A specific spectrophotometric determination of β-glucose-1-phosphate has been devised. It allows β-glucose-1-phosphate to be measured in the presence of α-glucose-1-phosphate and of a one hundred-fold excess of glucose-6-phosphate. Phosphoglucomutase for β-glucose-1-phosphate obtained from cells of Euglena gracilis var. bacillaris must be prepared for the assay. © 1973.
| Documento: | Artículo |
| Título: | A specific method for the quantitative determination of β-glucose-1-phosphate |
| Autor: | Belocopitow, E.; Maréchal, L.R. |
| Filiación: | Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado, 2490 Buenos Aires 28, Argentina |
| Palabras clave: | glucose 1 phosphate; phosphoglucomutase; methodology; Animal; Arsenic; Borohydrides; Enzyme Activation; Euglena gracilis; Glucosephosphates; Hydrogen-Ion Concentration; Kinetics; Methods; Phosphoglucomutase; Precipitation; Protamines; Stereoisomerism; Sulfuric Acids |
| Año: | 1973 |
| Volumen: | 53 |
| Número: | 1 |
| Página de inicio: | 108 |
| Página de fin: | 114 |
| DOI: | http://dx.doi.org/10.1016/0003-2697(73)90411-9 |
| Título revista: | Analytical Biochemistry |
| Título revista abreviado: | Anal. Biochem. |
| ISSN: | 00032697 |
| CODEN: | ANBCA |
| CAS: | glucose 1 phosphate, 59-56-3; phosphoglucomutase, 9001-81-4; Arsenic, 7440-38-2; Borohydrides; Glucosephosphates; Phosphoglucomutase, EC 5.4.2.2; Protamines; Sulfuric Acids |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v53_n1_p108_Belocopitow |
