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Samples of peripheral blood were collected once from non-smoking women who were healthy (controls) and twice (immediately before and immediately after 7 days of treatment with metronidazole at 500 mg/day) from non-smoking women infected with Trichomonas vaginalis. Lymphocyte cultures were prepared and used, in toxicogenetic studies, to determine the frequency of sister-chromatid exchange (SCE), the mitotic index (MI), and the replication index (RI) for each sample. MTZ treatment of the infected women led to an increase in the frequency of SCE (P < 0.001), a decrease in the MI (P < 0.003), and a modification in the kinetics of cell proliferation, with a decrease in the RI (P < 0.0006). The differences seen between the results for the controls and those for the infected women, before and after MTZ treatment, may be attributed to the presence of the parasite, to the treatment itself, and/or to variation in the host's response to infection with T. vaginalis.


Documento: Artículo
Título:Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis
Autor:Carballo, M.A.; Palermo, A.M.; Mudry, M.D.
Filiación:Citogenetica y Genet. Toxicologica, Depto. de Bioquim. Clin., Universidad de Buenos Aires, Junín 956, (1113), Buenos Aires, Argentina
Inst. Invest. Cie. Tec. de las FFAA, Juan Bautista de la Salle 4397, (1603), Buenos Aires, Argentina
Grupo de Invest. en Biol. Evolutiva, Depto. de Ecologia, Genet. y Evol., Pabellón 2, Lab. 46-47, (C1428EHA), Buenos Aires, Argentina
Palabras clave:metronidazole; adult; article; blood sampling; cell division; clinical article; clinical trial; confidence interval; controlled clinical trial; controlled study; drug effect; drug screening; female; genotoxicity; human; human cell; immune response; lymphocyte culture; lymphocyte proliferation; mitosis index; parameter; sister chromatid exchange; Trichomonas vaginalis; trichomoniasis; Adult; Antitrichomonal Agents; Cell Division; Cells, Cultured; Female; Humans; Lymphocytes; Metronidazole; Mitotic Index; Sister Chromatid Exchange; Trichomonas Vaginitis; Protozoa; Trichomonas vaginalis
Página de inicio:139
Página de fin:147
Título revista:Annals of Tropical Medicine and Parasitology
Título revista abreviado:Ann. Trop. Med. Parasitol.
CAS:metronidazole, 39322-38-8, 443-48-1; Antitrichomonal Agents; Metronidazole, 443-48-1


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---------- APA ----------
Carballo, M.A., Palermo, A.M. & Mudry, M.D. (2004) . Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis. Annals of Tropical Medicine and Parasitology, 98(2), 139-147.
---------- CHICAGO ----------
Carballo, M.A., Palermo, A.M., Mudry, M.D. "Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis" . Annals of Tropical Medicine and Parasitology 98, no. 2 (2004) : 139-147.
---------- MLA ----------
Carballo, M.A., Palermo, A.M., Mudry, M.D. "Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis" . Annals of Tropical Medicine and Parasitology, vol. 98, no. 2, 2004, pp. 139-147.
---------- VANCOUVER ----------
Carballo, M.A., Palermo, A.M., Mudry, M.D. Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomonas vaginalis. Ann. Trop. Med. Parasitol. 2004;98(2):139-147.