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Abstract:

A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic acid-treated filter paper are introduced into a test tube containing the reaction mixture. The papers are submitted to a batch washing-procedure that ensures the removal of the compounds that are not trichloroacetic acid-precipitable. After scintillation counting, the paper can be reused to study the acid lability of the glucoprotein. © 1975.

Registro:

Documento: Artículo
Título:Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique
Autor:Krisman, C.R.; Barengo, R.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Campomar, Obligado, 2490 Buenos Aires (28), Argentina
Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina
Palabras clave:carbon; glycoprotein; uridine diphosphate glucose; uridine diphosphate sugar; animal; article; biosynthesis; kinetics; liver; metabolism; paper; rat; technique; time; Animal; Carbon Radioisotopes; Evaluation Studies; Glycoproteins; Kinetics; Liver; Methods; Paper; Rats; Time Factors; Uridine Diphosphate Glucose; Uridine Diphosphate Sugars
Año:1975
Volumen:66
Número:2
Página de inicio:584
Página de fin:589
DOI: http://dx.doi.org/10.1016/0003-2697(75)90625-9
Título revista:Analytical Biochemistry
Título revista abreviado:Anal. Biochem.
ISSN:00032697
CODEN:ANBCA
CAS:carbon, 7440-44-0; uridine diphosphate glucose, 133-89-1; Carbon Radioisotopes; Glycoproteins; Uridine Diphosphate Glucose, 133-89-1; Uridine Diphosphate Sugars
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v66_n2_p584_Krisman

Referencias:

  • Bollum, (1959) J. Biol. Chem, 234, p. 2733
  • Bollum, (1968) Methods in Enzymology, 12, p. 169. , S.P. Colowick, N.O. Kaplan, Academic Press, New York
  • Mans, Novelli, (1961) Arch. Biochem. Biophys, 94, p. 48
  • Thomas, Schlender, Larner, (1968) Anal. Biochem, 25, p. 486
  • Byfield, Scherbaum, (1966) Anal. Biochem, 17, p. 434
  • Krisman, Barengo, (1975) Eur. J. Biochem, 52, p. 117
  • Krisman, (1973) Ann. N.Y. Acad. Sci, 210, p. 81
  • Rothman-Denes, Muthukrishnan, Haselkorn, Studier, (1973) Virus Research, p. 227. , C.F. Fox, W.S. Robinson, Academic Press, New York
  • Wright, Robbins, (1965) Biochim. Biophys. Acta, 104, p. 594
  • Krisman, (1972) Biochem. Biophys. Res. Commun, 46, p. 1206

Citas:

---------- APA ----------
Krisman, C.R. & Barengo, R. (1975) . Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique. Analytical Biochemistry, 66(2), 584-589.
http://dx.doi.org/10.1016/0003-2697(75)90625-9
---------- CHICAGO ----------
Krisman, C.R., Barengo, R. "Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique" . Analytical Biochemistry 66, no. 2 (1975) : 584-589.
http://dx.doi.org/10.1016/0003-2697(75)90625-9
---------- MLA ----------
Krisman, C.R., Barengo, R. "Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique" . Analytical Biochemistry, vol. 66, no. 2, 1975, pp. 584-589.
http://dx.doi.org/10.1016/0003-2697(75)90625-9
---------- VANCOUVER ----------
Krisman, C.R., Barengo, R. Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique. Anal. Biochem. 1975;66(2):584-589.
http://dx.doi.org/10.1016/0003-2697(75)90625-9