Artículo
Abstract:
The mechanism of sucrose-starch transformation was studied in corn endosperm. Preparations of endosperm which contain the total protein and starch granules catalyze an incorporation of radioactivity from sucrose-C14 to the granule in presence of ADP or UDP, ADP being more effective. No transfer of glucose-C14 from glucose-1-P to the granule was observed unless Mg++ and ATP were present. The Km values of the UDP-glucose-fructose glucosyl transferase for ADP, UDP, ADP-glucose, and UDP-glucose and their reciprocal inhibitions were measured From all these results the following two sequences for the glucose incorporation into the granule were proposed: (a) SucroseαADP-glucose (or UDP-glucose)→starch (b) SucroseαUDP-glucoseαglucose-1-PαADP-glucose→starch. The enzymes which catalyze all these steps could be demonstrated in corn endosperm. © 1964 Academic Press Inc. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Mechanism of glucose transfer from sucrose into the starch granule of sweet corn |
| Autor: | Rongine De Fekete, M.A.; Cardini, C.E. |
| Filiación: | Instituto de Investigaciones Bioquímicas Fundación Campomar, Obligado, 2490 Buenos Aires (28), Argentina Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina |
| Palabras clave: | adenine nucleotide; adenosine triphosphate; carbon; glucose; magnesium; phosphotransferase; pyrimidine nucleotide; starch; sucrose; article; carbohydrate metabolism; cereal; EXPERIMENTAL LAB STUDY; grain; research; ADENINE NUCLEOTIDES; ADENOSINE TRIPHOSPHATE; CARBOHYDRATE METABOLISM; CARBON ISOTOPES; EXPERIMENTAL LAB STUDY; GLUCOSE; GRAIN; MAGNESIUM; PHOSPHOTRANSFERASES; STARCH; SUCROSE; URACIL NUCLEOTIDES; Adenine Nucleotides; Adenosine Triphosphate; Carbohydrate Metabolism; Carbon Isotopes; Cereals; Glucose; Magnesium; Phosphotransferases; Research; Starch; Sucrose; Uracil Nucleotides |
| Año: | 1964 |
| Volumen: | 104 |
| Número: | 1 |
| Página de inicio: | 173 |
| Página de fin: | 184 |
| DOI: | http://dx.doi.org/10.1016/S0003-9861(64)80052-7 |
| Título revista: | Archives of Biochemistry and Biophysics |
| Título revista abreviado: | Arch. Biochem. Biophys. |
| ISSN: | 00039861 |
| CODEN: | ABBIA |
| CAS: | adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; carbon, 7440-44-0; glucose, 50-99-7, 84778-64-3; magnesium, 7439-95-4; phosphotransferase, 9031-09-8, 9031-44-1; starch, 9005-25-8, 9005-84-9; sucrose, 122880-25-5, 57-50-1; Adenine Nucleotides; Adenosine Triphosphate, 56-65-5; Carbon Isotopes; Glucose, 50-99-7; Magnesium, 7439-95-4; Phosphotransferases, 2.7.-; Starch, 9005-25-8; Sucrose, 57-50-1; Uracil Nucleotides |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v104_n1_p173_RongineDeFekete |
Citas:
---------- APA ----------
Rongine De Fekete, M.A. & Cardini, C.E. (1964) . Mechanism of glucose transfer from sucrose into the starch granule of sweet corn. Archives of Biochemistry and Biophysics, 104(1), 173-184.http://dx.doi.org/10.1016/S0003-9861(64)80052-7
---------- CHICAGO ----------
Rongine De Fekete, M.A., Cardini, C.E. "Mechanism of glucose transfer from sucrose into the starch granule of sweet corn" . Archives of Biochemistry and Biophysics 104, no. 1 (1964) : 173-184.http://dx.doi.org/10.1016/S0003-9861(64)80052-7
---------- MLA ----------
Rongine De Fekete, M.A., Cardini, C.E. "Mechanism of glucose transfer from sucrose into the starch granule of sweet corn" . Archives of Biochemistry and Biophysics, vol. 104, no. 1, 1964, pp. 173-184.http://dx.doi.org/10.1016/S0003-9861(64)80052-7
---------- VANCOUVER ----------
Rongine De Fekete, M.A., Cardini, C.E. Mechanism of glucose transfer from sucrose into the starch granule of sweet corn. Arch. Biochem. Biophys. 1964;104(1):173-184.http://dx.doi.org/10.1016/S0003-9861(64)80052-7
