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Abstract:

The cAMP binding domain of the regulatory subunit (R) of Mucor rouxii protein kinase A was cloned. The deduced amino acid sequence was highly homologous in sequence and in size to the corresponding region in fungal and higher eukaryotic regulatory subunits (47-54%), but particularly homologous (62%) to Blastocladiella emersonii, a fungus classified in a different phylum. Amino acids reported to be important for interaction with cAMP, for cooperativity between the two cAMP binding domains, in the general folding of the domain, and for interaction with the catalytic subunit were conserved in all the fungal sequences. Based on either sequence or functional behavior, the M. rouxii R subunit cannot be classified as being more similar to RI or RII of mammalian systems. The M. rouxii protein sequence was modeled using as template the coordinates of the crystallized bovine regulatory subunit type I(α). The quality of the model is good. The two backbones could be perfectly overlapped, except for two loop regions of high divergence. The α helix C of domain A, proposed to have a strong interaction with the catalytic subunit, contains a leucine replacing a basic residue (arginine or lysine) commonly found in RI or RII. The domains A and B of the M. rouxii regulatory subunit were overexpressed as fusion proteins with GST. GST domain B protein was inactive. GST domain A was active; the kinetic parameters of affinity toward cAMP analogs, site selectivity, and dissociation kinetics of bound cAMP were analogous to the properties of the domain in the whole regulatory subunit. (C) 2000 Academic Press.

Registro:

Documento: Artículo
Título:Structural and functional analysis of the cAMP binding domain from the regulatory subunit of Mucor rouxii protein kinase A
Autor:Sorol, M.R.; Pastori, R.L.; Muro, A.; Moreno, S.; Rossi, S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Diabetes Research Institute, University of Miami, Miami, FL 33021, United States
International Center for Genetic Engineering and Biotechnology, Trieste, I-34012, Italy
Palabras clave:cAMP binding; Mucor rouxii; Protein sequence; amino acid; arginine; cyclic AMP; cyclic AMP dependent protein kinase; cyclic AMP derivative; hybrid protein; leucine; lysine; amino acid sequence; article; binding affinity; chemical structure; dissociation; enzyme active site; molecular model; Mucor; nonhuman; nucleotide sequence; polymerase chain reaction; priority journal; sequence homology; Amylomyces rouxii; Blastocladiella emersonii; Blastocladiella emersonii; Bovinae; Eukaryota; Fungi; Mammalia; Mucor; Mucor rouxii
Año:2000
Volumen:382
Número:2
Página de inicio:173
Página de fin:181
DOI: http://dx.doi.org/10.1006/abbi.2000.2018
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:amino acid, 65072-01-7; arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; cyclic AMP, 60-92-4; cyclic AMP dependent protein kinase; leucine, 61-90-5, 7005-03-0; lysine, 56-87-1, 6899-06-5, 70-54-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v382_n2_p173_Sorol

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Citas:

---------- APA ----------
Sorol, M.R., Pastori, R.L., Muro, A., Moreno, S. & Rossi, S. (2000) . Structural and functional analysis of the cAMP binding domain from the regulatory subunit of Mucor rouxii protein kinase A. Archives of Biochemistry and Biophysics, 382(2), 173-181.
http://dx.doi.org/10.1006/abbi.2000.2018
---------- CHICAGO ----------
Sorol, M.R., Pastori, R.L., Muro, A., Moreno, S., Rossi, S. "Structural and functional analysis of the cAMP binding domain from the regulatory subunit of Mucor rouxii protein kinase A" . Archives of Biochemistry and Biophysics 382, no. 2 (2000) : 173-181.
http://dx.doi.org/10.1006/abbi.2000.2018
---------- MLA ----------
Sorol, M.R., Pastori, R.L., Muro, A., Moreno, S., Rossi, S. "Structural and functional analysis of the cAMP binding domain from the regulatory subunit of Mucor rouxii protein kinase A" . Archives of Biochemistry and Biophysics, vol. 382, no. 2, 2000, pp. 173-181.
http://dx.doi.org/10.1006/abbi.2000.2018
---------- VANCOUVER ----------
Sorol, M.R., Pastori, R.L., Muro, A., Moreno, S., Rossi, S. Structural and functional analysis of the cAMP binding domain from the regulatory subunit of Mucor rouxii protein kinase A. Arch. Biochem. Biophys. 2000;382(2):173-181.
http://dx.doi.org/10.1006/abbi.2000.2018