Artículo
Abstract:
Soybean callus succinyl CoA synthetase (succinate : CoA ligase, (ADP-forming), EC 6.2.1.5), has been chemically bound to Sepharose 4B and some of its properties have been studied. The optimal conditions for binding have been determined. The immobilized enzyme retained 48% of the activity of the soluble enzyme and the coupling yield amounted to 50%. Sepharose · succinyl CoA synthetase can be stored at 4°C for periods up to 90 days with only 25% loss of activity; it can also be repeatedly used without alteration of its enzymic activity. The complex showed enhanced thermal stability; pH optimum was between 7.0 and 8.0 for the bound enzyme, and 8.0 for the free enzyme. A general decrease in the Michaelis-Menten constants for the different substrates of the insoluble enzyme, as compared with values obtained for the free enzyme, was found. Plots of the rate product formation against ATP concentration changed from sigmoideal for the soluble succinyl CoA synthetase to hyperbolic for the immobilized enzyme. © 1978.
Registro:
| Documento: | Artículo |
| Título: | Porphyrin biosynthesis: Immobilized enzymes and ligands VI. Studies on succinyl CoA synthetase from cultured soya bean cells |
| Autor: | Wider de Xifra, E.A.; Batlle, A.M.D.C. |
| Filiación: | Laboratorio de Porfirinas, Departamento de Química Biológica. Facultad de Ciencias Exactas y Naturales, U.B.A. Ciudad Universitaria, 1428 Nuñez Buenos Aires, Argentina |
| Palabras clave: | enzyme; porphyrin; succinyl coenzyme a synthetase; animal experiment; higher plant; in vitro study; Cells, Cultured; Coenzyme A Ligases; Drug Stability; Enzymes, Immobilized; Kinetics; Plants; Porphyrins; Soybeans; Succinate-CoA Ligases |
| Año: | 1978 |
| Volumen: | 523 |
| Número: | 1 |
| Página de inicio: | 245 |
| Página de fin: | 249 |
| DOI: | http://dx.doi.org/10.1016/0005-2744(78)90027-X |
| Título revista: | BBA - Enzymology |
| ISSN: | 00052744 |
| CAS: | porphyrin, 24869-67-8; succinyl coenzyme A synthetase, 37341-57-4, 9014-36-2, 9080-33-5; Coenzyme A Ligases, EC 6.2.1.; Enzymes, Immobilized; Porphyrins; Succinate-CoA Ligases, EC 6.2.1.- |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v523_n1_p245_WiderdeXifra |
Referencias:
- Batlle A.M. del, Rossetti, (1977) Int. J. Biochem., 8, pp. 251-267
- Stella, Wider de Xifra, Batlle A.M. del, (1977) Mol. Cell. Biochem., 16, pp. 97-106
- Wider de Xifra, Stella, Batlle A.M. del, Plant Sci Lett. (1977) Plant Sci Lett., , in the press
- Stella, Batlle A.M. del, (1977) Plant Sci. Lett., , in the press
- Rossetti, Parera, Batlle A.M. del, (1977) Act. Phys. Lat., , in the press
- Wider de Xifra, Mendiara, Batlle A.M. del, (1972) FEBS Lett., 27, pp. 275-278
- Wider de Xifra, Batlle A.M. del, (1973) Enzyme, 16, pp. 128-137
- Wider de Xifra, Batlle A.M. del, (1976) Porphyrin in Human Diseases, pp. 25-34. , M. Doss, Karger, Basel
- Wider de Xifra, Tigier, (1970) FEBS Lett., 9, pp. 30-32
- Wider de Xifra, Tigier, (1971) Enzymology, 41, pp. 217-231
- Wider de Xifra, Batlle A.M. del, (1974) Int. J. Biochem., 5, pp. 129-135
- Batlle A.M. del, Llambias, Wider de Xifra, Tigier, (1975) Int. J. Biochem., 6, pp. 591-606
- Crook, (1969) FEBS Lett., 19, pp. 297-308
Citas:
---------- APA ----------
Wider de Xifra, E.A. & Batlle, A.M.D.C. (1978) . Porphyrin biosynthesis: Immobilized enzymes and ligands VI. Studies on succinyl CoA synthetase from cultured soya bean cells. BBA - Enzymology, 523(1), 245-249.http://dx.doi.org/10.1016/0005-2744(78)90027-X
---------- CHICAGO ----------
Wider de Xifra, E.A., Batlle, A.M.D.C. "Porphyrin biosynthesis: Immobilized enzymes and ligands VI. Studies on succinyl CoA synthetase from cultured soya bean cells" . BBA - Enzymology 523, no. 1 (1978) : 245-249.http://dx.doi.org/10.1016/0005-2744(78)90027-X
---------- MLA ----------
Wider de Xifra, E.A., Batlle, A.M.D.C. "Porphyrin biosynthesis: Immobilized enzymes and ligands VI. Studies on succinyl CoA synthetase from cultured soya bean cells" . BBA - Enzymology, vol. 523, no. 1, 1978, pp. 245-249.http://dx.doi.org/10.1016/0005-2744(78)90027-X
---------- VANCOUVER ----------
Wider de Xifra, E.A., Batlle, A.M.D.C. Porphyrin biosynthesis: Immobilized enzymes and ligands VI. Studies on succinyl CoA synthetase from cultured soya bean cells. 1978;523(1):245-249.http://dx.doi.org/10.1016/0005-2744(78)90027-X
