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Uhrig, M.L.; Couto, A.S.; Colli, W.; De Lederkremer, R.M. "Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms" (1996) Biochimica et Biophysica Acta - Lipids and Lipid Metabolism. 1300(3):233-239
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Abstract:

In vivo labeling experiments with [3H]palmitic acid, [3H]inositol, and [3H]glucose allowed the identification of two main classes of inositolphospholipids (IPLs) from the trypomastigote stage of Trypanosoma cruzi. Purification of these compounds was achieved by ion-exchange chromatography, high performance liquid chromatography and thin layer chromatography. Specific phosphatidyl-inositol phospholipase C digestion, dephosphorylation and acid methanolysis showed a ceramide structure for the lower migrating IPL1. Palmitoyldihydrosphingosine and palmitoylsphingosine were detected by reverse-phase thin-layer chromatography. On the other hand, IPL2 showed to be a mixture of diacylglycero- and alkylacylglycero-phospholipids in a 1:1 ratio. After PI-PLC digestion, the lipids were separated by preparative TLC and individually analysed. The diacylglycerol contained mainly C18:0 fatty acid together with a low amount of C16:0. Hexadecylglycerol esterified with the C18:0 fatty acid was the only alkylacylglycerol detected. The C18:2 and C18:1 fatty acids, preponderant in the PI molecules of epimastigote forms, were not detected in trypomastigote forms. This is the first report on inositol phospholipids, putative precursors of lipid anchors in the infective stage of T. cruzi.

Registro:

Documento: Artículo
Título:Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms
Autor:Uhrig, M.L.; Couto, A.S.; Colli, W.; De Lederkremer, R.M.
Filiación:Depto. de Quim. Orgánica, Universidad de Buenos Aires, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil
Palabras clave:Inositolphospholipid; T. cruzi; Trypomastigote; inositol derivative; phospholipid; ceramide; diacylglycerol; fatty acid; glycerylphosphoinositol; glycosylphosphatidylinositol; inositol phosphate; palmitic acid; palmitic acid derivative; phosphatidylinositol; sphingosine; article; high performance liquid chromatography; ion exchange chromatography; isotope labeling; life cycle; lipid analysis; priority journal; thin layer chromatography; Trypanosoma cruzi; animal; chemical structure; chemistry; isolation and purification; metabolism; Trypanosoma; Trypanosoma cruzi; Animals; Ceramides; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Diglycerides; Fatty Acids; Glycosylphosphatidylinositols; Inositol Phosphates; Molecular Structure; Palmitic Acid; Palmitic Acids; Phosphatidylinositols; Sphingosine; Trypanosoma cruzi
Año:1996
Volumen:1300
Número:3
Página de inicio:233
Página de fin:239
DOI: http://dx.doi.org/10.1016/0005-2760(96)00021-5
Título revista:Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
Título revista abreviado:BIOCHIM. BIOPHYS. ACTA LIPIDS LIPID METAB.
ISSN:00052760
CODEN:BBLLA
CAS:Ceramides; Diglycerides; Fatty Acids; glycerylphosphoinositol, 16824-65-0; Glycosylphosphatidylinositols; Inositol Phosphates; Palmitic Acid, 57-10-3; Palmitic Acids; Phosphatidylinositols; Sphingosine, 123-78-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v1300_n3_p233_Uhrig

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Citas:

---------- APA ----------
Uhrig, M.L., Couto, A.S., Colli, W. & De Lederkremer, R.M. (1996) . Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms. Biochimica et Biophysica Acta - Lipids and Lipid Metabolism, 1300(3), 233-239.
http://dx.doi.org/10.1016/0005-2760(96)00021-5
---------- CHICAGO ----------
Uhrig, M.L., Couto, A.S., Colli, W., De Lederkremer, R.M. "Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms" . Biochimica et Biophysica Acta - Lipids and Lipid Metabolism 1300, no. 3 (1996) : 233-239.
http://dx.doi.org/10.1016/0005-2760(96)00021-5
---------- MLA ----------
Uhrig, M.L., Couto, A.S., Colli, W., De Lederkremer, R.M. "Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms" . Biochimica et Biophysica Acta - Lipids and Lipid Metabolism, vol. 1300, no. 3, 1996, pp. 233-239.
http://dx.doi.org/10.1016/0005-2760(96)00021-5
---------- VANCOUVER ----------
Uhrig, M.L., Couto, A.S., Colli, W., De Lederkremer, R.M. Characterization of inositolphospholipids in Tryparzosoma cruzi trypmastigote forms. BIOCHIM. BIOPHYS. ACTA LIPIDS LIPID METAB. 1996;1300(3):233-239.
http://dx.doi.org/10.1016/0005-2760(96)00021-5