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Abstract:

1. 1. A method for the isolation and purification of porphobilinogenase, porphobilinogen deaminase and uroporphyrinogen isomerase from avian erythrocytes is described. 2. 2. Some properties of the isolated enzymes were studied. The optimal pH for porphobilinogenase and deaminase was 7.4. Purified porphobilinogenase was resolved into three bands on starch gel electrophoresis. The molecular weight of the purified enzymes was determined by gel filtration. The presence of porphobilinogen or NH4 + at certain concentrations afforded protection against heat inactivation of isomerase, the heat labile enzyme. Initial porphyrin formation by porphobilinogenase was linear with time. 3. 3. The action of various compounds added to the system was studied. Thiol reagents inhibited both porphobilinogenase and deaminase, indicating the presence of thiol groups essential for activity. NH4 +, hydroxylamine, adenine, ADP, ATP, some dicarboxylic acids and 2-methoxy-5-nitrotropone inhibited deaminase. © 1971.

Registro:

Documento: Artículo
Título:Porphyrin biosynthesis. VIII. Avian erythrocyte porphobilinogen deaminase-uroporphyrinogen III cosynthetase, its purification, properties and the separation of its components
Autor:Llambías, E.B.C.; Del C. Batlle, A.M.
Filiación:Cátedra de Química Biológica I, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Perú, 272 Buenos Aires, Argentina
Palabras clave:4 chloromercuribenzoic acid; adenine nucleotide; adenosine triphosphate; ammonia; ammonium derivative; Carboxy Lyases; carboxylyase; cysteine; dicarboxylic acid; animal; article; chicken; dialysis; ion exchange chromatography; isolation and purification; metabolism; Adenine Nucleotides; Adenosine Triphosphate; Ammonia; Ammonium Compounds; Animal; Carboxy-Lyases; Chickens; Chloromercuribenzoates; Chromatography, DEAE-Cellulose; Cysteine; Dialysis; Dicarboxylic Acids
Año:1971
Volumen:227
Número:1
Página de inicio:180
Página de fin:191
DOI: http://dx.doi.org/10.1016/0005-2744(71)90178-1
Título revista:BBA - Enzymology
ISSN:00052744
CAS:4 chloromercuribenzoic acid, 59-85-8; adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; carboxylyase, 9027-22-9; cysteine, 4371-52-2, 52-89-1, 52-90-4; Adenine Nucleotides; Adenosine Triphosphate, 56-65-5; Ammonia, 7664-41-7; Ammonium Compounds; Carboxy-Lyases, EC 4.1.1.; Chloromercuribenzoates; Cysteine, 52-90-4; Dicarboxylic Acids
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00052744_v227_n1_p180_Llambias.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v227_n1_p180_Llambias

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Citas:

---------- APA ----------
Llambías, E.B.C. & Del C. Batlle, A.M. (1971) . Porphyrin biosynthesis. VIII. Avian erythrocyte porphobilinogen deaminase-uroporphyrinogen III cosynthetase, its purification, properties and the separation of its components. BBA - Enzymology, 227(1), 180-191.
http://dx.doi.org/10.1016/0005-2744(71)90178-1
---------- CHICAGO ----------
Llambías, E.B.C., Del C. Batlle, A.M. "Porphyrin biosynthesis. VIII. Avian erythrocyte porphobilinogen deaminase-uroporphyrinogen III cosynthetase, its purification, properties and the separation of its components" . BBA - Enzymology 227, no. 1 (1971) : 180-191.
http://dx.doi.org/10.1016/0005-2744(71)90178-1
---------- MLA ----------
Llambías, E.B.C., Del C. Batlle, A.M. "Porphyrin biosynthesis. VIII. Avian erythrocyte porphobilinogen deaminase-uroporphyrinogen III cosynthetase, its purification, properties and the separation of its components" . BBA - Enzymology, vol. 227, no. 1, 1971, pp. 180-191.
http://dx.doi.org/10.1016/0005-2744(71)90178-1
---------- VANCOUVER ----------
Llambías, E.B.C., Del C. Batlle, A.M. Porphyrin biosynthesis. VIII. Avian erythrocyte porphobilinogen deaminase-uroporphyrinogen III cosynthetase, its purification, properties and the separation of its components. 1971;227(1):180-191.
http://dx.doi.org/10.1016/0005-2744(71)90178-1