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Abstract:

1. 1. δ-Aminolaevulinate synthetase has been detected in extracts of soybean callus tissues and the enzyme activity reached its maximum when callus were 11 days old. 2. 2. The presence of a compound which seems to control δ-aminolaevulinate synthetase activity was demonstrated. The enzyme was present in the soluble fraction and was very labile. 3. 3. When crude extracts or 500 × g supernatant were stored at 4-6°, the apparent activity of δ-aminolaevulinate synthetase increased by as much as 3-6 times, while the activities of δ-aminolaevulinate dehydratase and succinyl-CoA synthetase did not significantly change during the storage. Activation was dependent on concentrations of cells suspensions during disruption and aging. 4. 4. Gel filtration with Sephadex G-25 of 2000 × g supernatants produced an enzyme fraction 30% more active. An increase in enzyme activity was observed when dark-grown callus were exposed to light. 5. 5. The addition of ATP, gibberellic acid and δ-aminolaevulinate to the culture media diminished activity; iron deficiency also produced an δ-aminolaevulinate synthetase less active. © 1971.

Registro:

Documento: Artículo
Título:δ-aminolaevulinate synthetase in extracts of cultured soybean cells
Autor:De Xifra, E.A.W.; Del C. Batlle, A.M.; Tigier, H.A.
Filiación:Cátedra de Química Biológica I, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Perú, 272 Buenos Aires, Argentina
Año:1971
Volumen:235
Número:3
Página de inicio:511
Página de fin:517
DOI: http://dx.doi.org/10.1016/0005-2744(71)90292-0
Título revista:BBA - Enzymology
ISSN:00052744
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00052744_v235_n3_p511_DeXifra.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v235_n3_p511_DeXifra

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Citas:

---------- APA ----------
De Xifra, E.A.W., Del C. Batlle, A.M. & Tigier, H.A. (1971) . δ-aminolaevulinate synthetase in extracts of cultured soybean cells. BBA - Enzymology, 235(3), 511-517.
http://dx.doi.org/10.1016/0005-2744(71)90292-0
---------- CHICAGO ----------
De Xifra, E.A.W., Del C. Batlle, A.M., Tigier, H.A. "δ-aminolaevulinate synthetase in extracts of cultured soybean cells" . BBA - Enzymology 235, no. 3 (1971) : 511-517.
http://dx.doi.org/10.1016/0005-2744(71)90292-0
---------- MLA ----------
De Xifra, E.A.W., Del C. Batlle, A.M., Tigier, H.A. "δ-aminolaevulinate synthetase in extracts of cultured soybean cells" . BBA - Enzymology, vol. 235, no. 3, 1971, pp. 511-517.
http://dx.doi.org/10.1016/0005-2744(71)90292-0
---------- VANCOUVER ----------
De Xifra, E.A.W., Del C. Batlle, A.M., Tigier, H.A. δ-aminolaevulinate synthetase in extracts of cultured soybean cells. 1971;235(3):511-517.
http://dx.doi.org/10.1016/0005-2744(71)90292-0