Artículo
Calvo, E.J.; Etchenique, R.; Pietrasanta, L.; Wolosiuk, A.; Danilowicz, C. "Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode" (2001) Analytical Chemistry. 73(6):1161-1168
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Abstract:
The uptake of glucose oxidase (GOx) onto a polycationic redox polymer (PAA-Os)-modified surface, by adsorption from dilute aqueous GOx solutions, was followed by the quartz crystal microbalance (QCM) and shows double exponential kinetics. The electrochemistry of the layer-by-layer-deposited redox-active polymer was followed by cyclic voltammetry in glucose-free solutions, and the enzyme catalysis mediated by the redox polymer was studied in β-D-glucose-containing solutions. AFM studies of the different layers showed the existence of large two dimension enzyme aggregates on the osmium polymer for 1 μM GOx and less aggregation for 50 nM GOx solutions. When the short alkanethiol, 2,2′-diaminoethyldisulfide was preadsorbed onto gold, a monoexponential adsorption law was observed, and single GOx enzyme molecules could be seen on the surface where the enzyme was adsorbed from 50 nM GOx in water.
Registro:
| Documento: | Artículo |
| Título: | Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode |
| Autor: | Calvo, E.J.; Etchenique, R.; Pietrasanta, L.; Wolosiuk, A.; Danilowicz, C. |
| Filiación: | INQUIMAE, Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, Pabellon 2 Ciudad Universitaria, AR-1428 Buenos Aires, Argentina Permanent research staff CONICET, Argentina Physics Department, University of California, Santa Barbara, CA 93106, United States Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires-Junin, 956 AR-1113 Buenos Aires, Argentina |
| Palabras clave: | Redox polymers; Adsorption; Agglomeration; Cyclic voltammetry; Electrochemistry; Electrodes; Glucose; Gold; Polymers; Quartz; Self assembly; Enzymes; 2,2' diaminoethyldisulfide; alkane derivative; beta glucose; enzyme; glucose; glucose derivative; glucose oxidase; gold; osmium derivative; polyamine derivative; polycation; polymer; silicon dioxide; unclassified drug; water; adsorption; aqueous solution; article; atomic force microscopy; cyclic potentiometry; electrochemistry; electrode; kinetics; oxidation reduction reaction; protein assembly; Biosensing Techniques; Electrochemistry; Glucose Oxidase; Organometallic Compounds; Oxidation-Reduction |
| Año: | 2001 |
| Volumen: | 73 |
| Número: | 6 |
| Página de inicio: | 1161 |
| Página de fin: | 1168 |
| DOI: | http://dx.doi.org/10.1021/ac0011686 |
| Título revista: | Analytical Chemistry |
| Título revista abreviado: | Anal. Chem. |
| ISSN: | 00032700 |
| CODEN: | ANCHA |
| CAS: | glucose, 50-99-7, 84778-64-3; glucose oxidase, 9001-37-0; gold, 7440-57-5; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; water, 7732-18-5; Glucose Oxidase, 1.1.3.4; Organometallic Compounds; Os(Bpy)2CIPyCH2NH-poly(allylamine) |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v73_n6_p1161_Calvo |
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Citas:
---------- APA ----------
Calvo, E.J., Etchenique, R., Pietrasanta, L., Wolosiuk, A. & Danilowicz, C. (2001) . Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode. Analytical Chemistry, 73(6), 1161-1168.http://dx.doi.org/10.1021/ac0011686
---------- CHICAGO ----------
Calvo, E.J., Etchenique, R., Pietrasanta, L., Wolosiuk, A., Danilowicz, C. "Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode" . Analytical Chemistry 73, no. 6 (2001) : 1161-1168.http://dx.doi.org/10.1021/ac0011686
---------- MLA ----------
Calvo, E.J., Etchenique, R., Pietrasanta, L., Wolosiuk, A., Danilowicz, C. "Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode" . Analytical Chemistry, vol. 73, no. 6, 2001, pp. 1161-1168.http://dx.doi.org/10.1021/ac0011686
---------- VANCOUVER ----------
Calvo, E.J., Etchenique, R., Pietrasanta, L., Wolosiuk, A., Danilowicz, C. Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode. Anal. Chem. 2001;73(6):1161-1168.http://dx.doi.org/10.1021/ac0011686
