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Abstract:

A cAMP-dependent protein kinase from mycelia of Saccobolus platensis was characterized. The holoenzyme seems to be a dimer (i.e., regulatory subunit-catalytic subunit) of 78,000 Da, slightly activated by cAMP but susceptible to dissociation into its subunits by cAMP, or by kemptide and protamine, the best substrates for Saccobolus protein kinase. The regulatory subunit was purified to homogeneity by affinity chromatography. It is highly specific for cAMP and has two types of binding sites but failed to inhibit the phosphotransferase activity of the homologous or the heterologous (bovine heart) catalytic components. The activity of the catalytic subunit was completely abolished by the regulatory component of the bovine heart protein kinase as well as by a synthetic peptide corresponding to the active site of the mammalian protein kinase inhibitor. The data suggest that interaction between the subunits of the S. platensis protein kinase is different than that found in cAMP-dependent protein kinases from other sources. Similarities and differences between the Saccobolus protein kinase and enzymes from low eucaryotes and mammalian tissues are discussed. © 1990.

Registro:

Documento: Artículo
Título:Isolation and characterization of a dimeric cAMP-dependent protein kinase from the fungus Saccobolus platensis
Autor:Silberstein, S.; Cantore, M.L.; Galvagno, M.A.; Passeron, S.
Filiación:Departamentos de Química Biológica y Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires Ciudad Universitaria, 4to. piso, Pabellón II, 1428 Buenos Aires, Argentina
Palabras clave:cyclic amp; protein kinase; radioisotope; article; fungus; nonhuman; priority journal; Ascomycota; Centrifugation, Density Gradient; Chromatography, Affinity; Chromatography, DEAE-Cellulose; Cyclic AMP; Cytosol; Electrophoresis, Polyacrylamide Gel; Kinetics; Macromolecular Systems; Molecular Weight; Protein Conformation; Protein Kinases; Substrate Specificity; Support, Non-U.S. Gov't; Bovinae; Eukaryota; Fungi; Mammalia; Saccobolus
Año:1990
Volumen:282
Número:1
Página de inicio:132
Página de fin:140
DOI: http://dx.doi.org/10.1016/0003-9861(90)90096-H
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:Cyclic AMP, 60-92-4; Macromolecular Systems; Protein Kinases, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v282_n1_p132_Silberstein

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Citas:

---------- APA ----------
Silberstein, S., Cantore, M.L., Galvagno, M.A. & Passeron, S. (1990) . Isolation and characterization of a dimeric cAMP-dependent protein kinase from the fungus Saccobolus platensis. Archives of Biochemistry and Biophysics, 282(1), 132-140.
http://dx.doi.org/10.1016/0003-9861(90)90096-H
---------- CHICAGO ----------
Silberstein, S., Cantore, M.L., Galvagno, M.A., Passeron, S. "Isolation and characterization of a dimeric cAMP-dependent protein kinase from the fungus Saccobolus platensis" . Archives of Biochemistry and Biophysics 282, no. 1 (1990) : 132-140.
http://dx.doi.org/10.1016/0003-9861(90)90096-H
---------- MLA ----------
Silberstein, S., Cantore, M.L., Galvagno, M.A., Passeron, S. "Isolation and characterization of a dimeric cAMP-dependent protein kinase from the fungus Saccobolus platensis" . Archives of Biochemistry and Biophysics, vol. 282, no. 1, 1990, pp. 132-140.
http://dx.doi.org/10.1016/0003-9861(90)90096-H
---------- VANCOUVER ----------
Silberstein, S., Cantore, M.L., Galvagno, M.A., Passeron, S. Isolation and characterization of a dimeric cAMP-dependent protein kinase from the fungus Saccobolus platensis. Arch. Biochem. Biophys. 1990;282(1):132-140.
http://dx.doi.org/10.1016/0003-9861(90)90096-H