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Abstract:

A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
Autor:Ohtake, S.; Schebor, C.; Palecek, S.P.; De Pablo, J.J.
Filiación:Chemical and Biological Engineering Department, University of Wisconsin, 1415 Engineering Drive, Madison, WI 53706, United States
Departamento de Industrias, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (1428) Cd. Auton. de Buenos Aires, Argentina
CONICET, Argentina
Palabras clave:Cholesterol; DPPC; DPPE; Freeze-dried; Phase transition; cholesterol; dipalmitoylphosphatidylcholine; dipalmitoylphosphatidylethanolamine; liposome; phospholipid; trehalose; article; enthalpy; freeze drying; lipid composition; lipid membrane; phase separation; phase transition; priority journal; temperature sensitivity; thermostability; 1,2-Dipalmitoylphosphatidylcholine; Calorimetry, Differential Scanning; Cholesterol; Freeze Drying; Phosphatidylethanolamines; Trehalose; Mammalia
Año:2005
Volumen:1713
Número:1
Página de inicio:57
Página de fin:64
DOI: http://dx.doi.org/10.1016/j.bbamem.2005.05.001
Título revista:Biochimica et Biophysica Acta - Biomembranes
Título revista abreviado:Biochim. Biophys. Acta Biomembr.
ISSN:00052736
CODEN:BBBMB
CAS:cholesterol, 57-88-5; dipalmitoylphosphatidylcholine, 2644-64-6; dipalmitoylphosphatidylethanolamine, 3026-45-7; trehalose, 99-20-7; 1,2-dipalmitoyl-3-phosphatidylethanolamine, 3026-45-7; 1,2-Dipalmitoylphosphatidylcholine, 2644-64-6; Cholesterol, 57-88-5; Phosphatidylethanolamines; Trehalose, 99-20-7
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00052736_v1713_n1_p57_Ohtake.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052736_v1713_n1_p57_Ohtake

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Citas:

---------- APA ----------
Ohtake, S., Schebor, C., Palecek, S.P. & De Pablo, J.J. (2005) . Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose. Biochimica et Biophysica Acta - Biomembranes, 1713(1), 57-64.
http://dx.doi.org/10.1016/j.bbamem.2005.05.001
---------- CHICAGO ----------
Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J. "Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose" . Biochimica et Biophysica Acta - Biomembranes 1713, no. 1 (2005) : 57-64.
http://dx.doi.org/10.1016/j.bbamem.2005.05.001
---------- MLA ----------
Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J. "Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose" . Biochimica et Biophysica Acta - Biomembranes, vol. 1713, no. 1, 2005, pp. 57-64.
http://dx.doi.org/10.1016/j.bbamem.2005.05.001
---------- VANCOUVER ----------
Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J. Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose. Biochim. Biophys. Acta Biomembr. 2005;1713(1):57-64.
http://dx.doi.org/10.1016/j.bbamem.2005.05.001