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Abstract:

Optimization of bead analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) after the screening of one-bead-one-peptide combinatorial libraries was achieved, involving the fine-tuning of the whole process. Guanidine was replaced by acetonitrile (MeCN)/acetic acid (AcOH)/water (H2O), improving matrix crystallization. Peptide-bead cleavage with NH4OH was cheaper and safer than, yet as efficient as, NH3/tetrahydrofuran (THF). Peptide elution in microtubes instead of placing the beads in the sample plate yielded more sample aliquots. Successive dry layers deposit sample preparation was better than the dried droplet method. Among the matrices analyzed, α-cyano-4-hydroxycinnamic acid resulted in the best peptide ion yield. Cluster formation was minimized by the addition of additives to the matrix. © 2010 Elsevier Inc.

Registro:

Documento: Artículo
Título:Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Autor:Martínez-Ceron, M.C.; Giudicessi, S.L.; Marani, M.M.; Albericio, F.; Cascone, O.; Erra-Balsells, R.; Camperi, S.A.
Filiación:Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, 1113 Buenos Aires, Argentina
CIHDECAR-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
IRB Barcelona, CIBER-BBN, 08028 Barcelona, Spain
Department of Organic Chemistry, University of Barcelona, 08028 Barcelona, Spain
Palabras clave:acetic acid; guanidine; tetrahydrofuran; trifluoroacetic acid; urea; ambient air; article; crystallization; elution; ionization; matrix assisted laser desorption ionization time of flight mass spectrometry; peptide analysis; priority journal; process optimization; sensitivity analysis; tandem mass spectrometry; Acetic Acid; Acetonitriles; Hydroxides; Peptide Library; Peptides; Sequence Analysis, Protein; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Water
Año:2010
Volumen:400
Número:2
Página de inicio:295
Página de fin:297
DOI: http://dx.doi.org/10.1016/j.ab.2010.01.029
Título revista:Analytical Biochemistry
Título revista abreviado:Anal. Biochem.
ISSN:00032697
CODEN:ANBCA
CAS:acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; guanidine, 113-00-8, 25215-10-5, 50-01-1; tetrahydrofuran, 109-99-9; trifluoroacetic acid, 14477-72-6, 2923-18-4, 76-05-1; urea, 57-13-6; Acetic Acid, 64-19-7; Acetonitriles; Hydroxides; Peptide Library; Peptides; Water, 7732-18-5; acetonitrile, 75-05-8; ammonium hydroxide, 1336-21-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v400_n2_p295_MartinezCeron

Referencias:

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Citas:

---------- APA ----------
Martínez-Ceron, M.C., Giudicessi, S.L., Marani, M.M., Albericio, F., Cascone, O., Erra-Balsells, R. & Camperi, S.A. (2010) . Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Analytical Biochemistry, 400(2), 295-297.
http://dx.doi.org/10.1016/j.ab.2010.01.029
---------- CHICAGO ----------
Martínez-Ceron, M.C., Giudicessi, S.L., Marani, M.M., Albericio, F., Cascone, O., Erra-Balsells, R., et al. "Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry" . Analytical Biochemistry 400, no. 2 (2010) : 295-297.
http://dx.doi.org/10.1016/j.ab.2010.01.029
---------- MLA ----------
Martínez-Ceron, M.C., Giudicessi, S.L., Marani, M.M., Albericio, F., Cascone, O., Erra-Balsells, R., et al. "Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry" . Analytical Biochemistry, vol. 400, no. 2, 2010, pp. 295-297.
http://dx.doi.org/10.1016/j.ab.2010.01.029
---------- VANCOUVER ----------
Martínez-Ceron, M.C., Giudicessi, S.L., Marani, M.M., Albericio, F., Cascone, O., Erra-Balsells, R., et al. Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal. Biochem. 2010;400(2):295-297.
http://dx.doi.org/10.1016/j.ab.2010.01.029