A new enzyme has been found in yeast, which catalyzes the hydrolysis of guanosine diphosphate glucose to guanosine diphosphate and glucose. The enzyme was purified about 130-fold and its kinetic properties were studied. The enzymic activity seems to be strictly specific for guanosine diphosphate glucose, with a Km of 0.23 mm. The enzyme is active over a wide range of pH values, from 5 to 9. Guanosine diphosphate inhibits competitively and Mg++ partially relieves the inhibition. The enzyme appears to exist in soluble form in the cytoplasm. The possible significance of guanosine diphosphate glucose glucohydrolase and of sugar nucleotide phosphorylase in the control of the intracellular concentration of guanosine diphosphate glucose and guanosine diphosphate mannose is discussed. © 1966.
Documento: | Artículo |
Título: | Guanosine diphosphate d-glucose glucohydrolase |
Autor: | Sonnino, S.; Carminatti, H.; Cabib, E. |
Filiación: | Instituto de Investigaciones Bioquímicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina |
Palabras clave: | glucose; glycosidase; guanine nucleotide; magnesium; mannose; article; chromatography; electrophoresis; enzymology; gel chromatography; kinetics; metabolism; Saccharomyces; Chromatography; Chromatography, Gel; Electrophoresis; Glucose; Glycoside Hydrolases; Guanine Nucleotides; Kinetics; Magnesium; Mannose; Saccharomyces |
Año: | 1966 |
Volumen: | 116 |
Número: | C |
Página de inicio: | 26 |
Página de fin: | 33 |
DOI: | http://dx.doi.org/10.1016/0003-9861(66)90007-5 |
Título revista: | Archives of Biochemistry and Biophysics |
Título revista abreviado: | Arch. Biochem. Biophys. |
ISSN: | 00039861 |
CODEN: | ABBIA |
CAS: | glucose, 50-99-7, 84778-64-3; glycosidase, 9032-92-2; magnesium, 7439-95-4; mannose, 31103-86-3, 3458-28-4; Glucose, 50-99-7; Glycoside Hydrolases, EC 3.2.1.; Guanine Nucleotides; Magnesium, 7439-95-4; Mannose, 31103-86-3 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v116_nC_p26_Sonnino |