Navegar

Colección

Datos Estadísticas

Artículo

El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Protein kinase and cyclic adenosine 3′,5′-monophosphate (cAMP) binding activities have been detected in cell extracts of the dimorphic fungus Mucor rouxii. The subcellular distribution of both activities indicates that most of the binding protein is in the high-speed supernatant (S100), while about 70% of the total protein kinase activity remains in particulate fractions. S100 preparations have been analyzed by diethylaminoethyl cellulose column chromatography. Binding activity can be resolved in two peaks (A and B) and protein kinase in three peaks (I, II, and III). Peaks I and II are casein dependent and insensitive to cAMP. Peak III utilizes histone as substrate and is activated (two- to fourfold) by cAMP. Theophylline strongly inhibits cAMP binding activity and mimics the effect of cAMP on cAMP-dependent protein kinase. The possible relationship between cAMP binding activity and cAMP-dependent protein kinase is suggested. © 1977.

Registro:

Documento: Artículo
Título:Multiple protein kinase activities in the dimorphic fungus Mucor rouxii. Comparison with a cyclic adenosine 3′,5′-monophosphate binding protein
Autor:Moreno, S.; Paveto, C.; Passeron, S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 4 Piso, Ciudad Universitaria, Nuñez, Buenos Aires, Argentina
Palabras clave:cyclic amp; protein kinase; in vitro study; microorganism; mucor rouxii; theoretical study; Cyclic AMP; Kinetics; Mucor; Protein Binding; Protein Kinases; Receptors, Cyclic AMP; Subcellular Fractions; Theophylline
Año:1977
Volumen:180
Número:2
Página de inicio:225
Página de fin:231
DOI: http://dx.doi.org/10.1016/0003-9861(77)90032-7
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:cyclic AMP, 60-92-4; protein kinase, 9026-43-1; Cyclic AMP, 60-92-4; Protein Kinases, EC 2.7.1.37; Receptors, Cyclic AMP; Theophylline, 58-55-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v180_n2_p225_Moreno

Referencias:

  • Bartnicki-García, Nickerson, Nutrition, growth, and morphogenesis of Mucor rouxii. (1962) J Bacteriol, 84, pp. 841-858
  • Bartnicki-García, Control of dimorphism in Mucor by hexoses: inhibition of hyphal morphogenesis. (1968) J Bacteriol, 96, pp. 1586-1594
  • Terenzi, Storck, (1969) J. Bacteriol, 97, pp. 1248-1261
  • Friedenthal, Epstein, Passeron, (1974) J. Gen. Microbiol, 82, pp. 15-24
  • Paveto, Epstein, Passeron, (1975) Arch. Biochem. Biophys, 169, pp. 449-457
  • Walsh, Krebs, (1973) The Enzymes, 3, pp. 555-581. , 3rd ed., P.D. Boyer, Academic Press, New York/London
  • Uno, Ishikawa, (1974) Biochim. Biophys. Acta, 334, pp. 354-360
  • Inoue, Yamamura, Nishizuka, (1973) Biochem. Biophys. Res. Commun, 50, pp. 228-236
  • Terenzi, Roselino, Passeron, (1971) Eur. J. Biochem, 18, pp. 342-350
  • Piras, Staneloni, Leiderman, Piras, (1972) FEBS Lett, 23, pp. 199-202
  • Gilman, (1970) Proc. Nat. Acad. Sci. USA, 67, pp. 305-312. , 1st ed
  • Lowry, Rosebrough, Farr, Randall, (1951) J. Biol. Chem, 193, pp. 265-275
  • Glynn, Chappell, (1964) Biochem. J, 90, pp. 147-149
  • Reimann, Walsh, Krebs, (1971) J. Biol. Chem, 246, pp. 1986-1995
  • Walsh, Ashby, González, Calkins, Fischer, Krebs, (1971) J. Biol. Chem, 246, pp. 1977-1985
  • Skala, Drummond, Hahn, (1974) Biochem. J, 138, pp. 195-199
  • Desjardins, Lue, Liew, Gornall, (1972) Canad. J. Biochem, 50, pp. 1249-1259
  • Desjardins, Mendelson, Anderson, (1975) Canad. J. Biochem, 53, pp. 591-598

Citas:

---------- APA ----------
Moreno, S., Paveto, C. & Passeron, S. (1977) . Multiple protein kinase activities in the dimorphic fungus Mucor rouxii. Comparison with a cyclic adenosine 3′,5′-monophosphate binding protein. Archives of Biochemistry and Biophysics, 180(2), 225-231.
http://dx.doi.org/10.1016/0003-9861(77)90032-7
---------- CHICAGO ----------
Moreno, S., Paveto, C., Passeron, S. "Multiple protein kinase activities in the dimorphic fungus Mucor rouxii. Comparison with a cyclic adenosine 3′,5′-monophosphate binding protein" . Archives of Biochemistry and Biophysics 180, no. 2 (1977) : 225-231.
http://dx.doi.org/10.1016/0003-9861(77)90032-7
---------- MLA ----------
Moreno, S., Paveto, C., Passeron, S. "Multiple protein kinase activities in the dimorphic fungus Mucor rouxii. Comparison with a cyclic adenosine 3′,5′-monophosphate binding protein" . Archives of Biochemistry and Biophysics, vol. 180, no. 2, 1977, pp. 225-231.
http://dx.doi.org/10.1016/0003-9861(77)90032-7
---------- VANCOUVER ----------
Moreno, S., Paveto, C., Passeron, S. Multiple protein kinase activities in the dimorphic fungus Mucor rouxii. Comparison with a cyclic adenosine 3′,5′-monophosphate binding protein. Arch. Biochem. Biophys. 1977;180(2):225-231.
http://dx.doi.org/10.1016/0003-9861(77)90032-7