinin stimulates phospholipase C in rat renal medullary slices. Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, 836(1), 14-18.
http://dx.doi.org/10.1016/0005-2760(85)90214-0
---------- CHICAGO ----------
Speziale, N., Speziale, E.H., Pasquini, J.M. "Bradykinin stimulates phospholipase C in rat renal medullary slices" . Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 836, no. 1 (1985) : 14-18.
http://dx.doi.org/10.1016/0005-2760(85)90214-0
---------- MLA ----------
Speziale, N., Speziale, E.H., Pasquini, J.M. "Bradykinin stimulates phospholipase C in rat renal medullary slices" . Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, vol. 836, no. 1, 1985, pp. 14-18.
http://dx.doi.org/10.1016/0005-2760(85)90214-0
---------- VANCOUVER ----------
Speziale, N., Speziale, E.H., Pasquini, J.M. Bradykinin stimulates phospholipase C in rat renal medullary slices. Biochim. Biophys. Acta Lipids Lipid Metab. 1985;836(1):14-18.
http://dx.doi.org/10.1016/0005-2760(85)90214-0