High molecular weight glycogen has been prepared in vitro with liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) and uridine diphosphate glucose as glucose donor. The product obtained did not differ significantly from the native glycogen as judged by iodine spectrum, sedimentation coefficient in sucrose gradients, and by the effect of treatment with acid or alkali. Glycogen obtained from uridine diphosphate glucose differed from that prepared with glucose 1-phosphate as glucosyl donor. © 1969.
Documento: | Artículo |
Título: | In vitro synthesis of particulate glycogen from uridine diphosphate glucose |
Autor: | Parodi, A.J.; Mordoh, J.; Krisman, C.R.; Leloir, L.F. |
Filiación: | Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina |
Palabras clave: | glucose; glucosyltransferase; glycogen; hydrochloric acid; iodine; pyrimidine nucleotide; sodium hydroxide; animal; article; biosynthesis; density gradient centrifugation; enzymology; liver; metabolism; molecular weight; pH; rat; spectroscopy; Animal; Centrifugation, Density Gradient; Glucose; Glucosyltransferases; Glycogen; Hydrochloric Acid; Hydrogen-Ion Concentration; Iodine; Liver; Molecular Weight; Rats; Sodium Hydroxide; Spectrum Analysis; Uracil Nucleotides |
Año: | 1969 |
Volumen: | 132 |
Número: | 1 |
Página de inicio: | 111 |
Página de fin: | 117 |
DOI: | http://dx.doi.org/10.1016/0003-9861(69)90342-7 |
Título revista: | Archives of Biochemistry and Biophysics |
Título revista abreviado: | Arch. Biochem. Biophys. |
ISSN: | 00039861 |
CODEN: | ABBIA |
CAS: | glucose, 50-99-7, 84778-64-3; glucosyltransferase, 9031-48-5; glycogen, 9005-79-2; hydrochloric acid, 7647-01-0; iodine, 7553-56-2; sodium hydroxide, 1310-73-2; Glucose, 50-99-7; Glucosyltransferases, EC 2.4.1.-; Glycogen, 9005-79-2; Hydrochloric Acid, 7647-01-0; Iodine, 7553-56-2; Sodium Hydroxide, 1310-73-2; Uracil Nucleotides |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v132_n1_p111_Parodi |