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Tolmasky, M.E.; Staneloni, R.J.; Ugalde, R.A.; Leloir, L.F. "Lipid-bound sugars in Rhizobium meliloti" (1980) Archives of Biochemistry and Biophysics. 203(1):358-364
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Abstract:

Incubation of an enzyme preparation of Rhizobium meliloti with labeled uridine diphosphate glucose led to the formation of radioactive substances soluble in organic solvents. These substances are probably polyprenyl diphosphate saccharides. They behaved like these on treatment with ammonia or with hot phenol and were decomposed by heating for 10 min at pH 2 yielding a mono- and a disaccharide. The monosaccharide was identified as galactose by paper chromatography. The disaccharide gave glucose and galactose by acid hydrolysis. Following reduction with borohydride it yielded glucose and galactitol. After treatment with periodate followed by paper chromatography only galactose was detectable. The disaccharide was hydrolyzed by β- but not by α-glucosidase. Therefore the disaccharide is glucosyl β1-3-galactose. © 1980, All rights reserved.

Registro:

Documento: Artículo
Título:Lipid-bound sugars in Rhizobium meliloti
Autor:Tolmasky, M.E.; Staneloni, R.J.; Ugalde, R.A.; Leloir, L.F.
Filiación:Instituto de Investigaciones Bioquímicas “Fundación Campomar”, Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:disaccharide; galactose; glucosyltransferase; isoprenoid phosphate sugar; solabiose; uridine diphosphate glucose; article; biosynthesis; enzyme specificity; enzymology; kinetics; metabolism; paper chromatography; Rhizobium; temperature; thin layer chromatography; Chromatography, Paper; Chromatography, Thin Layer; Disaccharides; Galactose; Glucosyltransferases; Kinetics; Polyisoprenyl Phosphate Sugars; Rhizobium; Substrate Specificity; Temperature; Uridine Diphosphate Glucose
Año:1980
Volumen:203
Número:1
Página de inicio:358
Página de fin:364
DOI: http://dx.doi.org/10.1016/0003-9861(80)90187-3
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CAS:galactose, 26566-61-0, 50855-33-9, 59-23-4; glucosyltransferase, 9031-48-5; uridine diphosphate glucose, 133-89-1; Disaccharides; Galactose, 26566-61-0, EC 2.4.1.-; Glucosyltransferases, EC 2.4.1.-; Polyisoprenyl Phosphate Sugars; solabiose, 75023-56-2, 133-89-1; Uridine Diphosphate Glucose, 133-89-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v203_n1_p358_Tolmasky

Referencias:

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Citas:

---------- APA ----------
Tolmasky, M.E., Staneloni, R.J., Ugalde, R.A. & Leloir, L.F. (1980) . Lipid-bound sugars in Rhizobium meliloti. Archives of Biochemistry and Biophysics, 203(1), 358-364.
http://dx.doi.org/10.1016/0003-9861(80)90187-3
---------- CHICAGO ----------
Tolmasky, M.E., Staneloni, R.J., Ugalde, R.A., Leloir, L.F. "Lipid-bound sugars in Rhizobium meliloti" . Archives of Biochemistry and Biophysics 203, no. 1 (1980) : 358-364.
http://dx.doi.org/10.1016/0003-9861(80)90187-3
---------- MLA ----------
Tolmasky, M.E., Staneloni, R.J., Ugalde, R.A., Leloir, L.F. "Lipid-bound sugars in Rhizobium meliloti" . Archives of Biochemistry and Biophysics, vol. 203, no. 1, 1980, pp. 358-364.
http://dx.doi.org/10.1016/0003-9861(80)90187-3
---------- VANCOUVER ----------
Tolmasky, M.E., Staneloni, R.J., Ugalde, R.A., Leloir, L.F. Lipid-bound sugars in Rhizobium meliloti. Arch. Biochem. Biophys. 1980;203(1):358-364.
http://dx.doi.org/10.1016/0003-9861(80)90187-3