Artículo
Abstract:
High molecular weight glycogen has been prepared in vitro with muscle glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and liver or muscle branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) with uridine diphosphate glucose as glucose donor. The product obtained with a fresh synthetase preparation had a relatively low molecular weight like native muscle glycogen. The product synthesized with an aged synthetase preparation had a very high molecular weight similar to that of native liver glycogen. Ammonium sulfate and other salts inhibited the formation of high molecular weight glycogen by the aged synthetase preparation but not the total synhesis of the polysaccharide. The effect of the salts on the molecular weight of the product synthesized by the liver synthetase was much smaller. The changes in the molecular weight distribution of glycogen during synthesis with fresh, aged, and aged plus ammonium sulfate glycogen synthetases were studied. The results obtained are discussed. © 1970.
Registro:
| Documento: | Artículo |
| Título: | In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase |
| Autor: | Krisman, C.R.; Parodi, A.J. |
| Filiación: | Institute de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires 28, Argentina |
| Palabras clave: | ammonium derivative; glucose; glucosyltransferase; glycogen; phosphate; pyrimidine nucleotide; sodium chloride; sulfate; animal; article; biosynthesis; enzymology; glycogen liver level; liver; molecular weight; muscle; rat; time; Ammonium Compounds; Animal; Glucose; Glucosyltransferases; Glycogen; Liver; Liver Glycogen; Molecular Weight; Muscles; Phosphates; Rats; Sodium Chloride; Sulfates; Time Factors; Uracil Nucleotides |
| Año: | 1970 |
| Volumen: | 141 |
| Número: | 1 |
| Página de inicio: | 228 |
| Página de fin: | 235 |
| DOI: | http://dx.doi.org/10.1016/0003-9861(70)90126-8 |
| Título revista: | Archives of Biochemistry and Biophysics |
| Título revista abreviado: | Arch. Biochem. Biophys. |
| ISSN: | 00039861 |
| CODEN: | ABBIA |
| CAS: | glucose, 50-99-7, 84778-64-3; glucosyltransferase, 9031-48-5; glycogen, 9005-79-2; phosphate, 14066-19-4, 14265-44-2; sodium chloride, 7647-14-5; sulfate, 14808-79-8; Ammonium Compounds; Glucose, 50-99-7; Glucosyltransferases, EC 2.4.1.-; Glycogen, 9005-79-2; Liver Glycogen; Phosphates; Sodium Chloride, 7647-14-5; Sulfates; Uracil Nucleotides |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v141_n1_p228_Krisman |
Referencias:
- Bueding, Orrell, Sidbury, (1964) Ciba Foundation Symposium on Control of Glycogen Metabolism, p. 387. , W.J. Whelan, M.P. Cameron, Churchill, London
- Orrell, Bueding, Reissig, (1964) Ciba Foundation Symposium on Control of Glycogen Metabolism, p. 29. , W.J. Whelan, M.P. Cameron, Churchill, London
- Parodi, Krisman, Leloir, Mordoh, (1967) Arch. Biochem. Biophys, 121, p. 769
- Barber, Harris, Anderson, (1966) Nat. Cancer Inst. Monogr, 21, p. 287
- Mordoh, Krisman, Leloir, (1966) Arch. Biochem. Biophys, 113, p. 265
- Parodi, A. J., unpublished results; Parodi, Mordoh, Krisman, Leloir, (1969) Arch. Biochem. Biophys, 132, p. 111
- Mordoh, Leloir, Krisman, (1965) Proc. Nat. Acad. Sci. U. S. A, 53, p. 86
- Parodi, Krisman, Mordoh, (1970) Arch. Biochem. Biophys, 141, p. 219
- Krisman, (1962) Biochim. Biophys. Acta, 65, p. 307
- Krisman, (1962) Anal. Biochem, 4, p. 17
Citas:
---------- APA ----------
Krisman, C.R. & Parodi, A.J. (1970) . In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase. Archives of Biochemistry and Biophysics, 141(1), 228-235.http://dx.doi.org/10.1016/0003-9861(70)90126-8
---------- CHICAGO ----------
Krisman, C.R., Parodi, A.J. "In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase" . Archives of Biochemistry and Biophysics 141, no. 1 (1970) : 228-235.http://dx.doi.org/10.1016/0003-9861(70)90126-8
---------- MLA ----------
Krisman, C.R., Parodi, A.J. "In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase" . Archives of Biochemistry and Biophysics, vol. 141, no. 1, 1970, pp. 228-235.http://dx.doi.org/10.1016/0003-9861(70)90126-8
---------- VANCOUVER ----------
Krisman, C.R., Parodi, A.J. In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase. Arch. Biochem. Biophys. 1970;141(1):228-235.http://dx.doi.org/10.1016/0003-9861(70)90126-8
