Artículo

Ohtake, S.; Schebor, C.; de Pablo, J.J. "Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles" (2006) Biochimica et Biophysica Acta - Biomembranes. 1758(1):65-73
Artículo de Acceso Abierto. Puede ser descargado en su versión final
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

A systematic study is presented of the effects of trehalose on the physical properties of extruded DPPC-cholesterol unilamellar vesicles. Particular emphasis is placed on examining how the interactions present in the hydrated state translate into those in the dehydrated state. Observations from HSDSC and DSC are used to examine the phase behavior of hydrated and dehydrated vesicles, respectively. The concentration of trehalose inside and outside the vesicles is manipulated, and is shown to affect the relative stability of the membranes. Our results show for the first time that a combination of high inner and low outer trehalose concentration is able to decrease the gel-to-liquid crystalline phase temperature (Tm), while any other combination will not. Upon dehydration, the Tm of all lipid mixtures increases. The extent of the increase depends on the trehalose distribution across the bilayer. The Tm changes in the same direction with trehalose concentration for both freeze-dried and fully hydrated samples, suggesting that the trehalose distribution across the vesicle membrane, as well as the trehalose-phospholipid interaction, is maintained upon lyophilization. The results presented in this work may aid in the formulation of systems to be used in the lyophilization of liposomes for drug delivery applications. © 2006 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles
Autor:Ohtake, S.; Schebor, C.; de Pablo, J.J.
Filiación:Chemical and Biological Engineering Department, University of Wisconsin, 1415 Engineering Drive, Madison, WI 53706, United States
Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Ciudad Autonoma de Buenos Aires, Argentina
Palabras clave:Cholesterol; DPPC; Liposome; Trehalose; cholesterol; crystallin; dipalmitoylphosphatidylcholine; liposome; phospholipid; trehalose; article; concentration (parameters); dehydration; drug delivery system; freeze drying; gel; hydration; liquid; priority journal; protein interaction; temperature; 1,2-Dipalmitoylphosphatidylcholine; Calorimetry, Differential Scanning; Cell Membrane; Cholesterol; Drug Delivery Systems; Freeze Drying; Lipid Bilayers; Phase Transition; Temperature; Trehalose; Water
Año:2006
Volumen:1758
Número:1
Página de inicio:65
Página de fin:73
DOI: http://dx.doi.org/10.1016/j.bbamem.2006.01.002
Handle:http://hdl.handle.net/20.500.12110/paper_00052736_v1758_n1_p65_Ohtake
Título revista:Biochimica et Biophysica Acta - Biomembranes
Título revista abreviado:Biochim. Biophys. Acta Biomembr.
ISSN:00052736
CODEN:BBBMB
CAS:cholesterol, 57-88-5; crystallin, 11046-99-4; dipalmitoylphosphatidylcholine, 2644-64-6; trehalose, 99-20-7; 1,2-Dipalmitoylphosphatidylcholine, 2644-64-6; Cholesterol, 57-88-5; Lipid Bilayers; Trehalose, 99-20-7; Water, 7732-18-5
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00052736_v1758_n1_p65_Ohtake.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052736_v1758_n1_p65_Ohtake

Referencias:

  • Timasheff, S.N., Preferential interactions of water and cosolvents with proteins (1995) Protein-Solvent Interactions, pp. 445-482. , Gregory R. (Ed), Dekker, New York
  • Crowe, J.H., Crowe, L.M., Chapman, D., Preservation of membranes in anhydrobiotic organisms: the role of trehalose (1984) Science, 223, pp. 701-703
  • Crowe, J., Hoekstra, F., Nguyen, K., Crowe, L., Is vitrification involved in depression of the phase transition temperature in dry phospholipids? (1996) Biochim. Biophys. Acta, 1280, pp. 187-196
  • Koster, K., lei, Y., Anderson, M., Martin, S., Bryant, G., Effects of vitrified and nonvitrified sugars on phosphatidylcholine fluid-to-gel phase transition (2000) Biophys. J., 78, pp. 1932-1946
  • Crowe, J.H., Whittam, M.S., Chapman, D., Crowe, L.M., Interactions of phospholipid monolayers with carbohydrates (1984) Biochim. Biophys. Acta, 769, pp. 151-159
  • Johnston, D.S., Coppard, E., Parera, G.V., Chapman, D., Langmuir film balance study of the interactions between carbohydrates and phospholipid monolayers (1984) Biochemistry, 23, pp. 6912-6919
  • Lambruschini, C., Relini, A., Cordone, L., Gliozzi, A., Trehalose interacts with phospholipid polar heads in Langmuir monolayers (2000) Langmuir, 16, pp. 5467-5470
  • Luzardo, M., Amalfa, F., Nuñez, A.M., Biondi de López, A.C., Disalvo, E.A., Effect of trehalose and sucrose on the hydration and dipole potential of lipid bilayers (2000) Biophys. J., 78, pp. 2452-2458
  • Sum, A., Faller, R., de Pablo, J.J., Molecular simulation study of phospholipid bilayers and insights of the interactions with disaccharides (2003) Biophys. J., 85, pp. 2830-2844
  • Doxastakis, M., Sum, A.K., de Pablo, J.J., Modulating membrane properties: the effect of trehalose and cholesterol on a phospholipid bilayers (2005) J. Phys. Chem., B, 109, pp. 24173-24181
  • Pereira, C.S., Lins, R.D., Chandrasekhar, I., Freitas, L.C.G., Hünenberger, P.H., Interaction of the disaccharide trehalose with a phospholipid bilayer: A molecular dynamics study (2004) Biophys. J., 86, pp. 2273-2285
  • Villarreal, M.A., Diaz, S.B., Disalvo, E.A., Montich, G.G., Molecular dynamics simulation study of the interaction of trehalose with lipid membranes (2004) Langmuir, 20, pp. 7844-7851
  • Nishiwaki, T., Sakurai, M., Inoue, Y., Chujo, R., Kobayashi, S., Increasing packing density of hydrated dipalmitoylphosphatidylcholine unilamellar vesicles induced by trehalose (1990) Chem. Lett., pp. 1841-1844
  • Crowe, L.M., Crowe, J.H., Solution effects on the thermotropic phase transition of unilamellar liposomes (1991) Biochim. Biophys. Acta, 1064, pp. 267-274
  • Tsvetkov, T.D., Tsonev, L.I., Tsvetkova, N.M., Koynova, R.D., Tenchov, B.G., Effect of trehalose on the phase properties of hydrated and lyophilized dipalmitoylphosphatidylcholine multilayers (1989) Criobiology, 26, pp. 162-169
  • Cevc, G., Effect of lipid headgroups and (nonelectrolyte) solution on the structural and phase properties of bilayer membranes (1988) Ber. Bunsenges. Phys. Chem., 92, pp. 953-961
  • Koynova, R.D., Tenchov, B.G., Quinn, P.J., Sugars favour formation of hexagonal (HII) phase at the expense of lamellar liquid-crystalline phase in hydrated phosphatidylethanolamines (1989) Biochim. Biophys. Acta, 980, pp. 377-380
  • Hays, L.M., Crowe, J.H., Wolkers, W., Rudenko, W.S., Factors affecting leakage of trapped solutes from phospholipid vesicles during thermotropic phase transitions (2001) Cryobiology, 42, pp. 88-102
  • Ricker, J., Tsvetkova, N., Wolkers, W., Leidy, C., Tablin, F., Longo, M., Crowe, J.H., Trehalose maintains phase separation in an air-dried binary lipid mixture (2003) Biophys. J., 84, pp. 3045-3051
  • Ohtake, S., Schebor, C., Palecek, S.P., de Pablo, J.J., Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose (2005) Biochim. Biophys. Acta, 1713, pp. 57-64
  • Crowe, L.M., Crowe, J.H., Trehalose and dry dipalmitoylphosphatidylcholine revisited (1988) Biochim. Biophys. Acta, 946, pp. 193-201
  • Nagase, H., Ueda, H., Nakagaki, M., Effect of water on lamellar structure of DPPC/sugar systems (1997) Biochim. Biophys. Acta, 1328, pp. 197-206
  • Ohtake, S., Schebor, C., Palecek, S., de Pablo, J.J., Effect of sugar-phosphate mixtures on the stability of DPPC membranes in dehydrated systems (2004) Cryobiology, 48, pp. 81-89
  • Zhang, J., Steponkus, P., Proposed mechanism for depression of the liquid-crystalline-to-gel phase transition temperature of phospholipids in dehydrated sugar-phospholipid mixtures (1996) Cryobiology, 33, pp. 21A
  • Rouser, G., Nelson, G., Fleischer, S., Simon, G., (1968) Biological Membranes Physical Fact and Function, pp. 5-69. , Chapman D. (Ed), Academic Press, London
  • Ladbrooke, B.W., Williams, R.M., Chapman, D., Studies on lecithin-cholesterol-water interactions by differential scanning calorimetry and X-ray diffraction (1968) Biochim. Biophys. Acta, 150, pp. 333-340
  • McMullen, T., McElhaney, R., Physical studies of cholesterol-phospholipid interactions (1996) Curr. Opin. Colloid Interface Sci., 1, pp. 83-90
  • Simon, S.A., McIntosh, T.J., Latorre, R., Influence of cholesterol on water penetration into bilayers (1982) Science, 216, pp. 65-67
  • McMullen, T., Lewis, R., McElhaney, R., Differential scanning calorimetric study of the effect of cholesterol on the thermotropic phase behavior of a homologous series of linear saturated phosphatidylcholines (1993) Biochemistry, 32, pp. 516-522
  • McMullen, T., McElhaney, R., Differential scanning calorimetric studies of the interaction of cholesterol with distearoyl and dielaidoyl molecular species of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine (1997) Biochemistry, 36, pp. 4979-4986
  • Harrigan, P., Madden, T., Cullis, P., Protection of liposomes during dehydration or freezing (1990) Chem. Phys. Lipids, 52, pp. 139-149
  • Van Winden, E., Crommelin, D., Short term stability of freeze-dried, lyoprotected liposomes (1999) J. Control. Release, 58, pp. 69-86
  • Hobai, S., Fazakas, Z., An approximate cooperativity analysis of the phase transitions of DPPC-DOCNA dispersions (2002) Cell. Mol. Biol. Lett., 7, p. 230
  • Provencher, S.W., Contin: a general purpose constrained regularization program for inverting noisy linear algebraic and integral equations (1982) Comput. Phys. Commun., 27, pp. 229-242
  • Sheetz, M.P., Chan, S.I., Effect of sonication on the structure of lecithin bilayers (1972) Biochemistry, 11, pp. 4573-4581
  • Bramhall, J., Phospholipid packing asymmetry in curved membranes detected by fluorescence spectroscopy (1986) Biochemistry, 25, pp. 3479-3486
  • Crowe, J.H., Carpenter, J.F., Crowe, L.M., The role of vitrification in anhydrobiosis (1998) Annu. Rev. Physiol., 6, pp. 73-103

Citas:

---------- APA ----------
Ohtake, S., Schebor, C. & de Pablo, J.J. (2006) . Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles. Biochimica et Biophysica Acta - Biomembranes, 1758(1), 65-73.
http://dx.doi.org/10.1016/j.bbamem.2006.01.002
---------- CHICAGO ----------
Ohtake, S., Schebor, C., de Pablo, J.J. "Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles" . Biochimica et Biophysica Acta - Biomembranes 1758, no. 1 (2006) : 65-73.
http://dx.doi.org/10.1016/j.bbamem.2006.01.002
---------- MLA ----------
Ohtake, S., Schebor, C., de Pablo, J.J. "Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles" . Biochimica et Biophysica Acta - Biomembranes, vol. 1758, no. 1, 2006, pp. 65-73.
http://dx.doi.org/10.1016/j.bbamem.2006.01.002
---------- VANCOUVER ----------
Ohtake, S., Schebor, C., de Pablo, J.J. Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles. Biochim. Biophys. Acta Biomembr. 2006;1758(1):65-73.
http://dx.doi.org/10.1016/j.bbamem.2006.01.002