A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic acid-treated filter paper are introduced into a test tube containing the reaction mixture. The papers are submitted to a batch washing-procedure that ensures the removal of the compounds that are not trichloroacetic acid-precipitable. After scintillation counting, the paper can be reused to study the acid lability of the glucoprotein. © 1975.
Documento: | Artículo |
Título: | Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
Autor: | Krisman, C.R.; Barengo, R. |
Filiación: | Instituto de Investigaciones Bioquimicas Fundacion Campomar, Obligado, 2490 Buenos Aires (28), Argentina Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina |
Palabras clave: | carbon; glycoprotein; uridine diphosphate glucose; uridine diphosphate sugar; animal; article; biosynthesis; kinetics; liver; metabolism; paper; rat; technique; time; Animal; Carbon Radioisotopes; Evaluation Studies; Glycoproteins; Kinetics; Liver; Methods; Paper; Rats; Time Factors; Uridine Diphosphate Glucose; Uridine Diphosphate Sugars |
Año: | 1975 |
Volumen: | 66 |
Número: | 2 |
Página de inicio: | 584 |
Página de fin: | 589 |
DOI: | http://dx.doi.org/10.1016/0003-2697(75)90625-9 |
Título revista: | Analytical Biochemistry |
Título revista abreviado: | Anal. Biochem. |
ISSN: | 00032697 |
CODEN: | ANBCA |
CAS: | carbon, 7440-44-0; uridine diphosphate glucose, 133-89-1; Carbon Radioisotopes; Glycoproteins; Uridine Diphosphate Glucose, 133-89-1; Uridine Diphosphate Sugars |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v66_n2_p584_Krisman |