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Abstract:

Fructokinase (ATP:d-fructose-1-phosphate transferase, EC 2.7.1.3) from rat liver has been purified 400-fold. The purification procedure involves an acid treatment, a heat step at 65°, (NH4)2SO4 fractionation, chromatography on Sephadex G-100 and finally (NH4)2SO4 extraction. The enzyme appears nearly homogenous by density gradient centrifugation but gives a single peak in sedimentation velocity analysis. Purified liver fructokinase has a Km of 0.46-0.80 mM for fructose and 1.56-1.33 mM for MgATP at a K+ concentration of 0.4 and 0.1 M, respectively. The enzyme also phosphorylates l-sorbose and d-tagatose. No difference could be found in the phosphorylation of the pyranose and furanose forms of fructose. The enzyme is inhibited by p-chloromercuribenzoate and is stable up to 50-55°. © 1971.

Registro:

Documento: Artículo
Título:Fructokinase from rat liver. I. Purification and properties
Autor:Sánchez, J.J.; González, N.S.; Pontis, H.G.
Filiación:Instituto de Investigaciones Bioquímicas, Fundación Campomar, Obligado, 2490 Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Buenos Aires (28), Obligado 2490, Argentina
Idioma: Inglés
Palabras clave:4 chloromercuribenzoic acid; acrylic acid derivative; adenine nucleotide; ammonium derivative; absorption; animal; article; biosynthesis; colorimetry; density gradient centrifugation; Absorption; Acrylates; Adenine Nucleotides; Ammonium Compounds; Animal; Centrifugation, Density Gradient; Chloromercuribenzoates; Colorimetry
Año:1971
Volumen:227
Número:1
Página de inicio:67
Página de fin:78
Título revista:BBA - Enzymology
ISSN:00052744
CAS:4 chloromercuribenzoic acid, 59-85-8; Acrylates; Adenine Nucleotides; Ammonium Compounds; Chloromercuribenzoates
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00052744_v227_n1_p67_Sanchez.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v227_n1_p67_Sanchez

Citas:

---------- APA ----------
Sánchez, J.J., González, N.S. & Pontis, H.G. (1971) . Fructokinase from rat liver. I. Purification and properties. BBA - Enzymology, 227(1), 67-78.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v227_n1_p67_Sanchez [ ]
---------- CHICAGO ----------
Sánchez, J.J., González, N.S., Pontis, H.G. "Fructokinase from rat liver. I. Purification and properties" . BBA - Enzymology 227, no. 1 (1971) : 67-78.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v227_n1_p67_Sanchez [ ]
---------- MLA ----------
Sánchez, J.J., González, N.S., Pontis, H.G. "Fructokinase from rat liver. I. Purification and properties" . BBA - Enzymology, vol. 227, no. 1, 1971, pp. 67-78.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v227_n1_p67_Sanchez [ ]
---------- VANCOUVER ----------
Sánchez, J.J., González, N.S., Pontis, H.G. Fructokinase from rat liver. I. Purification and properties. 1971;227(1):67-78.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v227_n1_p67_Sanchez [ ]