1. 1. δ-Aminolaevulinate synthetase has been detected in extracts of soybean callus tissues and the enzyme activity reached its maximum when callus were 11 days old. 2. 2. The presence of a compound which seems to control δ-aminolaevulinate synthetase activity was demonstrated. The enzyme was present in the soluble fraction and was very labile. 3. 3. When crude extracts or 500 × g supernatant were stored at 4-6°, the apparent activity of δ-aminolaevulinate synthetase increased by as much as 3-6 times, while the activities of δ-aminolaevulinate dehydratase and succinyl-CoA synthetase did not significantly change during the storage. Activation was dependent on concentrations of cells suspensions during disruption and aging. 4. 4. Gel filtration with Sephadex G-25 of 2000 × g supernatants produced an enzyme fraction 30% more active. An increase in enzyme activity was observed when dark-grown callus were exposed to light. 5. 5. The addition of ATP, gibberellic acid and δ-aminolaevulinate to the culture media diminished activity; iron deficiency also produced an δ-aminolaevulinate synthetase less active. © 1971.
| Documento: | Artículo |
| Título: | δ-aminolaevulinate synthetase in extracts of cultured soybean cells |
| Autor: | De Xifra, E.A.W.; Del C. Batlle, A.M.; Tigier, H.A. |
| Filiación: | Cátedra de Química Biológica I, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Perú, 272 Buenos Aires, Argentina |
| Año: | 1971 |
| Volumen: | 235 |
| Número: | 3 |
| Página de inicio: | 511 |
| Página de fin: | 517 |
| DOI: | http://dx.doi.org/10.1016/0005-2744(71)90292-0 |
| Título revista: | BBA - Enzymology |
| ISSN: | 00052744 |
| PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00052744_v235_n3_p511_DeXifra.pdf |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v235_n3_p511_DeXifra |
