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Tosteson, M.T.; Kim, J.B.; Goldstein, D.J.; Tosteson, D.C. "Ion channels formed by transcription factors recognize consensus DNA sequences" (2001) Biochimica et Biophysica Acta - Biomembranes. 1510(1-2):209-218
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Abstract:

Transcription factors (TFs) are proteins which bind to specific DNA sequences and thus participate in the regulation of the initiation of transcription. We report in this communication our observations that several of these proteins interact with lipid membranes and form ion-permeable channels. For each of the TFs that we studied, the single channel conductance was distinctively different, i.e. each TF had its own electrical signature. More importantly, we show for the first time that addition of cognate double-stranded DNA sequences leads to a specific response: an increase in the conductance of the TF-containing membrane. Strikingly, the effect of cognate DNA was observed when it was added to the trans-side of the membrane (opposite to where the TF was added), strongly suggesting that the TFs span the membrane and that the DNA-binding domain is trans-accessible. Alterations in the primary structure of the TF factors in their basic and DNA-binding regions change the characteristics of the conductance of the protein-containing membranes as well as the response to DNA addition, reinforcing the notion that the changes we measure are due to specific interactions. © 2001 Elsevier Science B.V.

Registro:

Documento: Artículo
Título:Ion channels formed by transcription factors recognize consensus DNA sequences
Autor:Tosteson, M.T.; Kim, J.B.; Goldstein, D.J.; Tosteson, D.C.
Filiación:Laboratory for Membrane Transport, Department of Cell Biology, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115, United States
School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea
Facultad de Ciencias Exactas y Naturals, National University of Buenos Aires, Buenos Aires, Argentina
Palabras clave:DNA binding; Lipid bilayer; Protein channel; Transcription factor; double stranded DNA; ion channel; transcription factor; article; consensus sequence; DNA binding; DNA sequence; membrane binding; membrane conductance; priority journal; protein lipid interaction; Consensus Sequence; Dimerization; DNA; Ion Channels; Lipid Bilayers; Membrane Potentials; Molecular Conformation; Protein Binding; Transcription Factors
Año:2001
Volumen:1510
Número:1-2
Página de inicio:209
Página de fin:218
DOI: http://dx.doi.org/10.1016/S0005-2736(00)00351-5
Título revista:Biochimica et Biophysica Acta - Biomembranes
Título revista abreviado:Biochim. Biophys. Acta Biomembr.
ISSN:00052736
CODEN:BBBMB
CAS:DNA, 9007-49-2; Ion Channels; Lipid Bilayers; Transcription Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052736_v1510_n1-2_p209_Tosteson

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Citas:

---------- APA ----------
Tosteson, M.T., Kim, J.B., Goldstein, D.J. & Tosteson, D.C. (2001) . Ion channels formed by transcription factors recognize consensus DNA sequences. Biochimica et Biophysica Acta - Biomembranes, 1510(1-2), 209-218.
http://dx.doi.org/10.1016/S0005-2736(00)00351-5
---------- CHICAGO ----------
Tosteson, M.T., Kim, J.B., Goldstein, D.J., Tosteson, D.C. "Ion channels formed by transcription factors recognize consensus DNA sequences" . Biochimica et Biophysica Acta - Biomembranes 1510, no. 1-2 (2001) : 209-218.
http://dx.doi.org/10.1016/S0005-2736(00)00351-5
---------- MLA ----------
Tosteson, M.T., Kim, J.B., Goldstein, D.J., Tosteson, D.C. "Ion channels formed by transcription factors recognize consensus DNA sequences" . Biochimica et Biophysica Acta - Biomembranes, vol. 1510, no. 1-2, 2001, pp. 209-218.
http://dx.doi.org/10.1016/S0005-2736(00)00351-5
---------- VANCOUVER ----------
Tosteson, M.T., Kim, J.B., Goldstein, D.J., Tosteson, D.C. Ion channels formed by transcription factors recognize consensus DNA sequences. Biochim. Biophys. Acta Biomembr. 2001;1510(1-2):209-218.
http://dx.doi.org/10.1016/S0005-2736(00)00351-5