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Abstract:

High molecular weight glycogen has been prepared in vitro with liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) and uridine diphosphate glucose as glucose donor. The product obtained did not differ significantly from the native glycogen as judged by iodine spectrum, sedimentation coefficient in sucrose gradients, and by the effect of treatment with acid or alkali. Glycogen obtained from uridine diphosphate glucose differed from that prepared with glucose 1-phosphate as glucosyl donor. © 1969.

Registro:

Documento: Artículo
Título:In vitro synthesis of particulate glycogen from uridine diphosphate glucose
Autor:Parodi, A.J.; Mordoh, J.; Krisman, C.R.; Leloir, L.F.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina
Palabras clave:glucose; glucosyltransferase; glycogen; hydrochloric acid; iodine; pyrimidine nucleotide; sodium hydroxide; animal; article; biosynthesis; density gradient centrifugation; enzymology; liver; metabolism; molecular weight; pH; rat; spectroscopy; Animal; Centrifugation, Density Gradient; Glucose; Glucosyltransferases; Glycogen; Hydrochloric Acid; Hydrogen-Ion Concentration; Iodine; Liver; Molecular Weight; Rats; Sodium Hydroxide; Spectrum Analysis; Uracil Nucleotides
Año:1969
Volumen:132
Número:1
Página de inicio:111
Página de fin:117
DOI: http://dx.doi.org/10.1016/0003-9861(69)90342-7
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:glucose, 50-99-7, 84778-64-3; glucosyltransferase, 9031-48-5; glycogen, 9005-79-2; hydrochloric acid, 7647-01-0; iodine, 7553-56-2; sodium hydroxide, 1310-73-2; Glucose, 50-99-7; Glucosyltransferases, EC 2.4.1.-; Glycogen, 9005-79-2; Hydrochloric Acid, 7647-01-0; Iodine, 7553-56-2; Sodium Hydroxide, 1310-73-2; Uracil Nucleotides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v132_n1_p111_Parodi

Citas:

---------- APA ----------
Parodi, A.J., Mordoh, J., Krisman, C.R. & Leloir, L.F. (1969) . In vitro synthesis of particulate glycogen from uridine diphosphate glucose. Archives of Biochemistry and Biophysics, 132(1), 111-117.
http://dx.doi.org/10.1016/0003-9861(69)90342-7
---------- CHICAGO ----------
Parodi, A.J., Mordoh, J., Krisman, C.R., Leloir, L.F. "In vitro synthesis of particulate glycogen from uridine diphosphate glucose" . Archives of Biochemistry and Biophysics 132, no. 1 (1969) : 111-117.
http://dx.doi.org/10.1016/0003-9861(69)90342-7
---------- MLA ----------
Parodi, A.J., Mordoh, J., Krisman, C.R., Leloir, L.F. "In vitro synthesis of particulate glycogen from uridine diphosphate glucose" . Archives of Biochemistry and Biophysics, vol. 132, no. 1, 1969, pp. 111-117.
http://dx.doi.org/10.1016/0003-9861(69)90342-7
---------- VANCOUVER ----------
Parodi, A.J., Mordoh, J., Krisman, C.R., Leloir, L.F. In vitro synthesis of particulate glycogen from uridine diphosphate glucose. Arch. Biochem. Biophys. 1969;132(1):111-117.
http://dx.doi.org/10.1016/0003-9861(69)90342-7