Artículo
Abstract:
Escherichia coli recombinant strains bearing the thr operon have been previously selected for threonine production and phenotypically classified according to antibiotic resistance properties (Nudel et al. 1987). Further analysis of those strains permitted the isolation and restriction mapping of two different plasmids of 13 kb and 18.6 kb. The smaller one, which expressed tetracycline resistance gave better results on threonine accumulation but it was rather unstable when grown without antibiotic pressure. Therefore, other hosts were transformed with those plasmids to improve stability. A threonine-auxotrophic strain was a better host for plasmid maintenance and expression of thr operon. Host influence in plasmid-mediated threonine production was studied in terms of specific yields (the ratios of threonine accumulated to biomass values) and of plasmid maintenance (percent of AprTcr clones after cultivation in non selective media). We also determined that semisynthetic media of defined composition were better than rich media for threonine expression, due to feed-back controls exerted by undesired catabolites accumulated in complex media. © 1989 Kluwer Academic Publishers.
Registro:
| Documento: | Artículo |
| Título: | Stability of Escherichia coli strains harboring recombinant plasmids for L-threonine production |
| Autor: | Nudel, B.C.; Pueyo, M.G.; Judewicz, N.D.; Guilietti, A.M. |
| Filiación: | Biotecnologia y Microbiologia Industrial, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956, Buenos Aires, 1113, Argentina Instituto de Investigaciones en Ingeniería Genética y Biologia Molecular (INGEBI), Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina |
| Palabras clave: | host-plasmid interaction; threonine production; escherichia coli; nonhuman; plasmid stability; recombinant plasmid; Culture Media; Escherichia coli; Kinetics; Phenotype; Plasmids; Recombination, Genetic; Restriction Mapping; Threonine; Transformation, Bacterial |
| Año: | 1989 |
| Volumen: | 56 |
| Número: | 3 |
| Página de inicio: | 273 |
| Página de fin: | 282 |
| DOI: | http://dx.doi.org/10.1007/BF00418939 |
| Título revista: | Antonie van Leeuwenhoek |
| Título revista abreviado: | Antonie van Leeuwenhoek |
| ISSN: | 00036072 |
| CODEN: | ALJMA |
| CAS: | Culture Media; Plasmids; Threonine, 72-19-5 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00036072_v56_n3_p273_Nudel |
Referencias:
- Dagert, M., Ehrlich, S.D., Prolonged incubation in calcium chloride improves the competence of E. coli (1979) Gene, 6, pp. 23-28
- Imanaka, T., Aiba, S., A perspective on the application of genetic engineering: stability of recombinant plasmid (1981) Ann. N.Y. Acad. Sci., 369, pp. 1-14
- Joshi, S., Yamazaki, H., Stability of plasmid pBR322 in E. coli immobilized on cotton cloth during use as resident inoculum (1987) Biotech. Lett., 9, pp. 825-830
- Maniatis T, Fritsch EF & Sambrook J (1982) Molecular Cloning. A laboratory manual. Cold Spring Harbor Laboratory; Miwa K, Tsuchida T, Kurahashi O, Nakamori S, Sano K & Momose H (1980) Threonine production by fermentation. U.K. Patent Application 2049670; Miwa, K., Nakamori, S., Sano, K., Momose, H., Stability of recombinant plasmids carrying the threonine operon in E. coli (1984) Agric. Biol. Chem., 48, pp. 2233-2237
- Momose H (1983) New genetic approaches to amino-acid producing strains. In: Developments in Industrial Microbiol. 24: 109–119; Noack, D., Roth, M., Geuther, R., Müller, G., Undisz, K., Hoffmeier, Gáspár, S., Maintenance and genetic stability of vector plasmids pBR322 and pBR325 in E. coli K12 strains grown in a chemostat (1981) Molec. Gen. Genet., 184, pp. 121-124
- Nudel, B.C., Corradini, C., Fraile, E., Giulietti, A.M., Plasmid maintenance and threonine production by recombinant E. coli strains (1987) Biotech. Lett., 9, pp. 77-82
- Robinson, M., Marquardt, R., Theu, J., McChesney, J., Neatherway, H., Wöhner, G., Deger, H.M., Prave, P., Cloning of the gene coding for aromatic amino acid aminotranferase from an E. coli B Strain (1987) Biotech. Lett., 9, pp. 673-679
- Ryu, D.D.Y., Siegel, R., Scale-up of fermentation processes using recombinant organisms (1986) Ann. N.Y. Acad. Sci., 469, pp. 73-82
- Summers, D.K., Sherrat, D.J., Multimerization of high copy number plasmids causes instability: ColE1 encodes a determinant essential for plasmid monomerization and stability (1984) Cell, 36, pp. 1097-1103
- Yukawa, H., Kurusu, Y., Shimazu, M., Yamagata, H., Teresawa, M., Stabilization of an E. coli plasmid by a mini-F fragment of DNA (1988) J. Ind. Microbiol., 2, pp. 323-328
Citas:
---------- APA ----------
Nudel, B.C., Pueyo, M.G., Judewicz, N.D. & Guilietti, A.M. (1989) . Stability of Escherichia coli strains harboring recombinant plasmids for L-threonine production. Antonie van Leeuwenhoek, 56(3), 273-282.http://dx.doi.org/10.1007/BF00418939
---------- CHICAGO ----------
Nudel, B.C., Pueyo, M.G., Judewicz, N.D., Guilietti, A.M. "Stability of Escherichia coli strains harboring recombinant plasmids for L-threonine production" . Antonie van Leeuwenhoek 56, no. 3 (1989) : 273-282.http://dx.doi.org/10.1007/BF00418939
---------- MLA ----------
Nudel, B.C., Pueyo, M.G., Judewicz, N.D., Guilietti, A.M. "Stability of Escherichia coli strains harboring recombinant plasmids for L-threonine production" . Antonie van Leeuwenhoek, vol. 56, no. 3, 1989, pp. 273-282.http://dx.doi.org/10.1007/BF00418939
---------- VANCOUVER ----------
Nudel, B.C., Pueyo, M.G., Judewicz, N.D., Guilietti, A.M. Stability of Escherichia coli strains harboring recombinant plasmids for L-threonine production. Antonie van Leeuwenhoek. 1989;56(3):273-282.http://dx.doi.org/10.1007/BF00418939
