Navegar

Colección

Datos Estadísticas

Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

We report here a novel bioelectrode based on self-assembled multilayers of polyphenol oxidase intercalated with cationic polyallylamine built up on a thiol-modified gold surface. We use an immobilization strategy previously described by Hodak J. et al. (Langmuir 1997, 13, 2708-2716) Quartz crystal microbalance with electroacustic impedance experiments were carried oat to follow quantitatively the multilayer film formation. The response of the self-assembly polyphenol oxidase-polyallylamine electrodes toward different metabolically related catecholamines was studied, to evaluate enzyme kinetics. For the analyzed compounds, only dopamine and its metabolite Dopac gave catalytic currents at applied potential close to 0 V. These responses were proportional to the number of polyphenol oxidase-immobilized layers and were also controlled by the enzymatic reaction. The combination of microgravimetric and electrochemical techniques allowed us to determine the kinetic enzymatic constants, showing that the decomposition rate for the enzyme-substrate complex is slower than the enzymatic reoxidation step.

Registro:

Documento: Artículo
Título:Electrochemical behavior of polyphenol oxidase immobilized in self-assembled structures layer by layer with cationic polyallylamine
Autor:Forzani, E.S.; Solís, V.M.; Calvo, E.J.
Filiación:INFIQC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Pabellón, Argentina
INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Argentina
AR-1428, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:allylamine derivative; catechol oxidase; cation; Argentina; article; electrochemical analysis; electrochemistry; electrode; immobilization; surface property; Catechol Oxidase; Electrochemistry; Enzymes, Immobilized; Indicators and Reagents; Polyamines
Año:2000
Volumen:72
Número:21
Página de inicio:5300
Página de fin:5307
DOI: http://dx.doi.org/10.1021/ac0003798
Título revista:Analytical Chemistry
Título revista abreviado:Anal. Chem.
ISSN:00032700
CODEN:ANCHA
CAS:Catechol Oxidase, 1.10.3.1; Enzymes, Immobilized; Indicators and Reagents; Polyamines; polyallylamine, 30551-89-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v72_n21_p5300_Forzani

Referencias:

  • Scheller, F., Schubert, F., (1992) Biosensors, Techniques and Instrumentation in Analytical Chemistry, 11. , Elsevier Science Publishers B. V.: Amsterdam, London, New York, Tokyo
  • Ghindilis, A.L., Atanasov, P., Wilkins, E., (1997) Electroanalysis, 9, pp. 661-673
  • Bartlett, P.N., Cooper, J.M., (1993) J. Electroanal. Chem., 362, pp. 1-12
  • Bartlett, P.N., Cooper, J.M., Applications of electroative polymers in bioelectrochemistry and bioelectronics (1996), Electroactive Polymers Electrochemistry; Lyons, M. E. G., Ed.; Plenum Press: New York, London; Part 2, Chapter 9; Decher, G., (1997) Science, 277, pp. 1232-1237
  • Lvov, Y., Essler, F., Decher, G., (1993) J. Phys. Chem., 97, pp. 13773-13777
  • Lvov, Y., Decher, G., Mohwald, H., (1993) Langmuir, 9, pp. 481-486
  • Lvov, Y., Decher, G., Sukhorukov, G., (1993) Macromolecules, 26, pp. 5396-5399
  • Decher, G., Lehr, B., Lowack, K., Lvov, Y., Schmitt, J., (1994) Biosens. Bioelectron., 9, pp. 677-684
  • Lvov, Y., Haas, H., Decher, G., Mohwald, H., Mikhailov, A., Mtchedlishvily, B., Morgunona, E., Vainshtein, B., (1994) Langmuir, 10, pp. 4232-4236
  • Lvov, Y., Ariga, K., Ichinose, I., Kunitake, T., (1995) J. Am. Chem. Soc., 117, pp. 6117-6123
  • Onda, M., Lvov, Y., Ariga, K., Kunitake, T., (1996) Biotechnol. Bioeng., 51, pp. 163-167
  • Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N., (1997) Langmuir, 13, pp. 2708-2716
  • Laurent, D., Schelenoff, J.B., (1997) Langmuir, 13, pp. 1552-1557
  • Lvov, Y., Ariga, K., Ichinose, I., Kunitake, T., (1996) Langmuir, 12, pp. 3038-3044
  • Lvov, Y., (2000), p. 125. , Protein Architecture; Lvov Y., Mohwald H., Eds.; Marcel Dekker: New York; Chapter 6; Calvo, E.J., Battaglini, F., Danilowicz, C., Wolosiuk, A., Otero, M., Faraday Discuss., , in press
  • Dalmia, A., Liu, C.C., Savinell, R.F., (1997) J. Electroanal. Chem., 430, pp. 205-214
  • Anzai, J.-I., Takeshita, H., Kobayashi, Y., Osa, T., Hoshi, T., (1998) Anal. Chem., 70, pp. 811-817
  • Forzani, E.S., Rivas, G.A., Solis, V.M., (1995) J. Electroanal. Chem., 382, pp. 33-40
  • Forzani, E.S., Rivas, G.A., Solis, V.M., (1997) J. Electroanal. Chem., 435, pp. 77-84
  • Forzani, E.S., Rivas, G.A., Solis, V.M., (1999) J. Electroanal. Chem., 461, pp. 174-183
  • Technical information of Sigma Chemical Co.: Tyrosinase from mushroom, Prod. No. T7755; Calvo, E.J., Etchenique, R., Barttlet, P.N., Singhal, K., Santamaria, C., (1997) Faraday Discuss., 107, pp. 141-157
  • Calve, E.J., Etchenique, R., (1999) J. Phys. Chem. B, 103, pp. 8944-8950
  • Martin, S.J., Granstaff, V.E., Frye, G.C., (1991) Anal. Chem., 63, pp. 2272-2281
  • Gillespie, J.P., (1991) Comp. Biochem. Physiol., 98 C, pp. 351-358
  • Rickrert, J., Andreas, B., Wolfgang, G., (1997) Anal. Chem., 69, pp. 1441-1448
  • Takehara, K., Takemura, H., Aihara, M., Yoshimura, M., (1996) J. Electroanal. Chem., 404, pp. 179-182
  • Forzani, E.S., (1999), Doctoral Thesis, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba; Desprez, V., Labbe, P., (1996) J. Electroanal. Chem., 415, pp. 191-195

Citas:

---------- APA ----------
Forzani, E.S., Solís, V.M. & Calvo, E.J. (2000) . Electrochemical behavior of polyphenol oxidase immobilized in self-assembled structures layer by layer with cationic polyallylamine. Analytical Chemistry, 72(21), 5300-5307.
http://dx.doi.org/10.1021/ac0003798
---------- CHICAGO ----------
Forzani, E.S., Solís, V.M., Calvo, E.J. "Electrochemical behavior of polyphenol oxidase immobilized in self-assembled structures layer by layer with cationic polyallylamine" . Analytical Chemistry 72, no. 21 (2000) : 5300-5307.
http://dx.doi.org/10.1021/ac0003798
---------- MLA ----------
Forzani, E.S., Solís, V.M., Calvo, E.J. "Electrochemical behavior of polyphenol oxidase immobilized in self-assembled structures layer by layer with cationic polyallylamine" . Analytical Chemistry, vol. 72, no. 21, 2000, pp. 5300-5307.
http://dx.doi.org/10.1021/ac0003798
---------- VANCOUVER ----------
Forzani, E.S., Solís, V.M., Calvo, E.J. Electrochemical behavior of polyphenol oxidase immobilized in self-assembled structures layer by layer with cationic polyallylamine. Anal. Chem. 2000;72(21):5300-5307.
http://dx.doi.org/10.1021/ac0003798