Navegar

Colección

Datos Estadísticas

Artículo

Vittori, D.; Garbossa, G.; Lafourcade, C.; Pérez, G.; Nesse, A. "Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein" (2002) Biochimica et Biophysica Acta - Biomembranes. 1558(2):142-150
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

There is evidence that anaemia is associated with aluminium (Al). We have already reported on the sensitivity to Al, showed by erythroid cell populations of animals chronically exposed to the metal. In order to investigate whether Al could also affect human cells, experiments were carried out both on immature and mature human erythroid cells. Erythroid progenitors (CFU-E, colony-forming units-erythroid) concentrated from human peripheral blood were cultured in an Al-rich medium under erythropoietin stimulation and their development analysed. Human peripheral erythrocytes were aged in the presence of Al. Cells were examined using scanning electron microscopy, and membrane proteins analysed by polyacrylamide gel electrophoresis with sodium dodecyl sulphate and immunoblotting. The development of the Al-treated progenitors was 8750/6600-9200 CFU-E/10 6 cells, a significantly lower median value (P<0.05) than that showed by non-treated cells (12 300/11 200-20 700 CFU-E/10 6 cells). Erythrocyte morphological changes were induced by Al during the in vitro ageing. The cells lost their typical biconcave shape, turning into acanthocytes and stomatocytes. Simultaneously, an increased membrane protein breakdown compatible with band 3 degradation was detected. Besides, Al was found within the cells and attached to the membrane. The present in vitro results suggest that Al may disturb human erythropoiesis through combined effects on mature erythrocytes and cellular metabolism in late erythroid progenitors. © 2002 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein
Autor:Vittori, D.; Garbossa, G.; Lafourcade, C.; Pérez, G.; Nesse, A.
Filiación:Laboratorio De Análisis Biológicos, Departamento De Química Biológica, Universidad De Buenos Aires, Pabellón II, 1428 Buenos Aires, Argentina
Palabras clave:Aluminium toxicity; Anaemia; Band 3; Erythrocyte morphology; Membrane proteins; aluminum; dodecyl sulfate sodium; erythrocyte band 3 protein; erythropoietin; anemia; article; cell culture; cell maturation; cell metabolism; cell stimulation; colony forming unit; controlled study; culture medium; erythrocyte structure; erythroid cell; erythropoiesis; human; human cell; immunoblotting; polyacrylamide gel electrophoresis; priority journal; protein analysis; protein degradation; scanning electron microscopy; stem cell; Aluminum Compounds; Anion Exchange Protein 1, Erythrocyte; Cytosol; Erythrocyte Aging; Erythrocyte Membrane; Erythrocytes; Erythroid Progenitor Cells; Humans; Immunoblotting; Time Factors; Animalia
Año:2002
Volumen:1558
Número:2
Página de inicio:142
Página de fin:150
DOI: http://dx.doi.org/10.1016/S0005-2736(01)00427-8
Título revista:Biochimica et Biophysica Acta - Biomembranes
Título revista abreviado:Biochim. Biophys. Acta Biomembr.
ISSN:00052736
CODEN:BBBMB
CAS:Aluminum Compounds; Anion Exchange Protein 1, Erythrocyte
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052736_v1558_n2_p142_Vittori

Referencias:

  • Elliot, H.L., McDougall, A.I., Aluminum studies in dialysis encephalopathy (1978) Proc. Eur. Dial. Transpl. Assoc., 15, pp. 157-163
  • Wills, M.R., Savory, J., Aluminium poisoning dialysis encephalopathy, osteomalacia, and anaemia (1983) Lancet, 2, pp. 29-34
  • Eschbach, J.W., Adamson, J.W., Anemia of end-stage renal disease (ESRD) (1985) Kidney Int., 28, pp. 1-5
  • Abreo, K., Brown, S.T., Sella, M., Trapp, G., Application of an erythrocyte aluminum assay in the diagnosis of aluminum-associated microcytic anemia in patients undergoing dialysis and response to deferoxamine therapy (1989) J. Lab. Clin. Med., 113, pp. 50-57
  • Seibert, F.B., Wells, H.G., The effect of aluminum on mammalian blood and tissues (1929) Arch. Pathol., 8, pp. 230-261
  • Kaiser, L., Schwartz, K.A., Burnatowska-Hledin, M.A., Mayor, G.H., Microcytic anemia secondary to intraperitoneal aluminum in normal and uremic rats (1984) Kidney Int., 26, pp. 269-274
  • Drüeke, T.B., Lacour, B., Touam, M., Jucquel, J.P., Plachot, J.J., Cournot-Witmer, G., Galle, P., Effect of aluminum on hematopoiesis (1986) Kidney Int., 29 (SUPPL. 18), pp. S45-S48
  • Garbossa, G., Gutnisky, A., Nesse, A., Depressed erythroid progenitor cell activity in aluminum-overloaded mice (1996) Miner. Electrolyte Metab., 22, pp. 214-218
  • Garbossa, G., Gálvez, G., Castro, M.E., Nesse, A., Oral aluminum administration to rats with normal renal function. 1. Impairment of erythropoiesis (1998) Hum. Exp. Toxicol., 17, pp. 312-317
  • Vittori, D., Nesse, A., Pérez, G., Garbossa, G., Morphological and functional alterations of erythroid cells induced by long-term ingestion of aluminium (1999) J. Inorg. Biochem., 76, pp. 113-120
  • Zaman, K., Dabrowski, Z., Miszta, H., Effects of aluminium on erythroidal cells in bone marrow in rats (1990) Folia Histochem. Cytobiol., 28, pp. 61-68
  • Suwalsky, M., Ungerer, B., Villena, F., Norris, B., Cardenas, H., Zatta, P., Interactions of Al(acac) 3 with cell membranes and model phospholipid bilayer (1999) J. Inorg. Biochem., 75, pp. 263-268
  • Mohandas, N., Morrow, J.S., Plasma membrane dynamics and organization (2000) Hematology. Basic Principles and Practice, pp. 40-48. , R. Hoffman, E.J. Benz, S.J. Shattil, B. Furie, H.J. Cohen, L.E. Silverstein, P. McGlave (Eds.), Churchill Livingstone, New York
  • Garbossa, G., Gutnisky, A., Nesse, A., The inhibitory action of aluminum on mouse bone marrow cell growth: Evidence for an erythropoietin- and transferrin-mediated mechanism (1994) Miner. Electrolyte Metab., 20, pp. 141-146
  • Worthington, R.E., Bossie-Condreanu, J., Van Zant, G., Quantification of erythroid differentiation in vitro using a sensitive colorimetric assay for hemoglobin (1987) Exp. Hematol., 15, pp. 85-92
  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
  • Lowry, O.H., Roseborough, N.J., Farr, A.L., Randall, R.L., Protein measurements with Folin reagent (1951) J. Biol. Chem., 193, pp. 267-275
  • Dzandu, J.K., Deh, M.E., Barratt, D.L., Wise, G.E., Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique (1984) Cell Biol., 81, pp. 1733-1737
  • Towbin, H., Staehelin, T., Gordon, J., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications (1979) Proc. Natl. Acad. Sci. USA, 76, pp. 4350-4354
  • Zatta, P., Perazzolo, M., Corain, B., Tris acetylacetonate aluminium (III) induces osmotic fragility and acanthocyte formation in suspended erythrocytes (1989) Toxicol. Lett., 45, pp. 15-21
  • Tanner, M.J.A., Molecular and cellular biology of the erythrocyte anion exchanger (AE1) (1993) Semin. Hematol., 30, pp. 34-57
  • Kay, M.M.B., Flowers, N., Goodman, J., Bosman, G., Alterations in membrane protein band 3 associated with accelerated erythrocyte aging (1989) Proc. Natl. Acad. Sci. USA, 86, pp. 5834-5838
  • Schwarsz-BenMeir, N., Glaser, T., Kosower, N.S., Band 3 protein degradation by calpain is enhanced in erythrocyte of old people (1991) Biochem. J., 275, pp. 47-52
  • Guo-Ross, S., Yang, E., Bondy, S.C., Elevation of cerebral proteases after systemic administration of aluminium (1998) Neurochem. Int., 33, pp. 277-282
  • Tanner, M.J.A., The structure and function of band 3 (AE1): Recent developments (1997) Mol. Memb. Biol., 14, pp. 155-165
  • Sheetz, M.P., Singer, S.J., Biological membranes as bilayer couples. A molecular mechanism of drug-erythrocyte interactions (1974) Proc. Natl. Acad. Sci. USA, 71, pp. 4457-4461
  • Van Rensburg, S.J., Carstens, M.E., Potocnik, F.C.V., Van der Spuy, G., Van der Walt, B.J., Taljaard, J.J.F., Transferrin C2 and Alzheimer's disease; another piece of the puzzle found? (1995) Med. Hypothesis, 44, pp. 268-272

Citas:

---------- APA ----------
Vittori, D., Garbossa, G., Lafourcade, C., Pérez, G. & Nesse, A. (2002) . Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein. Biochimica et Biophysica Acta - Biomembranes, 1558(2), 142-150.
http://dx.doi.org/10.1016/S0005-2736(01)00427-8
---------- CHICAGO ----------
Vittori, D., Garbossa, G., Lafourcade, C., Pérez, G., Nesse, A. "Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein" . Biochimica et Biophysica Acta - Biomembranes 1558, no. 2 (2002) : 142-150.
http://dx.doi.org/10.1016/S0005-2736(01)00427-8
---------- MLA ----------
Vittori, D., Garbossa, G., Lafourcade, C., Pérez, G., Nesse, A. "Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein" . Biochimica et Biophysica Acta - Biomembranes, vol. 1558, no. 2, 2002, pp. 142-150.
http://dx.doi.org/10.1016/S0005-2736(01)00427-8
---------- VANCOUVER ----------
Vittori, D., Garbossa, G., Lafourcade, C., Pérez, G., Nesse, A. Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein. Biochim. Biophys. Acta Biomembr. 2002;1558(2):142-150.
http://dx.doi.org/10.1016/S0005-2736(01)00427-8