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Abstract:

The activation of a lipopolysaccharide (LPS) with 1-cyano-4- dimethylaminopyridinium tetrafluoroborate (CDAP) in the presence of a surfactant allows an efficient conjugation with dansyl hydrazine or horseradish peroxidase (HRP) in an aqueous medium maintaining its biological activity. In order to promote the reaction a series of amphiphilic compounds were tested, sodium deoxycholate being the most suitable. The method presents several advantages: it is carried out in a mild environment, good conjugation ratios are obtained, it is suitable for any label bearing amino, hydrazine, or hydrazide groups, and the LPS endotoxic and HRP enzymatic activities are preserved. The HRP conjugate is applied in an amperometric competitive assay for the detection of lipopolysaccharides in an electrode array combined with a multipotentiostat able to carry out simultaneous determinations. The system is able to detect samples in concentrations as low as 100 pg mL-1 of LPS. © 2009 American Chemical Society.

Registro:

Documento: Artículo
Título:Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay
Autor:Pallarola, D.; Battaglini, F.
Filiación:INQUIMAE, Departamento de Química Inorgá Nica, Analítica Y Química Física, Pabellón 2, C1428EHA Buenos Aires, Argentina
Palabras clave:Amperometric; Amphiphilic compound; Aqueous medium; Biological activities; Electrode arrays; Enzymatic activities; Horseradish peroxidase; HRP conjugate; Hydrazide groups; Lipopolysaccharide; Lipopolysaccharides; Simultaneous determinations; Sodium deoxycholate; Tetrafluoroborate; Amines; Hydrazine; Sodium; Surface active agents; Polysaccharides; 1 cyano 4 dimethylaminopyridinium tetrafluoroborate; deoxycholate sodium; lipopolysaccharide; pyridinium derivative; unclassified drug; article; bacterial strain; chemical reaction; chemical structure; concentration (parameters); controlled study; enzyme activity; nonhuman; pH measurement; Binding, Competitive; Dansyl Compounds; Electrochemical Techniques; Electrodes; Horseradish Peroxidase; Hydrazines; Lipopolysaccharides; Nitriles; Pyridinium Compounds; Surface-Active Agents
Año:2009
Volumen:81
Número:10
Página de inicio:3824
Página de fin:3829
DOI: http://dx.doi.org/10.1021/ac9001639
Título revista:Analytical Chemistry
Título revista abreviado:Anal. Chem.
ISSN:00032700
CODEN:ANCHA
CAS:deoxycholate sodium, 302-95-4; 1-cyano-4-dimethylaminopyridinium, 59016-55-6; Dansyl Compounds; Horseradish Peroxidase, 1.11.1.-; Hydrazines; Lipopolysaccharides; Nitriles; Pyridinium Compounds; Surface-Active Agents; dansyl hydrazine, 33008-06-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v81_n10_p3824_Pallarola

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Citas:

---------- APA ----------
Pallarola, D. & Battaglini, F. (2009) . Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay. Analytical Chemistry, 81(10), 3824-3829.
http://dx.doi.org/10.1021/ac9001639
---------- CHICAGO ----------
Pallarola, D., Battaglini, F. "Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay" . Analytical Chemistry 81, no. 10 (2009) : 3824-3829.
http://dx.doi.org/10.1021/ac9001639
---------- MLA ----------
Pallarola, D., Battaglini, F. "Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay" . Analytical Chemistry, vol. 81, no. 10, 2009, pp. 3824-3829.
http://dx.doi.org/10.1021/ac9001639
---------- VANCOUVER ----------
Pallarola, D., Battaglini, F. Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay. Anal. Chem. 2009;81(10):3824-3829.
http://dx.doi.org/10.1021/ac9001639