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Abstract:

The conditions required for in vitro complementation between a pair ot mutants deficient in aspartic transcarbamylase were determined. It was observed that complementation in vitro and in vivo produces varieties of aspartic transcarbamylase which are distinguishable from the wild type enzyme and from each other on the basis of thermal stability and other criteria. The significance of these findings with reference to the molecular mechanism of complementation is discussed. © 1966.

Registro:

Documento: Artículo
Título:In vivo and in vitro complementation between aspartic transcarbamylase mutants of neurospora
Autor:Issaly, A.S.; Reissig, J.L.
Filiación:Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Perú, 222, Argentina
Instituto Nacional de Microbiología, Avda. Vélez Sarsfield, 563 Buenos Aires, Argentina
Palabras clave:transferase; article; biosynthesis; enzymology; heat; molecular biology; mutation; Neurospora; Heat; Molecular Biology; Mutation; Neurospora; Transferases
Año:1966
Volumen:116
Número:C
Página de inicio:44
Página de fin:51
DOI: http://dx.doi.org/10.1016/0003-9861(66)90010-5
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:transferase, 9047-61-4; Transferases, EC 2.
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v116_nC_p44_Issaly

Citas:

---------- APA ----------
Issaly, A.S. & Reissig, J.L. (1966) . In vivo and in vitro complementation between aspartic transcarbamylase mutants of neurospora. Archives of Biochemistry and Biophysics, 116(C), 44-51.
http://dx.doi.org/10.1016/0003-9861(66)90010-5
---------- CHICAGO ----------
Issaly, A.S., Reissig, J.L. "In vivo and in vitro complementation between aspartic transcarbamylase mutants of neurospora" . Archives of Biochemistry and Biophysics 116, no. C (1966) : 44-51.
http://dx.doi.org/10.1016/0003-9861(66)90010-5
---------- MLA ----------
Issaly, A.S., Reissig, J.L. "In vivo and in vitro complementation between aspartic transcarbamylase mutants of neurospora" . Archives of Biochemistry and Biophysics, vol. 116, no. C, 1966, pp. 44-51.
http://dx.doi.org/10.1016/0003-9861(66)90010-5
---------- VANCOUVER ----------
Issaly, A.S., Reissig, J.L. In vivo and in vitro complementation between aspartic transcarbamylase mutants of neurospora. Arch. Biochem. Biophys. 1966;116(C):44-51.
http://dx.doi.org/10.1016/0003-9861(66)90010-5