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Abstract:

Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele ("crisp") exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not for the "crisp" morphology and recessive in heterocaryons carrying mutated and wild-type cr-1 alleles. The reduced adenylate cyclase activity detected in extracts from cr-1 mutants was not due to an impairment in the extraction of membranes, to a preferential inactivation of the enzyme after extraction, or to the presence of inhibitors. © 1977.

Registro:

Documento: Artículo
Título:Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity
Autor:Flawiá, M.M.; Terenzi, H.F.; Torres, H.N.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar and Facultad de Ciencias Exactas y Naturales, Buenos Aires (28), Argentina
Department of Molecular Biology, The Wellcome Research Laboratories, Burroughs Wellcome Company, 3030 Cornwallis Road, Research Triangle Park, NC 27709, United States
Palabras clave:adenylate cyclase; enzyme deficiency; in vitro study; microorganism; mutant; neurospora crassa; theoretical study; Adenylate Cyclase; Crosses, Genetic; Membranes; Mutation; Neurospora; Neurospora crassa
Año:1977
Volumen:180
Número:2
Página de inicio:334
Página de fin:342
DOI: http://dx.doi.org/10.1016/0003-9861(77)90046-7
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:adenylate cyclase, 9012-42-4; Adenylate Cyclase, EC 4.6.1.1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v180_n2_p334_Flawia

Referencias:

  • Flawiá, Tellez-Iñón, Torres, (1972) Biochemistry of the Glycosidic Linkage, pp. 541-571. , R. Piras, H.G. Pontis, Academic Press, New York
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  • Terenzi, Flawiá, Tellez-Iñón, Torres, (1976) J. Bacteriol, 126, pp. 91-99
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Citas:

---------- APA ----------
Flawiá, M.M., Terenzi, H.F. & Torres, H.N. (1977) . Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity. Archives of Biochemistry and Biophysics, 180(2), 334-342.
http://dx.doi.org/10.1016/0003-9861(77)90046-7
---------- CHICAGO ----------
Flawiá, M.M., Terenzi, H.F., Torres, H.N. "Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity" . Archives of Biochemistry and Biophysics 180, no. 2 (1977) : 334-342.
http://dx.doi.org/10.1016/0003-9861(77)90046-7
---------- MLA ----------
Flawiá, M.M., Terenzi, H.F., Torres, H.N. "Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity" . Archives of Biochemistry and Biophysics, vol. 180, no. 2, 1977, pp. 334-342.
http://dx.doi.org/10.1016/0003-9861(77)90046-7
---------- VANCOUVER ----------
Flawiá, M.M., Terenzi, H.F., Torres, H.N. Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity. Arch. Biochem. Biophys. 1977;180(2):334-342.
http://dx.doi.org/10.1016/0003-9861(77)90046-7