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Abstract:

Nucleotides and sugar nucleotides from coelomic oocytes of Bufo arenarum were extracted with trichloroacetic acid and analyzed by ion-exchange chromatography. The hypoxanthine and guanine were sequencially eluted from the column with water. Nucleotides and sugar nucleotides were eluted with a linear gradient of ammonium chloride. The first peak of ultraviolet adsorption eluted from the resin was a complex mixture of at least three substances. The main component was identified as cytidine diphosphocholine by chemical, enzymatic, and chromatographic analyses. Preliminary experiments suggest a possible role for this compound during oogenesis, since immature oocytes incubated in vitro with [14C]choline showed an active metabolism of this substance with rapid incorporation in choline phosphate, cytidine diphosphocholine, and lecithin. © 1977.

Registro:

Documento: Artículo
Título:Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes
Autor:Cantore, M.L.; De Recondo, M.E.F.; Recondo, E.F.
Filiación:Embriologia Animal y Biologia Molecular Departamentos de Ciencias Biologicas y Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:carbohydrate; guanine; hypoxanthine; nucleotide; radioisotope; choline c 14; development; egg; in vitro study; theoretical study; Animal; Bufo arenarum; Choline; Cytidine Diphosphate Choline; Female; Nucleoside Diphosphate Sugars; Nucleotides; Oocytes; Oogenesis; Ovum; Pentoses
Año:1977
Volumen:180
Número:2
Página de inicio:269
Página de fin:275
DOI: http://dx.doi.org/10.1016/0003-9861(77)90038-8
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:guanine, 69257-39-2, 73-40-5; hypoxanthine, 68-94-0; Choline, 62-49-7; Cytidine Diphosphate Choline, 987-78-0; Nucleoside Diphosphate Sugars; Nucleotides; Pentoses
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v180_n2_p269_Cantore

Referencias:

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Citas:

---------- APA ----------
Cantore, M.L., De Recondo, M.E.F. & Recondo, E.F. (1977) . Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes. Archives of Biochemistry and Biophysics, 180(2), 269-275.
http://dx.doi.org/10.1016/0003-9861(77)90038-8
---------- CHICAGO ----------
Cantore, M.L., De Recondo, M.E.F., Recondo, E.F. "Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes" . Archives of Biochemistry and Biophysics 180, no. 2 (1977) : 269-275.
http://dx.doi.org/10.1016/0003-9861(77)90038-8
---------- MLA ----------
Cantore, M.L., De Recondo, M.E.F., Recondo, E.F. "Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes" . Archives of Biochemistry and Biophysics, vol. 180, no. 2, 1977, pp. 269-275.
http://dx.doi.org/10.1016/0003-9861(77)90038-8
---------- VANCOUVER ----------
Cantore, M.L., De Recondo, M.E.F., Recondo, E.F. Nucleotides and sugar nucleotides in early development of Bufo arenarum. Coelomic oocytes. Arch. Biochem. Biophys. 1977;180(2):269-275.
http://dx.doi.org/10.1016/0003-9861(77)90038-8