Artículo
Abstract:
1. By means of different fractionation procedures we have separated two major protein fractions present in photosynthetically grown cells of the blue-green mutant of Rhodospirillum rubrum (BG1). These proteins are found in differing amounts in the two subchromatophore fragments produced by the detergent Triton X-100. A more extensive breakdown caused by digestion of intact photosynthetic membrane with Triton X-100 and trypsin produced four main protein fractions which were separated by means of gel electrophoresis. Some of these bands originated from proteins that were intimately associated with bacteriochlorophyll in the intact membrane. The other protein fractions seem to derive from a group of proteins whose function could be a supporting one. These protein fractions seem to be a somewhat degraded form of the basic membrane core that cannot be further attacked by the combined action of the detergent and the trypsin. 2. Electron micrographs of the fractions obtained by the digestion with Triton X-100 and trypsin are presented, showing a very different structure for the degraded basic membrane and a unique structure for the aggregated units of the bacteriochlorophyll-containing fraction. © 1972.
Registro:
| Documento: | Artículo |
| Título: | Protein composition of intact and fractionated memberanes isolated from dark and light grown cells of a blue green mutant of Rhodospirillum Rubrum (BG1) |
| Autor: | Kerber, N.L.; García, A.F.; Vernon, L.P.; Raveed, D. |
| Filiación: | Centro de Investigaciones Microbiológicas, Facultad de Ciencias Exactas y Naturales, UNBA, Buenos Aires, Argentina Charles F. Kettering Research Laboratory, Yellow Springs, OH, United States |
| Palabras clave: | bacterial protein; chlorophyll; Surface Active Agents; surfactant; trypsin; urea; adaptation; article; bacterial chromatophore; cell fractionation; cytology; darkness; electron microscopy; gel electrophoresis; light; membrane; metabolism; mutation; osmosis; photosynthesis; radiation exposure; radiation response; Rhodospirillum; Rhodospirillum rubrum; Adaptation, Physiological; Bacterial Chromatophores; Bacterial Proteins; Cell Fractionation; Chlorophyll; Darkness; Electrophoresis, Disc; Light; Membranes; Microscopy, Electron; Mutation; Osmosis; Photosynthesis; Radiation Effects; Rhodospirillum; Rhodospirillum rubrum; Subcellular Fractions; Surface-Active Agents; Trypsin; Urea |
| Año: | 1972 |
| Volumen: | 256 |
| Número: | 1 |
| Página de inicio: | 108 |
| Página de fin: | 119 |
| DOI: | http://dx.doi.org/10.1016/0005-2728(72)90166-1 |
| Título revista: | BBA - Bioenergetics |
| Título revista abreviado: | Biochim. Biophys. Acta Bioenerg. |
| ISSN: | 00052728 |
| CODEN: | BBBEB |
| CAS: | chlorophyll, 1406-65-1, 15611-43-5; trypsin, 9002-07-7; urea, 57-13-6; Bacterial Proteins; Chlorophyll, 1406-65-1; Surface-Active Agents; Trypsin, EC 3.4.21.4; Urea, 57-13-6 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052728_v256_n1_p108_Kerber |
Referencias:
- Vernon, Garcfa, (1967) Biochim. Biophys. Acta, 143, p. 144
- Garcfa, Vernon, Mollenhauer, (1966) Biochemistry, 5, p. 2399
- Garcfa, Vernon, Mollenhauer, (1966) Biochemistry, 5, p. 2408
- Cellarius, Peters, (1969) Biochim. Biophys. Acta, 189, p. 234
- Newton, (1961) Methods in Enzymology, 1, p. 70. , S.P. Colowick, N.O. Kaplan, Academic Press, New York
- Robrish, Marr, (1962) J. Bacteriol., 83, p. 160
- Schlessinger, (1963) J. Mol. Biol., 7, p. 569
- Takayama, McLennan, Tzagoloff, Stoner, (1966) Arch. Biochem. Biophys., 114, p. 223
- Lowry, Rosebrough, Farr, Randall, (1951) J. Biol. Chem., 193, p. 265
- Clayton, (1963) Bacterial Photosynthesis, p. 495. , H. Gest, A. San Pietro, L.P. Vernon, Antioch, Yellow Springs, Ohio
- Callahan, Horner, (1964) J. Cell Biol., 20, p. 350
- Rivas, Kerber, Viale, Garcia, (1970) FEBS Lett., 11, p. 37
- Gorchein, (1964) Biochim. Biophys. Acta, 84, p. 356
Citas:
---------- APA ----------
Kerber, N.L., García, A.F., Vernon, L.P. & Raveed, D. (1972) . Protein composition of intact and fractionated memberanes isolated from dark and light grown cells of a blue green mutant of Rhodospirillum Rubrum (BG1). BBA - Bioenergetics, 256(1), 108-119.http://dx.doi.org/10.1016/0005-2728(72)90166-1
---------- CHICAGO ----------
Kerber, N.L., García, A.F., Vernon, L.P., Raveed, D. "Protein composition of intact and fractionated memberanes isolated from dark and light grown cells of a blue green mutant of Rhodospirillum Rubrum (BG1)" . BBA - Bioenergetics 256, no. 1 (1972) : 108-119.http://dx.doi.org/10.1016/0005-2728(72)90166-1
---------- MLA ----------
Kerber, N.L., García, A.F., Vernon, L.P., Raveed, D. "Protein composition of intact and fractionated memberanes isolated from dark and light grown cells of a blue green mutant of Rhodospirillum Rubrum (BG1)" . BBA - Bioenergetics, vol. 256, no. 1, 1972, pp. 108-119.http://dx.doi.org/10.1016/0005-2728(72)90166-1
---------- VANCOUVER ----------
Kerber, N.L., García, A.F., Vernon, L.P., Raveed, D. Protein composition of intact and fractionated memberanes isolated from dark and light grown cells of a blue green mutant of Rhodospirillum Rubrum (BG1). Biochim. Biophys. Acta Bioenerg. 1972;256(1):108-119.http://dx.doi.org/10.1016/0005-2728(72)90166-1
