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Abstract:

A system was developed for testing the effect of phospholipids on dolichyl phosphate phosphatase, a membrane-associated enzyme. This enzyme was solubilized, delipidated, stabilized and concentrated in such a way that minimal quantities of Triton X-100 were carried by enzyme extracts to the incubation mixture. Its substrate, dolichyl phosphate, could be kept in aqueous medium as suspended particles without addition of detergent. When dolichyl phosphate phosphatase was assayed using the substrate in this detergent-free form, values for Km, pH optimum and temperature optimum were different from those obtained with detergentsolubilized substrate. This assay of dolichyl phosphate phosphatase almost free of detergent allowed testing of the effect of specific phospholipids on enzyme activity with minimal interference produced by endogenous phospholipids or exogenous detergent. Sphingomyelin, phosphatidylethanolamine or phosphatidylcholine (zwitterionic phospholipids) acted as activators, whereas phosphatidic acid and phosphatidylinositol, negatively-charged phospholipids, were inhibitors of dolichyl phosphate phosphatase. © 1984.

Registro:

Documento: Artículo
Título:Dolichyl phosphate phosphatase in rat liver microsomes. Avoidance of the use of detergent in testing the effect of phospholipids on dolichyl phosphate phosphatase
Autor:Boscoboinik, D.O.; Morera, S.; Belocopitow, E.
Filiación:Instituto de Investigaciones Bioquimicas 'Fundacion Campomar' and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos AiresArgentina
Palabras clave:(Rat liver microsome); Detergent; Dolichyl phosphate phosphatase; Enzyme assay; Phospholipid; phospholipid; animal cell; dolichyl phosphatase; enzyme assay; liver; microsome; nonhuman; rat; Animal; Catalase; Cytochrome c; Detergents; Intracellular Membranes; Kinetics; Microsomes, Liver; Octoxynol; Phospholipids; Phosphoric Monoester Hydrolases; Polyethylene Glycols; Rats; Support, Non-U.S. Gov't; Surface-Active Agents
Año:1984
Volumen:794
Número:1
Página de inicio:41
Página de fin:48
DOI: http://dx.doi.org/10.1016/0005-2760(84)90295-9
Título revista:Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Título revista abreviado:Biochim. Biophys. Acta Lipids Lipid Metab.
ISSN:00052760
CODEN:BBLLA
CAS:Catalase, EC 1.11.1.6; Cytochrome c, 9007-43-6; Detergents; dolichyl-phosphatase, EC 3.1.3.51; Octoxynol, 9002-93-1; Phospholipids; Phosphoric Monoester Hydrolases, EC 3.1.3; Polyethylene Glycols; Surface-Active Agents
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v794_n1_p41_Boscoboinik

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Citas:

---------- APA ----------
Boscoboinik, D.O., Morera, S. & Belocopitow, E. (1984) . Dolichyl phosphate phosphatase in rat liver microsomes. Avoidance of the use of detergent in testing the effect of phospholipids on dolichyl phosphate phosphatase. Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, 794(1), 41-48.
http://dx.doi.org/10.1016/0005-2760(84)90295-9
---------- CHICAGO ----------
Boscoboinik, D.O., Morera, S., Belocopitow, E. "Dolichyl phosphate phosphatase in rat liver microsomes. Avoidance of the use of detergent in testing the effect of phospholipids on dolichyl phosphate phosphatase" . Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 794, no. 1 (1984) : 41-48.
http://dx.doi.org/10.1016/0005-2760(84)90295-9
---------- MLA ----------
Boscoboinik, D.O., Morera, S., Belocopitow, E. "Dolichyl phosphate phosphatase in rat liver microsomes. Avoidance of the use of detergent in testing the effect of phospholipids on dolichyl phosphate phosphatase" . Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, vol. 794, no. 1, 1984, pp. 41-48.
http://dx.doi.org/10.1016/0005-2760(84)90295-9
---------- VANCOUVER ----------
Boscoboinik, D.O., Morera, S., Belocopitow, E. Dolichyl phosphate phosphatase in rat liver microsomes. Avoidance of the use of detergent in testing the effect of phospholipids on dolichyl phosphate phosphatase. Biochim. Biophys. Acta Lipids Lipid Metab. 1984;794(1):41-48.
http://dx.doi.org/10.1016/0005-2760(84)90295-9