Navegar

Colección

Datos Estadísticas

Artículo

El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

High potential purified Trametes trogii laccase has been deposited in mono- and multilayer thin films on gold surfaces by layer-by-layer electrostatic adsorption self-assembly. The osmium bipyridil redox relay sites on polycation poly(allylamine) backbone efficiently work as a molecular "wire" in oxygen cathodes for biofuel cells. X-ray photoelectron spectroscopy of Cu 2p3/2 and Os 4f signals provided chemical information on the enzyme and redox mediator surface concentrations after different adsorption steps. The electrical charge involved in oxidation-reduction cycles of the osmium sites, the ellipsometric enzyme film thickness, and the mass uptake from quartz crystal microbalance experiments, correlate with the XPS surface concentration, which provides unique evidence on the chemical identity of the composition in the topmost layers. XPS is shown to be an important analytical tool to investigate stratified copper and osmium distribution in LbL thin films relevant to biosensors and biofuel cells. (Chemical Equation Presented). © 2014 American Chemical Society.

Registro:

Documento: Artículo
Título:XPS analysis of enzyme and mediator at the surface of a layer-by-layer self-assembled wired enzyme electrode
Autor:Scodeller, P.; Williams, F.J.; Calvo, E.J.
Filiación:INQUIMAE, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, AR-1428, Argentina
Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Rd, San Diego, CA 92037, United States
Palabras clave:Adsorption; Biofuels; Biological fuel cells; Electrodes; Enzyme electrodes; Enzymes; Film preparation; Gold deposits; Molecular oxygen; Multilayer films; Multilayers; Osmium; Quartz; Self assembly; Chemical equations; Chemical identity; Chemical information; Electrical charges; Electrostatic adsorption; Mono- and multilayer; Oxidation-reduction cycle; Surface concentration; X ray photoelectron spectroscopy; enzymes, immobilized; Laccase; chemistry; electrodes; enzymology; methods; Oxidation-Reduction; Photoelectron Spectroscopy; Trametes; Electrodes; Enzymes, Immobilized; Laccase; Oxidation-Reduction; Photoelectron Spectroscopy; Trametes
Año:2014
Volumen:86
Número:24
Página de inicio:12180
Página de fin:12184
DOI: http://dx.doi.org/10.1021/ac503147c
Título revista:Analytical Chemistry
Título revista abreviado:Anal. Chem.
ISSN:00032700
CODEN:ANCHA
CAS:Enzymes, Immobilized; Laccase
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v86_n24_p12180_Scodeller

Referencias:

  • Solomon, E.I., Baldwin, M.J., Lowery, M.D., (1992) Chem. Rev., 92, pp. 521-542
  • Solomon, E.I., Augustine, A.J., Yoon, J., (2008) Dalton Trans., pp. 3921-3932
  • Solomon, E.I., Sundaram, U.M., Machonkin, T.E., (1996) Chem. Rev., 96, pp. 2563-2605
  • Tarasevich, M.R., Bogdanovskaya, V.A., Kuznetsova, L.N., (2001) Russ. J. Electrochem., 37, pp. 833-837
  • Palmore, G.T.R., Kim, H.H., (1999) J. Electroanal. Chem., 464, pp. 110-117
  • Chen, T., Barton, S.C., Binyamin, G., Gao, Z.Q., Zhang, Y.C., Kim, H.H., Heller, A., (2001) J. Am. Chem. Soc., 123, pp. 8630-8631
  • Calabrese Barton, S., Kim, H.H., Binyamin, G., Zhang, Y., Heller, A., (2001) J. Am. Chem. Soc., 123, pp. 5802-5803
  • Salaj-Kosla, U., Poeller, S., Schuhmann, W., Shleev, S., Magner, E., (2013) Bioelectrochemistry, 91, pp. 15-20
  • Shleev, S., Jarosz-Wilkolazka, A., Khalunina, A., Morozova, O., Yaropolov, A., Ruzgas, T., Gorton, L., (2005) Bioelectrochemistry, 67, pp. 115-124
  • Shleev, S., Pita, M., Yaropolov, A.I., Ruzgas, T., Gorton, L., (2006) Electroanalysis, 18, pp. 1901-1908
  • Shleev, S., Tkac, J., Christenson, A., Ruzgas, T., Yaropolov, A.I., Whittaker, J.W., Gorton, L., (2005) Biosens. Bioelectron., 20, pp. 2517-2554
  • Salaj-Kosla, U., Poeller, S., Schuhmann, W., Shleev, S., Magner, E., (2013) Bioelectrochemistry, 91, pp. 15-20
  • Climent, V., Zhang, J., Friis, E.P., Østergaard, L.H., Ulstrup, J., (2012) J. Phys. Chem. C, 116, pp. 1232-1243
  • Pita, M., Gutierrez-Sanchez, C., Olea, D., Velez, M., Garcia-Diego, C., Shleev, S., Fernandez, V.M., De Lacey, A.L., (2011) J. Phys. Chem. C, 115, pp. 13420-13428
  • Blanford, C.F., Foster, C.E., Heath, R.S., Armstrong, F.A., (2008) Faraday Discuss., 140, pp. 319-335
  • Blanford, C.F., Heath, R.S., Armstrong, F.A., (2007) Chem. Commun., pp. 1710-1712
  • Sosna, M., Chretien, J.-M., Kilburn, J.D., Bartlett, P.N., (2010) Phys. Chem. Chem. Phys., 12, pp. 10018-10026
  • Thorum, M.S., Anderson, C.A., Hatch, J.J., Campbell, A.S., Marshall, N.M., Zimmerman, S.C., Lu, Y., Gewirth, A.A., (2010) J. Phys. Chem. Lett., 1, pp. 2251-2254
  • Szamocki, R., Flexer, V., Levin, L., Forchiasin, F., Calvo, E.J., (2009) Electrochim. Acta, 54, pp. 1970-1977
  • Scodeller, P., Carballo, R., Szamocki, R., Levin, L., Forchiassin, F., Calvo, E.J., (2010) J. Am. Chem. Soc., 132, pp. 11132-11140
  • Decher, G., (1997) Science, 277, pp. 1232-1237
  • Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N., (1997) Langmuir, 13, pp. 2708-2716
  • Calvo, E.J., Etchenique, R., Pietrasanta, L., Wolosiuk, A., Danilowicz, C., (2001) Anal. Chem., 73, pp. 1161-1168
  • Calvo, E.J., Battaglini, F., Danilowicz, C., Wolosiuk, A., Otero, M., (2000) Faraday Discuss., 116, pp. 47-65
  • Calvo, E.J., Flexer, V., Tagliazucchi, M., Scodeller, P., (2010) Phys. Chem. Chem. Phys., 12, pp. 10033-10039
  • Laurent, D., Schlenoff, J.B., (1997) Langmuir, 13, pp. 1552-1557
  • He, P.L., Hu, N.F., Rusling, J.F., (2004) Langmuir, 20, pp. 722-729
  • Caruso, F., Niikura, K., Furlong, D.N., Okahata, Y., (1997) Langmuir, 13, pp. 3422-3426
  • Danilowicz, C., Corton, E., Battaglini, F., (1998) J. Electroanal. Chem., 445, pp. 89-94
  • Shiratori, S.S., Rubner, M.F., (2000) Macromolecules, 33, pp. 4213-4219
  • Folkesso, B., (1973) Acta Chem. Scand., 27, pp. 287-302
  • Klein, J.C., Proctor, A., Hercules, D.M., Black, J.F., (1983) Anal. Chem., 55, pp. 2055-2059

Citas:

---------- APA ----------
Scodeller, P., Williams, F.J. & Calvo, E.J. (2014) . XPS analysis of enzyme and mediator at the surface of a layer-by-layer self-assembled wired enzyme electrode. Analytical Chemistry, 86(24), 12180-12184.
http://dx.doi.org/10.1021/ac503147c
---------- CHICAGO ----------
Scodeller, P., Williams, F.J., Calvo, E.J. "XPS analysis of enzyme and mediator at the surface of a layer-by-layer self-assembled wired enzyme electrode" . Analytical Chemistry 86, no. 24 (2014) : 12180-12184.
http://dx.doi.org/10.1021/ac503147c
---------- MLA ----------
Scodeller, P., Williams, F.J., Calvo, E.J. "XPS analysis of enzyme and mediator at the surface of a layer-by-layer self-assembled wired enzyme electrode" . Analytical Chemistry, vol. 86, no. 24, 2014, pp. 12180-12184.
http://dx.doi.org/10.1021/ac503147c
---------- VANCOUVER ----------
Scodeller, P., Williams, F.J., Calvo, E.J. XPS analysis of enzyme and mediator at the surface of a layer-by-layer self-assembled wired enzyme electrode. Anal. Chem. 2014;86(24):12180-12184.
http://dx.doi.org/10.1021/ac503147c