The specificity of the transfer of monosaccharides from sugar nucleotides to dolichol monophosphate catalyzed by liver microsomes was studied. Besides uridine diphosphate glucose, uridine diphosphate-N-acetylglucosamine and guanosine diphosphate mannose were found to act as donors for the formation of the respective dolichol monophosphate sugars. Uridine diphosphate galactose and uridine diphosphate-N-acetylgalactosamine gave negative results. The optimal conditions for transfer from dolichol monophosphate glucose to endogenous acceptor was determined. Studies were carried out on the glucosylation of ceramide by brain extracts and of collagen by skin enzymes in order to find out if dolichol monophosphate glucose is an intermediate in these reactions. The results, while not definite, were not in favor of this assumption. © 1971.
Documento: | Artículo |
Título: | The role of dolichol monophosphate in sugar transfer |
Autor: | Behrens, N.H.; Parodi, A.J.; Leloir, L.F.; Krisman, C.R. |
Filiación: | Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina |
Palabras clave: | alkene; amino acid; bile acid; cerebroside; collagen; fatty acid; galactose; glucosamine; glucose; glycol; guanine nucleotide; hexosamine; hexose; mannose; nucleoside diphosphate sugar; nucleotide; organophosphorus compound; phosphoric acid; polymer; pyrimidine nucleotide; Surface Active Agents; surfactant; animal; article; brain; catalysis; drug stability; enzymology; liver microsome; metabolism; pH; rat; skin; solubility; temperature; thin layer chromatography; Alkenes; Amino Acids; Animal; Animals, Newborn; Bile Acids and Salts; Brain; Catalysis; Cerebrosides; Chromatography, Thin Layer; Collagen; Drug Stability; Fatty Acids; Galactose; Glucosamine; Glucose; Glycols; Guanine Nucleotides; Hexosamines; Hexoses; Hydrogen-Ion Concentration; Mannose; Microsomes, Liver; Nucleoside Diphosphate Sugars; Nucleotides; Organophosphorus Compounds; Phosphoric Acids; Polymers; Rats; Skin; Solubility; Surface-Active Agents; Temperature; Uracil Nucleotides |
Año: | 1971 |
Volumen: | 143 |
Número: | 2 |
Página de inicio: | 375 |
Página de fin: | 383 |
DOI: | http://dx.doi.org/10.1016/0003-9861(71)90224-4 |
Título revista: | Archives of Biochemistry and Biophysics |
Título revista abreviado: | Arch. Biochem. Biophys. |
ISSN: | 00039861 |
CODEN: | ABBIA |
CAS: | amino acid, 65072-01-7; collagen, 9007-34-5; galactose, 26566-61-0, 50855-33-9, 59-23-4; glucosamine, 3416-24-8, 4607-22-1; glucose, 50-99-7, 84778-64-3; hexose, 93780-23-5; mannose, 31103-86-3, 3458-28-4; phosphoric acid, 7664-38-2; Alkenes; Amino Acids; Bile Acids and Salts; Cerebrosides; Collagen, 9007-34-5; Fatty Acids; Galactose, 26566-61-0; Glucosamine, 3416-24-8; Glucose, 50-99-7; Glycols; Guanine Nucleotides; Hexosamines; Hexoses; Mannose, 31103-86-3; Nucleoside Diphosphate Sugars; Nucleotides; Organophosphorus Compounds; Phosphoric Acids; Polymers; Surface-Active Agents; Uracil Nucleotides |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v143_n2_p375_Behrens |