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Abstract:

Particulate cell fractions of mycelium of Mucor rouxii contain adenylate cyclase activity which can be partially solubilized by 0.5 m KCl. Homogenates prepared in the presence of Lubrol PX or KCl exhibit two- to threefold more activity compared to homogenates prepared in the absence of detergent or salt. The membrane-bound enzyme prepared in the presence of 2% Lubrol PX was studied in detail. The kinetic properties of this adenylate cyclase were as expected for an allosteric enzyme with the ATP · Mn2- complex as substrate and free Mn2+ ions as activator. Unchelated ATP inhibits the reaction by competing with the substrate. The KCl-solubilized enzyme was partially purified by ion-exchange chromatography, ammonium sulfate precipitation, and gel filtration. Molecular parameters of the solubilized enzyme are: Stokes radius, 8.6 nm; S20,w, 13S; Mr 510,000; and frictional ratio, 1.60. © 1982.

Registro:

Documento: Artículo
Título:Kinetic properties, solubilization, and molecular characterization of Mucor rouxii adenylate cyclase
Autor:Cantore, M.L.; Passeron, S.
Filiación:Programa de Regulación Hormonal y Metabólica, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon 2, 1428 Buenos Aires, Argentina
Palabras clave:adenylate cyclase; article; cell fractionation; enzymology; isolation and purification; kinetics; metabolism; Mucor; solubility; Adenylate Cyclase; Kinetics; Mucor; Solubility; Subcellular Fractions; Support, Non-U.S. Gov't
Año:1982
Volumen:219
Número:1
Página de inicio:1
Página de fin:11
DOI: http://dx.doi.org/10.1016/0003-9861(82)90127-8
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:adenylate cyclase, 9012-42-4; Adenylate Cyclase, EC 4.6.1.1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v219_n1_p1_Cantore

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Citas:

---------- APA ----------
Cantore, M.L. & Passeron, S. (1982) . Kinetic properties, solubilization, and molecular characterization of Mucor rouxii adenylate cyclase. Archives of Biochemistry and Biophysics, 219(1), 1-11.
http://dx.doi.org/10.1016/0003-9861(82)90127-8
---------- CHICAGO ----------
Cantore, M.L., Passeron, S. "Kinetic properties, solubilization, and molecular characterization of Mucor rouxii adenylate cyclase" . Archives of Biochemistry and Biophysics 219, no. 1 (1982) : 1-11.
http://dx.doi.org/10.1016/0003-9861(82)90127-8
---------- MLA ----------
Cantore, M.L., Passeron, S. "Kinetic properties, solubilization, and molecular characterization of Mucor rouxii adenylate cyclase" . Archives of Biochemistry and Biophysics, vol. 219, no. 1, 1982, pp. 1-11.
http://dx.doi.org/10.1016/0003-9861(82)90127-8
---------- VANCOUVER ----------
Cantore, M.L., Passeron, S. Kinetic properties, solubilization, and molecular characterization of Mucor rouxii adenylate cyclase. Arch. Biochem. Biophys. 1982;219(1):1-11.
http://dx.doi.org/10.1016/0003-9861(82)90127-8