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Judewicz, N.D.; Glikin, G.C.; Torres, H.N. "Protein kinase activities in Neurospora crassa" (1981) Archives of Biochemistry and Biophysics. 206(1):87-92
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Abstract:

Several protein kinase activities have been found in 105,000g supernatant of Neurospora crassa mycelia grown up to the logarithmic phase. By chromatography on DEAE-cellulose the following enzyme activities have been resolved: (i) a cyclic AMP-dependent protein kinase (peak I kinase) eluting at 0.20 m NaCl, more active with histone than with phosvitin (it was inhibited by both a thermolabile fraction having cyclic AMP-binding activity and a thermostable inhibitor isolated from 105,0005g mycelial supernates), (ii) a cyclic nucleotide-independent protein kinase (peak II kinase) eluting at 0.35 m NaCl, also more active with histone than with phosvitin (this kinase was not inhibited by the fraction having cyclic AMP-binding activity but it was sensitive to the thermostable inhibitor); and finally, (iii) a protein kinase eluting at 0.43 m NaCl (peak II kinase), with similar activity toward histone and phosvitin, insensitive to cyclic nucleotides and to fractions carrying cyclic AMP-binding capacity (this kinase activity also resulted insensitive to the thermostable inhibiting factor). © 1981.

Registro:

Documento: Artículo
Título:Protein kinase activities in Neurospora crassa
Autor:Judewicz, N.D.; Glikin, G.C.; Torres, H.N.
Filiación:Institute de Investigociones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina
Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:cyclic AMP; protamine kinase; protein kinase; article; binding site; enzymology; ion exchange chromatography; metabolism; Neurospora; Neurospora crassa; Binding Sites; Chromatography, DEAE-Cellulose; Cyclic AMP; Neurospora; Neurospora crassa; Protamine Kinase; Protein Kinases
Año:1981
Volumen:206
Número:1
Página de inicio:87
Página de fin:92
DOI: http://dx.doi.org/10.1016/0003-9861(81)90069-2
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:cyclic AMP, 60-92-4; protamine kinase, 9068-21-7; protein kinase, 9026-43-1; Cyclic AMP, 60-92-4; Protamine Kinase, EC 2.7.1.70; Protein Kinases, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v206_n1_p87_Judewicz

Referencias:

  • Tellez-Iñón, Torres, Interconvertible Forms of Glycogen Phosphorylase in Neurospora crassa (1970) Proceedings of the National Academy of Sciences, 66, pp. 459-463. , 3rd ed
  • Flawiá, Tellez-iñón, Torees, (1972) Biochemistry of the Glycosidic Linkage, pp. 541-571. , R. Piras, H.G. Pontis, Academic Press, New York
  • Terenzi, Flawiá, Torres, (1974) Biochem. Biophys. Res. Commun, 58, pp. 990-996
  • Torres, Flawiá, Terenzi, Tellez-Iñón, (1975) Advan. Cyclic Nucleotide Res, 5, pp. 67-78
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Citas:

---------- APA ----------
Judewicz, N.D., Glikin, G.C. & Torres, H.N. (1981) . Protein kinase activities in Neurospora crassa. Archives of Biochemistry and Biophysics, 206(1), 87-92.
http://dx.doi.org/10.1016/0003-9861(81)90069-2
---------- CHICAGO ----------
Judewicz, N.D., Glikin, G.C., Torres, H.N. "Protein kinase activities in Neurospora crassa" . Archives of Biochemistry and Biophysics 206, no. 1 (1981) : 87-92.
http://dx.doi.org/10.1016/0003-9861(81)90069-2
---------- MLA ----------
Judewicz, N.D., Glikin, G.C., Torres, H.N. "Protein kinase activities in Neurospora crassa" . Archives of Biochemistry and Biophysics, vol. 206, no. 1, 1981, pp. 87-92.
http://dx.doi.org/10.1016/0003-9861(81)90069-2
---------- VANCOUVER ----------
Judewicz, N.D., Glikin, G.C., Torres, H.N. Protein kinase activities in Neurospora crassa. Arch. Biochem. Biophys. 1981;206(1):87-92.
http://dx.doi.org/10.1016/0003-9861(81)90069-2