Abstract:
Two heat-stable inhibitors (a and b) of phosphoprotein phosphatases I and II from Mucor rouxii were isolated from mycelium of the fungus. They were partially purified from extracts by heating, DEAE-cellulose chromatography, and Sephadex G-75 gel filtration. The molecular weights of inhibitors a and b, estimated by gel filtration, are 5,000 and 20,000 respectively. Inhibitor a acts similarly on both enzymes while inhibitor b is relatively more active on enzyme II. Storage of inhibitor b at -20 °C for several weeks resulted in a partial conversion to a lower-molecular-weight form with properties similar to those of inhibitor a. © 1985.
Registro:
Documento: |
Artículo
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Título: | The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii |
Autor: | Seigelchifer, M.A.; Passeron, S. |
Filiación: | Programa de Regulación Hormonal y Metabólica, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Pabellón 2, 1428 Buenos Aires, Argentina
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Palabras clave: | enzyme inhibitor; phosphatase; phosphoprotein; fungus; mucor rouxii; nonhuman; priority journal; protein phosphorylation; Chemistry; Chromatography, Gel; Heat; Molecular Weight; Mucor; Phosphoprotein Phosphatase; Support, Non-U.S. Gov't |
Año: | 1985
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Volumen: | 238
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Número: | 1
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Página de inicio: | 353
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Página de fin: | 357
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DOI: |
http://dx.doi.org/10.1016/0003-9861(85)90174-2 |
Título revista: | Archives of Biochemistry and Biophysics
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Título revista abreviado: | Arch. Biochem. Biophys.
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ISSN: | 00039861
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CODEN: | ABBIA
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CAS: | phosphatase, 9013-05-2; Phosphoprotein Phosphatase, EC 3.1.3.16
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v238_n1_p353_Seigelchifer |
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Citas:
---------- APA ----------
Seigelchifer, M.A. & Passeron, S.
(1985)
. The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii. Archives of Biochemistry and Biophysics, 238(1), 353-357.
http://dx.doi.org/10.1016/0003-9861(85)90174-2---------- CHICAGO ----------
Seigelchifer, M.A., Passeron, S.
"The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii"
. Archives of Biochemistry and Biophysics 238, no. 1
(1985) : 353-357.
http://dx.doi.org/10.1016/0003-9861(85)90174-2---------- MLA ----------
Seigelchifer, M.A., Passeron, S.
"The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii"
. Archives of Biochemistry and Biophysics, vol. 238, no. 1, 1985, pp. 353-357.
http://dx.doi.org/10.1016/0003-9861(85)90174-2---------- VANCOUVER ----------
Seigelchifer, M.A., Passeron, S. The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii. Arch. Biochem. Biophys. 1985;238(1):353-357.
http://dx.doi.org/10.1016/0003-9861(85)90174-2