Abstract:
A rapid and simplified method for the isolation of porphyrins from either body fluids or porphyrin solutions is presented. It is based on the use of the anion-exchange resin De Acidite FFiP. Adaptation of this technique for analysis of urinary porphyrin mixtures is described, and the results obtained in working with urine corresponding to human porphyria cutanea tarda, rats with experimental porphyria induced by hexachlorobenzene, and rabbits with lead poisoning are given. © 1977.
Registro:
Documento: |
Artículo
|
Título: | Simplified method for the determination of porphyrins in body fluids |
Autor: | Grinstein, M. |
Filiación: | Laboratorio de Porfirinas Departamento, Quimica Biologica Facultad de Ciencias Exactas, Naturales Universidad de Buenos Aires, Argentina
|
Palabras clave: | porphyrin; methodology; normal human; porphyria cutanea tarda; rat; urine; Animal; Hexachlorobenzene; Human; Lead Poisoning; Methods; Porphyria; Porphyrins; Rabbits; Rats |
Año: | 1977
|
Volumen: | 77
|
Número: | 2
|
Página de inicio: | 577
|
Página de fin: | 580
|
DOI: |
http://dx.doi.org/10.1016/0003-2697(77)90278-0 |
Título revista: | Analytical Biochemistry
|
Título revista abreviado: | Anal. Biochem.
|
ISSN: | 00032697
|
CODEN: | ANBCA
|
CAS: | porphyrin, 24869-67-8; Hexachlorobenzene, 118-74-1; Porphyrins
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v77_n2_p577_Grinstein |
Referencias:
- Grinstein, Schwarz, Watson, (1945) J. Biol. Chem, 157, pp. 323-343
- Sancovich, Lambias, Ferramola, del C. Batlle, Grinstein, (1972) Biochim. Biophys. Acta, 261, pp. 328-331
- Garcia, San Martin de Viale, Tomio, Grinstein, (1973) Biochim. Biophys. Acta, 309, pp. 203-210
- Falk, Benson, (1953) Biochem. J, 55, pp. 101-104
- Salum, del C. Batlle, Grinstein, (1961) An. Asoc. Quim. Argent, 49, pp. 3-12
- Nacht, San Martin de Viale, Grinstein, (1970) Clin. Chim. Acta, 27, pp. 445-452
- Doss, Meinhof, Look, Henning, Nawrocki, Dolle, Strohmeyer, Filippini, (1971) S. Afr. J. Lab. Clin. Med, p. 5025. , special issue
- San Martin de Viale, Viale, Nacht, Grinstein, (1970) Clin. Chim. Acta, 28, pp. 13-23
- San Martin de Viale, del C. Rios de Molina, Wainstok, de Calmanovici, Tomio, (1976) International Porphyrin Meeting, Porphyrins in Human Diseases, pp. 445-452. , Freiburg, W. Germany, Karger, Basel, Part 1
- Schmid, Hanson, Schwarz, Experimental Porphyria. I. Isolation of Uroporphyrin I from Bone Marrow of Lead Poisoned Rabbits. (1952) Experimental Biology and Medicine, 79, pp. 459-462
- Bashour, (1954) J. Lab Clin. Med, 44, p. 764
Citas:
---------- APA ----------
(1977)
. Simplified method for the determination of porphyrins in body fluids. Analytical Biochemistry, 77(2), 577-580.
http://dx.doi.org/10.1016/0003-2697(77)90278-0---------- CHICAGO ----------
Grinstein, M.
"Simplified method for the determination of porphyrins in body fluids"
. Analytical Biochemistry 77, no. 2
(1977) : 577-580.
http://dx.doi.org/10.1016/0003-2697(77)90278-0---------- MLA ----------
Grinstein, M.
"Simplified method for the determination of porphyrins in body fluids"
. Analytical Biochemistry, vol. 77, no. 2, 1977, pp. 577-580.
http://dx.doi.org/10.1016/0003-2697(77)90278-0---------- VANCOUVER ----------
Grinstein, M. Simplified method for the determination of porphyrins in body fluids. Anal. Biochem. 1977;77(2):577-580.
http://dx.doi.org/10.1016/0003-2697(77)90278-0