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A systematic study is presented of the effects of trehalose on the physical properties of extruded DPPC-cholesterol unilamellar vesicles. Particular emphasis is placed on examining how the interactions present in the hydrated state translate into those in the dehydrated state. Observations from HSDSC and DSC are used to examine the phase behavior of hydrated and dehydrated vesicles, respectively. The concentration of trehalose inside and outside the vesicles is manipulated, and is shown to affect the relative stability of the membranes. Our results show for the first time that a combination of high inner and low outer trehalose concentration is able to decrease the gel-to-liquid crystalline phase temperature (Tm), while any other combination will not. Upon dehydration, the Tm of all lipid mixtures increases. The extent of the increase depends on the trehalose distribution across the bilayer. The Tm changes in the same direction with trehalose concentration for both freeze-dried and fully hydrated samples, suggesting that the trehalose distribution across the vesicle membrane, as well as the trehalose-phospholipid interaction, is maintained upon lyophilization. The results presented in this work may aid in the formulation of systems to be used in the lyophilization of liposomes for drug delivery applications. © 2006 Elsevier B.V. All rights reserved.


Documento: Artículo
Título:Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles
Autor:Ohtake, S.; Schebor, C.; de Pablo, J.J.
Filiación:Chemical and Biological Engineering Department, University of Wisconsin, 1415 Engineering Drive, Madison, WI 53706, United States
Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Ciudad Autonoma de Buenos Aires, Argentina
Palabras clave:Cholesterol; DPPC; Liposome; Trehalose; cholesterol; crystallin; dipalmitoylphosphatidylcholine; liposome; phospholipid; trehalose; article; concentration (parameters); dehydration; drug delivery system; freeze drying; gel; hydration; liquid; priority journal; protein interaction; temperature; 1,2-Dipalmitoylphosphatidylcholine; Calorimetry, Differential Scanning; Cell Membrane; Cholesterol; Drug Delivery Systems; Freeze Drying; Lipid Bilayers; Phase Transition; Temperature; Trehalose; Water
Página de inicio:65
Página de fin:73
Título revista:Biochimica et Biophysica Acta - Biomembranes
Título revista abreviado:Biochim. Biophys. Acta Biomembr.
CAS:cholesterol, 57-88-5; crystallin, 11046-99-4; dipalmitoylphosphatidylcholine, 2644-64-6; trehalose, 99-20-7; 1,2-Dipalmitoylphosphatidylcholine, 2644-64-6; Cholesterol, 57-88-5; Lipid Bilayers; Trehalose, 99-20-7; Water, 7732-18-5


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---------- APA ----------
Ohtake, S., Schebor, C. & de Pablo, J.J. (2006) . Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles. Biochimica et Biophysica Acta - Biomembranes, 1758(1), 65-73.
---------- CHICAGO ----------
Ohtake, S., Schebor, C., de Pablo, J.J. "Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles" . Biochimica et Biophysica Acta - Biomembranes 1758, no. 1 (2006) : 65-73.
---------- MLA ----------
Ohtake, S., Schebor, C., de Pablo, J.J. "Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles" . Biochimica et Biophysica Acta - Biomembranes, vol. 1758, no. 1, 2006, pp. 65-73.
---------- VANCOUVER ----------
Ohtake, S., Schebor, C., de Pablo, J.J. Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles. Biochim. Biophys. Acta Biomembr. 2006;1758(1):65-73.