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Abstract:

Azanone (HNO, nitroxyl) is a highly reactive and short-lived compound with intriguing and highly relevant properties. It has been proposed to be a reaction intermediate in several chemical reactions and an in vivo, endogenously produced key metabolite and/or signaling molecule. In addition, its donors have important pharmacological properties. Therefore, given its relevance and elusive nature (it reacts with itself very quickly), the development of reliable analytical methods for quantitative HNO detection is in high demand for the advancement of future research in this area. During the past few years, several methods were developed that rely on chemical reactions followed by mass spectrometry, high-performance liquid chromatography, UV-vis, or fluorescence-trapping-based methodologies. In this work, our recently developed HNO-sensing electrode, based on the covalent attachment of cobalt(II) 5,10,15,20-tetrakis[3-(p-acetylthiopropoxy)phenyl] porphyrin [Co(P)] to a gold electrode, has been thoroughly characterized in terms of sensibility, accuracy, time-resolved detection, and compatibility with complex biologically compatible media. Our results show that the Co(P) electrode: (i) allows time-resolved detection and kinetic analysis of the electrode response (the underlying HNO-producing reactions can be characterized) (ii) is able to selectively detect and reliably quantify HNO in the 1-1000 nM range, and (iii) has good biological media compatibility (including cell culture), displaying a lack of spurious signals due to the presence of O2, NO, and other reactive nitrogen and oxygen species. In summary, the Co(P) electrode is to our knowledge the best prospect for use in studies investigating HNO-related chemical and biological reactions. © 2013 American Chemical Society.

Registro:

Documento: Artículo
Título:Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
Autor:Suárez, S.A.; Bikiel, D.E.; Wetzler, D.E.; Martí, M.A.; Doctorovich, F.
Filiación:Departamento de Química Inorgánica, Analítica y Química, Física/INQUIMAE-CONICET, Universidad de Buenos Aires, C1428EHA, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA, Buenos Aires, Argentina
Palabras clave:Analytical method; Chemical and biologicals; Covalent attachment; Electrode response; Pharmacological properties; Reactive nitrogen and oxygen species; Signaling molecules; Time-resolved detection; Cell culture; Chemical reactions; Gold compounds; High performance liquid chromatography; Mass spectrometry; Cobalt compounds; nitrogen oxide; nitroxyl; article; electrochemical analysis; fluorescence; high performance liquid chromatography; kinetics; limit of detection; mass spectrometry; methodology; ultraviolet spectrophotometry; Chromatography, High Pressure Liquid; Electrochemical Techniques; Fluorescence; Kinetics; Limit of Detection; Mass Spectrometry; Nitrogen Oxides; Spectrophotometry, Ultraviolet
Año:2013
Volumen:85
Número:21
Página de inicio:10262
Página de fin:10269
DOI: http://dx.doi.org/10.1021/ac402134b
Título revista:Analytical Chemistry
Título revista abreviado:Anal. Chem.
ISSN:00032700
CODEN:ANCHA
CAS:nitrogen oxide, 11104-93-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v85_n21_p10262_Suarez

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Citas:

---------- APA ----------
Suárez, S.A., Bikiel, D.E., Wetzler, D.E., Martí, M.A. & Doctorovich, F. (2013) . Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level. Analytical Chemistry, 85(21), 10262-10269.
http://dx.doi.org/10.1021/ac402134b
---------- CHICAGO ----------
Suárez, S.A., Bikiel, D.E., Wetzler, D.E., Martí, M.A., Doctorovich, F. "Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level" . Analytical Chemistry 85, no. 21 (2013) : 10262-10269.
http://dx.doi.org/10.1021/ac402134b
---------- MLA ----------
Suárez, S.A., Bikiel, D.E., Wetzler, D.E., Martí, M.A., Doctorovich, F. "Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level" . Analytical Chemistry, vol. 85, no. 21, 2013, pp. 10262-10269.
http://dx.doi.org/10.1021/ac402134b
---------- VANCOUVER ----------
Suárez, S.A., Bikiel, D.E., Wetzler, D.E., Martí, M.A., Doctorovich, F. Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level. Anal. Chem. 2013;85(21):10262-10269.
http://dx.doi.org/10.1021/ac402134b