Abstract:
Native chloroplast fructose-1,6-bisphosphatase (d-fructose 1,6-bisphosphate-l-phosphohydrolase, EC 3.1.3.11) wasinactivated with either Woodward's reagent K or diethylpyrocarbonate. Since pseudo-first-order rate constants (κapp) were a hyperbolic function of inhibitor concentration, it appeared that native chloroplast fructose-1,6-bisphosphatase and either Woodward's reagent K or diethylpyrocarbonate interacted reversibly prior to formation of irreversible (inactive) complex. Protection against inactivation was afforded by preincubating the enzyme with dithiothreitol, fructose 1,6-bisphosphate, Ca2+ and either chloroplast thioredoxin-f or a chaotropic anion (trichloroacetate). Following similar incubation with an organic solvent (2-propanol), the catalytic activity remained unaltered in the presence of Woodward's reagent K but was inactivated by diethylpyrocarbonate. The enhancement of the specific activity of chloroplast fructose-1,6-bisphosphatase caused by dithiothreitol, fructose-1,6-bisphosphate, Ca2+ and either chloroplast thioredoxin-f, or chaotropic anions or cosolvents was similar. Therefore, differential reactivity to selected reagents indicated the existence of several conformations of chloroplast fructose-1,6-bisphosphatase, i.e., native and different active forms. © 1989 Elsevier Science Publishers B.V. (Biomedical Division).
Registro:
Documento: |
Artículo
|
Título: | Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate |
Autor: | Prat-Gay, G.; Wolosiuk, RicardoA. |
Filiación: | Institulo de Iiwestigaciones Bioqu'imicas, Facullad de Ciencias Exailasy Naturales (U.B.A.). Buenos Aires, Argentina
|
Palabras clave: | Chaotropic anion; Diethylpyrocarbonate: Thioredoxin; Fructose-l,6-bisphosphalase: Woodward's reagent K; Organic solvent |
Año: | 1989
|
Volumen: | 973
|
Número: | 3
|
Página de inicio: | 457
|
Página de fin: | 464
|
DOI: |
http://dx.doi.org/10.1016/S0005-2728(89)80388-3 |
Título revista: | BBA - Bioenergetics
|
Título revista abreviado: | Biochim. Biophys. Acta Bioenerg.
|
ISSN: | 00052728
|
CODEN: | BBBEB
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052728_v973_n3_p457_PratGay |
Referencias:
- Dilley, Vemon, (1965) Arch. Biochem. Biophys., 111, pp. 365-375
- Lilley, Chon, Mosbach, Heldt, (1977) Biochim. Biophys. Acta, 460, pp. 259-272
- Anderson, (1979) Encycl. Plant Physiol., 6, pp. 271-281
- Huber, (1982) Cellular and Subcellular Localization in Plant Metabolism, pp. 151-184. , Creasy L.L., Hrazdina G., Plenum Press, Tokyo
- Buchanan, Wolosiuk, Schurmann, (1979) Trends Biochem. Sci., 4, pp. 93-96
- Frieden, (1979) Annu. Rev. Biochem., 48, pp. 471-489
- Wolosiuk, Hertig, (1983) Thioredoxins, Structure and Function, pp. 167-173. , Paris, Gadal P., Editions du CNRS
- Hertig, Wolosiuk, (1980) Biochem. Biophys. Res. Commun., 97, pp. 325-333
- Hertig, Wolosiuk, (1983) J.Biol. Chem., 258, pp. 984-989
- Corley, Wolosiuk, (1985) J. Biol. Chem., 260, pp. 3978-3983
- Stein, Wolosiuk, (1987) J Biol Chem, 262, pp. 16171-16179
- Buchanan, Schumann, Kalberer, (1971) J.Biol. Chem., 246, pp. 5952-5959
- Pradel, Soulie, Buc, Meunier, Ricard, (1981) Eur. J. Biochem., 113, pp. 507-511
- Aragnol, Pradel, Cecchini, (1985) Biochim. Biophys. Acta, 829, pp. 275-281
- Bodlaender, Feinstein, Shaw, (1969) Biochemistry, 8, pp. 4941-4949
- Muhlrad, Hegyi, Horanyi, (1969) Biochimica et Biophysica Acta (BBA) - Protein Structure, 181, pp. 184-190
- Miles, (1977) Methods Enzymol., 47, pp. 431-442
- Wolosiuk, Schurmann, Buchanan, (1980) Methods Enzymol., 69-100, pp. 382-391
- Laemmli, (1970) Nature, 227, pp. 680-685
- Schurmann, Wolosiuk, (1978) Biochim. Biophys. Acta, 522, pp. 130-138
- Chen, Toribara, Warner, (1956) Anal. Chem., 28, pp. 1756-1758
- Levy, Leber, Ryan, (1963) J.Biol. Chem., 238, pp. 3654-3659
- Carlson, (1984) Biochim. Biophys. Acta, 789, pp. 347-350
- Meloche, (1967) Biochemistry, 6, pp. 2273-2280
- Petra, (1971) Biochemistry, 10, pp. 3163-3170
- Feinstein, Bodlaender, Shaw, (1969) Biochemistry, 8, pp. 4949-4955
- Dunn, Anfinsen, Shrager, (1974) J.Biol. Chem., 249, pp. 3717-3723
- Pradel, Aragnol, (1986) Biochim. Biophys. Acta, 871, pp. 293-301
- Holmgren, (1985) Annu. Rev. Biochem., 54, pp. 237-272
- Marcus, Harrsch, Moberly, Edelstein, Latshaw, (1987) Biochemistry, 26, pp. 7029-7035
- Wolosiuk, Corley, Crawford, Buchanan, (1985) FEBS Lett., 189, pp. 212-217
- Crawford, Yee, Hutchenson, Wolosiuk, Buchanan, (1986) Arch. Biochem. Biophys., 244, pp. 1-15
- Wolosiuk, Hertig, Busconi, (1986) Arch. Biochem. Biophys., 246, pp. 1-8
- Wolosiuk, Stein, Busconi, (1987) Progress in Photosynthesis Research, 3, pp. 459-462. , Biggins J., Martinus Nijhoff, New York
Citas:
---------- APA ----------
Prat-Gay, G. & Wolosiuk, RicardoA.
(1989)
. Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate. BBA - Bioenergetics, 973(3), 457-464.
http://dx.doi.org/10.1016/S0005-2728(89)80388-3---------- CHICAGO ----------
Prat-Gay, G., Wolosiuk, RicardoA.
"Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate"
. BBA - Bioenergetics 973, no. 3
(1989) : 457-464.
http://dx.doi.org/10.1016/S0005-2728(89)80388-3---------- MLA ----------
Prat-Gay, G., Wolosiuk, RicardoA.
"Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate"
. BBA - Bioenergetics, vol. 973, no. 3, 1989, pp. 457-464.
http://dx.doi.org/10.1016/S0005-2728(89)80388-3---------- VANCOUVER ----------
Prat-Gay, G., Wolosiuk, RicardoA. Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate. Biochim. Biophys. Acta Bioenerg. 1989;973(3):457-464.
http://dx.doi.org/10.1016/S0005-2728(89)80388-3