Fructokinase (ATP:d-fructose-1-phosphate transferase, EC 2.7.1.3) from rat liver has been purified 400-fold. The purification procedure involves an acid treatment, a heat step at 65°, (NH4)2SO4 fractionation, chromatography on Sephadex G-100 and finally (NH4)2SO4 extraction. The enzyme appears nearly homogenous by density gradient centrifugation but gives a single peak in sedimentation velocity analysis. Purified liver fructokinase has a Km of 0.46-0.80 mM for fructose and 1.56-1.33 mM for MgATP at a K+ concentration of 0.4 and 0.1 M, respectively. The enzyme also phosphorylates l-sorbose and d-tagatose. No difference could be found in the phosphorylation of the pyranose and furanose forms of fructose. The enzyme is inhibited by p-chloromercuribenzoate and is stable up to 50-55°. © 1971.
Documento: | Artículo |
Título: | Fructokinase from rat liver. I. Purification and properties |
Autor: | Sánchez, J.J.; González, N.S.; Pontis, H.G. |
Filiación: | Instituto de Investigaciones Bioquímicas, Fundación Campomar, Obligado, 2490 Buenos Aires, Argentina Facultad de Ciencias Exactas y Naturales, Buenos Aires (28), Obligado 2490, Argentina |
Idioma: | Inglés |
Palabras clave: | 4 chloromercuribenzoic acid; acrylic acid derivative; adenine nucleotide; ammonium derivative; absorption; animal; article; biosynthesis; colorimetry; density gradient centrifugation; Absorption; Acrylates; Adenine Nucleotides; Ammonium Compounds; Animal; Centrifugation, Density Gradient; Chloromercuribenzoates; Colorimetry |
Año: | 1971 |
Volumen: | 227 |
Número: | 1 |
Página de inicio: | 67 |
Página de fin: | 78 |
Título revista: | BBA - Enzymology |
ISSN: | 00052744 |
CAS: | 4 chloromercuribenzoic acid, 59-85-8; Acrylates; Adenine Nucleotides; Ammonium Compounds; Chloromercuribenzoates |
PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00052744_v227_n1_p67_Sanchez.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v227_n1_p67_Sanchez |