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Abstract:

We describe a method based on a caged fluorescent molecule that can act as a chemical two-photon probe. It is composed of an organic fluorophore and a ruthenium-bipyridine complex that acts as a photoremovable quencher. For the fluorophore to be emissive, two independent photons must act on the molecule: the first photon frees the fluorescent ligand from the Ru complex and the second photon excites the fluorescence. In this two-photon regime, the emission is not proportional to the excitation intensity but rather to its second power, as in traditional two-photon systems based on ultrashort pulsed high-power lasers. This quadratic relationship implies a much higher spatial precision on the z-axis when the probe is used in a microscopy technique. The chemical nature of the two-photon excitation mechanism allows the use of inexpensive low-power lasers. © 2015 American Chemical Society.

Registro:

Documento: Artículo
Título:Chemical two-photon fluorescence
Autor:Carrone, G.; Etchenique, R.
Filiación:Departamento de Química Inorgánica, Analítica y Química Física, Universidad de Buenos Aires, Ciudad Universitaria Pabellón 2, AR1428EHA Buenos Aires, Argentina
Palabras clave:Fluorescence; Fluorophores; High power lasers; Molecules; Probes; Pulsed lasers; Ruthenium compounds; Ultrashort pulses; Excitation intensity; Fluorescent molecules; Microscopy technique; Organic fluorophores; Ruthenium-bipyridine complexes; Two photon fluorescence; Two-photon excitations; Ultra-short pulsed; Photons
Año:2015
Volumen:87
Número:8
Página de inicio:4363
Página de fin:4369
DOI: http://dx.doi.org/10.1021/acs.analchem.5b00131
Título revista:Analytical Chemistry
Título revista abreviado:Anal. Chem.
ISSN:00032700
CODEN:ANCHA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v87_n8_p4363_Carrone

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Citas:

---------- APA ----------
Carrone, G. & Etchenique, R. (2015) . Chemical two-photon fluorescence. Analytical Chemistry, 87(8), 4363-4369.
http://dx.doi.org/10.1021/acs.analchem.5b00131
---------- CHICAGO ----------
Carrone, G., Etchenique, R. "Chemical two-photon fluorescence" . Analytical Chemistry 87, no. 8 (2015) : 4363-4369.
http://dx.doi.org/10.1021/acs.analchem.5b00131
---------- MLA ----------
Carrone, G., Etchenique, R. "Chemical two-photon fluorescence" . Analytical Chemistry, vol. 87, no. 8, 2015, pp. 4363-4369.
http://dx.doi.org/10.1021/acs.analchem.5b00131
---------- VANCOUVER ----------
Carrone, G., Etchenique, R. Chemical two-photon fluorescence. Anal. Chem. 2015;87(8):4363-4369.
http://dx.doi.org/10.1021/acs.analchem.5b00131